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Mechanistically, rScyreprocin is membrane-active: it triggers reactive oxygen species generation, induces endoplasmic reticulum stress and Ca2+ release, disrupts mitochondria, activates caspase-3 cascades, and culminates in apoptosis. In vivo, intratumoral rScyreprocin inhibits H460 xenografts with efficacy comparable to cisplatin, supporting lung cancer treatment potential.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/the-anticancer-activity-conferred-by-the-mud-crab-antimicrobial-peptide-scyreprocin-through-apoptosis-and-membrane-disruption/512940/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/the-anticancer-activity-conferred-by-the-mud-crab-antimicrobial-peptide-scyreprocin-through-apoptosis-and-membrane-disruption/512940.png","ImageObject",300,407,{"name":42,"@type":43},"Aran","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-10","2026-10-06",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",11,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What is Scyreprocin and where was it originally identified?","Question",{"text":63,"@type":64},"Scyreprocin is an antimicrobial peptide first identified in the mud crab Scylla paramamosain.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How does rScyreprocin inhibit lung cancer cells?",{"text":68,"@type":64},"rScyreprocin acts as a membrane-active peptide that induces ROS, endoplasmic reticulum stress and Ca2+ release, leading to mitochondrial dysfunction, caspase-3 activation, and apoptosis.",{"name":70,"@type":61,"acceptedAnswer":71},"How effective is rScyreprocin in vivo compared with cisplatin?",{"text":72,"@type":64},"Intratumoral injection of rScyreprocin significantly inhibits H460 xenograft growth, with an inhibition rate of 69.94% versus 80.76% for cisplatin.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},512940,1791404839,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,120,123,127],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},8,"Research & Report",30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":124,"doc_module":4,"doc_module_name":25,"category_name":125,"show_sort_weight":124,"slug":126},10,"Lifestyle","lifestyle",{"id":128,"doc_module":4,"doc_module_name":25,"category_name":129,"show_sort_weight":97,"slug":130},19,"General","general",{"code":4,"msg":82,"data":132},{"doc_id":79,"user_id":133,"nickname":42,"user_avatar":134,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":140,"language":141,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":12,"update_tm":145,"read_time":146,"preview_page_count":4},137455076865,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","Article  \nThe Anticancer Activity Conferred by the Mud Crab Antimicrobial Peptide Scyreprocin through Apoptosis and Membrane Disruption  \nYing Yang 1, Hui-Yun Chen 1,2,3, Hua Hao 1,2,3 and Ke-Jian Wang 1,2,3, *  \nCitation: Yang, Y.; Chen, H.-Y.; Hao, H.; Wang, K.-J. The Anticancer Activity Conferred by the Mud Crab Antimicrobial Peptide Scyreprocin through Apoptosis and Membrane Disruption. Int. J. Mol. Sci. 2022, 23, 5500. [https://doi.org/10.3390/](https://doi.org/10.3390/)[ ](https://doi.org/10.3390/)ijms23105500  \nAcademic Editor: Giovanna Batoni  \nReceived: 17 April 2022  \nAccepted: 11 May 2022  \nPublished: 14 May 2022  \nPublisher's Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.  \nCopyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China; [yangying0803@gmail.com](yangying0803@gmail.com) (Y.Y.); [hychen@xmu.edu.cn](hychen@xmu.edu.cn) (H.-Y.C.); [hhao@xmu.edu.cn](hhao@xmu.edu.cn) (H.H.)  \n2 State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China  \n3 Fujian Innovation Research Institute for Marine Biological Antimicrobial Peptide Industrial Technology, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, China  \n* [Correspondence: wkjian@xmu.edu.cn](Correspondence: wkjian@xmu.edu.cn)  \nAbstract: Scyreprocin is an antimicrobial peptide first identified in the mud crab Scylla paramamosain. Herein, we showed that its recombinant product (rScyreprocin) could significantly inhibit the growth of human lung cancer NCI-H460 cells (H460), but showed no cytotoxicity to human lung fibroblasts (HFL1) . rScyreprocin was a membrane-active peptide that firstly induced the generation of reactive oxygen species (ROS) in H460, and led to endoplasmic reticulum stress and Ca2+ release, which resulted in mitochondrial dysfunction and subsequently activation of caspase-3 cascades, and ultimately led to apoptosis. The comprehensive results indicated that rScyreprocin exerted anticancer activity by disrupting cell membrane and inducing apoptosis. The in vivo efficacy test demonstrated that intratumoral injection of rScyreprocin significantly inhibited the growth of H460 xenografts, which was close to that of the cisplatin (inhibition rate: 69.94% vs. 80.76%) . Therefore, rScyreprocin is expected to become a promising candidate for the treatment of lung cancer.  \nKeywords: scyreprocin; antimicrobial peptide; apoptosis; anti-tumor; non-small-cell lung cancer  \n1. Introduction  \nCancer is a major public health problem and the leading cause of death worldwide. With ~2.1 million cases in 2018, lung cancer is the most commonly diagnosed and fatal cancer for men and women worldwide [1] . Although the death rate of lung cancer has declined in some countries, the deaths caused by lung cancer have surpassed that of other major cancers combined in 2017 [2] . There are two main subtypes of lung cancer: small-cell lung carcinoma and non-small-cell lung carcinoma (NSCLC) . NSCLC accounts for about 85% of total lung cancer cases [3] . For the patients with stage I, II, or IIIA NSCLC, surgery can be performed [4] . About 40% of newly diagnosed NSCLC patients are in stage IV. Due to the metastasis of NSCLC, the surgery is less effective; thus, chemotherapy and radiotherapy are usually recommended as the first-line treatment [5] .  \nAlthough new treatments (e.g., immunotherapy) have been rapidly developed in recent years, platinum-based chemotherapy is still the prefe","cbCaieWpVxqxYpRU","https://ap.wps.com/l/cbCaieWpVxqxYpRU","pdf",5246560,17,"English","# Abstract\n# Keywords\n# 1. Introduction","[{\"question\":\"What is Scyreprocin and where was it originally identified?\",\"answer\":\"Scyreprocin is an antimicrobial peptide first identified in the mud crab Scylla paramamosain.\"},{\"question\":\"How does rScyreprocin inhibit lung cancer cells?\",\"answer\":\"rScyreprocin acts as a membrane-active peptide that induces ROS, endoplasmic reticulum stress and Ca2+ release, leading to mitochondrial dysfunction, caspase-3 activation, and apoptosis.\"},{\"question\":\"How effective is rScyreprocin in vivo compared with cisplatin?\",\"answer\":\"Intratumoral injection of rScyreprocin significantly inhibits H460 xenograft growth, with an inhibition rate of 69.94% versus 80.76% for cisplatin.\"}]","The Anticancer Activity Conferred by the Mud Crab Antimicrobial Peptide Scyreprocin through Apoptosis and Membrane Disruption | PDF",1791277908,43]