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This study generated an antisense single-stranded DNA complementary to the PTK6 coding sequence via asymmetric PCR, purified it, and encapsulated it in cationic nanoliposomes. Particle size, morphology, and zeta potential were characterized, and PANC-1 and HPDE-C7 cells were treated for up to 72 h. CNLAPCRP reduced PANC-1 viability, induced apoptosis, decreased PTK6 protein levels, and showed stronger effects in cancer cells than in non-cancerous cells, supporting targeted, sequence-specific gene silencing while further mechanistic validation is required.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/the-cationic-nanoliposomes-loaded-with-asymmetric-pcr-product-targeting-ptk6-mrna-and-induce-apoptosis-in-panc-1-cells/345962/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/the-cationic-nanoliposomes-loaded-with-asymmetric-pcr-product-targeting-ptk6-mrna-and-induce-apoptosis-in-panc-1-cells/345962.png","ImageObject",300,407,{"name":92,"@type":93},"Evangeline","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-23","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":8},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"How was the antisense targeting material for PTK6 prepared?","Question",{"text":112,"@type":113},"An antisense single-stranded DNA complementary to the PTK6 coding sequence was generated using asymmetric PCR, purified, and encapsulated into cationic nanoliposomes (CNLAPCRP).","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What were CNLAPCRP’s key physicochemical characteristics?",{"text":117,"@type":113},"CNLAPCRP formed near-spherical particles with a size range of about 30–120 nm (mean ~60 nm) and a zeta potential around +25 mV.",{"name":119,"@type":110,"acceptedAnswer":120},"How did CNLAPCRP affect pancreatic cancer and non-cancer cells?",{"text":121,"@type":113},"CNLAPCRP significantly reduced PANC-1 cell viability and induced apoptosis while decreasing PTK6 protein levels, and it showed a stronger effect on cancer cells (PANC-1) than on non-cancerous cells (HPDE-C7).","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},345962,1790154892,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":8,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},13056703019662,"https://ap-avatar.wpscdn.com/avatar/be000253a8e92610077?_k=1778726343310543188","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nThe cationic nanoliposomes loaded with asymmetric PCR product targeting PTK6 mRNA and induce apoptosis in PANC-1 cells  \nReyhane FooladiVayqan1, Maliheh Entezari1,2, RaziyeTajali3, Neda Zali3, Ali Jebali1,4􀀍, Amir Sadeghi3,5􀀍 & Mehrdad Hashemi1,2􀀍  \nProtein tyrosine kinase 6 (PTK6/BRK) is implicated in tumor cell survival and has emerged as a potential therapeutic target in pancreatic cancer. Here, the aim of this study was to evaluate the cationic nanoliposomes loaded with asymmetric PCR product (CNLAPCRP) targeting PTK6 in pancreatic cancerous cells (PANC-1) than on pancreatic non-cancerous cells (HPDE-C7) . Here, an antisense single-stranded DNA complementary to the PTK6 coding sequence was generated by asymmetric PCR, purified, and encapsulated into cationic nanoliposomes. Their particle size, morphology, and surface charge were characterized. Both PANC-1 and HPDE-C7 cells were exposed toCNLAPCRP (0, 0.1, 1, and 10 µg/mL), Asymetric PCR product alone (0, 0.1, 1, and 10 µg/mL), nanoliposome alone, and PBS as negative control for 24, 48, and 72 h. Finally, the cell viability (MTT), apoptosis (flow cytometry), and western bloting were assessed done for all study groups. The CNLAPCRPs were near-spherical (30–120 nm; mean ~ 60 nm) with a zeta potential of ~ + 25 mV. Here, we found that CNLAPCRP effectively reduced PANC-1 cell viability, induces apoptosis, and decreased ptk6 protein level. The most important finding was that CNLAPCRP had a much greater effect on cancerous cells (PANC-1) than on non-cancerous cells (HPDE-C7), indicating targeted manner ofCNLAPCRP. While these data support the biological activity of the CNLAPCRP, direct demonstration of PTK6 knockdown and inclusion of sequence-matched and formulation controls are needed to confirm target-specific gene silencing and to delineate the mechanism. Further in-depth characterization and in vivo evaluation are warranted.  \nKeywords PTK6, Asymmetric PCR, Antisense oligonucleotide, Nanoliposome, Pancreatic cancer, PANC‑1, Apoptosis, Gene targeting  \nPancreatic adenocarcinoma (PAAD) is among the most delicate solid malignancies, largely because of its late onset, rapid progression, and lack of effective therapeutic options1,2. Standard treatments only slightly improve survival, and current biomarkers are not sensitive or specific enough for early detection. These facts highlight the need for precision modalities and novel molecular targets that can inhibit PAAD’s carcinogenic signaling pathways3.  \nThe PTK6 gene, also referred to as Breast Tumor Kinase (BRK), encodes a non‑receptor cytoplasmic tyrosine kinase primarily found in differentiated epithelial tissues. This protein features three structural domains—SH3, SH2, and a catalytic kinase domain—that allow it to interact with a diverse array of intracellular signaling molecules4. PTK6 expression is normally restricted to mature and differentiated tissues, where it is essential for processes like cell migration, survival, stress response, differentiation, and growth. However, studies show that PTK6 expression is markedly upregulated in colorectal, breast, prostate, esophageal, and pancreatic cancers, among other cancer types5–9. Its upregulation has been linked to tumor formation, cancer progression, increased cell motility, and metastasis9. Mechanistically, PTK6 advances the development of cancer by activating important  \n1Farhikhtegan Medical Convergence Sciences Research Center, Farhikhtegan Hospital, Faculty of medicine,TeMS. C, Islamic Azad University, Tehran, Iran. 2Department of Genetics, Faculty of medicine,TeMS. C, Islamic Azad University, Tehran, Iran. 3Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 4Department of Nanobiotechnology, TeMS. C, Islamic Azad University, Tehran, Iran. 5Basic and Molecular Epidemiol","cbCaiuHL1QCnoXBn","https://ap.wps.com/l/cbCaiuHL1QCnoXBn","pdf",5916228,13,"English","# Background and Rationale\n## Pancreatic adenocarcinoma and need for new targets\n## PTK6 function and therapeutic relevance\n# Study Design and Methods\n## Antisense generation by asymmetric PCR\n## Nanoliposome formulation and characterization\n## Cell treatment and assay plan\n# Key Results and Interpretation\n## Physicochemical properties of CNLAPCRP\n## Effects on cell viability and apoptosis\n## PTK6 protein reduction and cell selectivity\n# Limitations and Future Work","[{\"question\":\"How was the antisense targeting material for PTK6 prepared?\",\"answer\":\"An antisense single-stranded DNA complementary to the PTK6 coding sequence was generated using asymmetric PCR, purified, and encapsulated into cationic nanoliposomes (CNLAPCRP).\"},{\"question\":\"What were CNLAPCRP’s key physicochemical characteristics?\",\"answer\":\"CNLAPCRP formed near-spherical particles with a size range of about 30–120 nm (mean ~60 nm) and a zeta potential around +25 mV.\"},{\"question\":\"How did CNLAPCRP affect pancreatic cancer and non-cancer cells?\",\"answer\":\"CNLAPCRP significantly reduced PANC-1 cell viability and induced apoptosis while decreasing PTK6 protein levels, and it showed a stronger effect on cancer cells (PANC-1) than on non-cancerous cells (HPDE-C7).\"}]","The cationic nanoliposomes loaded with asymmetric PCR product targeting PTK6 mRNA and induce apoptosis in PANC-1 cells | PDF",1790059540,33]