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In MDA-MB-231 cells, CDs/ZnO reduces viability, proliferation, migration, invasion, adhesion, and clonogenicity, triggers cell-cycle arrest and apoptosis, disrupts redox homeostasis, alters metabolome and transcriptome via MAPK signaling. In TNBC xenograft mice, tumor growth is markedly suppressed with supported 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models.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},338629,1790429033,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & 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Article  \nORIGINAL RESEARCH  \nSynthesis of Zinc Oxide-Doped Carbon Dots for Treatment of Triple-Negative Breast Cancer  \nMengqi Wang 1 , *, Shuting Lan 2 , *, Mingjun Song 3 , Rongrong Zhang 2 , Wenqi Zhang 2 , Xiaomei Sun 2 , Gang Liu 2  \n1College of Life Science and Oceanography, Weifang University, Weifang, Shandong, People’s Republic of China; 2Key Laboratory of Medical Cell Biology, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, People’s Republic of China; 3College of Chemical Engineering and Environmental Chemistry, Weifang University, Weifang, Shandong, People’s Republic of China  \n*These authors contributed equally to this work  \nCorrespondence: Mengqi Wang; Gang Liu, [Email wangmengqi@tju.edu.cn](Email wangmengqi@tju.edu.cn); [20190043@immu.edu.cn](20190043@immu.edu.cn)  \n\n| Introduction: The anti-cancer properties of zinc oxide-doped carbon dots (CDs/ZnO) in inhibiting triple-negative breast cancer (TNBC) progression merit more investigation.\u003Cbr>Methods: With citric acid as the carbon source, urea applied as the nitrogen source, and zinc oxide (ZnO) used as a reactive dopant, CDs/ZnO were synthesized by microwave heating in the current study, followed by the characterization and biocompatibility assessments. Subsequently, the anti-cancer capabilities of CDs/ZnO against TNBC progression were evaluated by various biochemical and molecular techniques, including viability, proliferation, migration, invasion, adhesion, clonogenicity, cell cycle distribution, apoptosis, redox homeostasis, metabolome, and transcriptome assays of MDA-MB-231 cells. Additionally, the in vivo anti-cancer potentials of CDs/ZnO against TNBC progression were analyzed using TNBC xenograft mouse models.\u003Cbr>Results: The biocompatibility of CDs/ZnO was supported by the non-significant changes in the pathological and physiological parameters in the CDs/ZnO treated mice, alongside a non-cytotoxic effect of CDs/ZnO on the proliferation of normal cells. Notably, the CDs/ZnO treatments effectively decreased the viability, proliferation, migration, invasion, adhesion, and clonogenicity of MDAMB-231 cells. Furthermore, the CDs/ZnO treatments induced cell cycle arrest, apoptosis, redox imbalance, metabolome disturbances, and transcriptomic alterations of MDA-MB-231 cells by regulating the MAPK signaling pathway. Additionally, the CDs/ZnO treatments markedly suppressed the in vivo tumor growth in the TNBC xenograft mouse models.\u003Cbr>Conclusion: In this study, we synthesized CDs/ZnO via microwave heating, using citric acid as the carbon source, urea as the nitrogen source, and ZnO as a reactive dopant. We confirmed the biosafety and potent anti-cancer efficacy of CDs/ZnO in inhibiting TNBC progression by disrupting malignant cell behaviors through modulation of the MAPK signaling pathway.\u003Cbr>Keywords: zinc, carbon dots, triple-negative breast cancer, MAPK signaling pathway |\n| --- |\n| Introduction\u003Cbr>As a consequence of population growth and aging, the future incidence of breast cancer is projected to increase by 40% to over 3 million newly diagnosed cases and 1 million estimated deaths annually by 2040, leading to the recent launch of the Global Breast Cancer Initiative by the World Health Organization (WHO) in 2021.1 Incorporation of oncogenic driver mutations, various non-genetic risk events (such as hereditary, high mammographic density, chest radiation, high body mass index, menopausal hormone abuse, alcohol consumption, and inadequate physical activities) are contributing to the rising rates of breast cancer.2,3 Although the survival rates of breast cancer patients have been improved by populationbased breast cancer screening programs and timely diagnostic alternatives, along with adequate implementations of multidisciplinary precision preventives and individualised cancer medications, the absolute disease burdens caused by local recurrences and invasive relap","cbCailcdAOBOL1Av","https://ap.wps.com/l/cbCailcdAOBOL1Av","pdf",19215778,23,"English","# Introduction\n## Study aims and background\n# Methods\n## Microwave synthesis and experimental design\n## In vitro assays\n## In vivo xenograft evaluation\n# Results\n## Biocompatibility and anti-cancer effects\n## Mechanistic findings via MAPK signaling\n## In vivo tumor suppression\n# Conclusion\n## Summary of synthesis approach and efficacy","[{\"question\":\"How were zinc oxide-doped carbon dots (CDs/ZnO) synthesized?\",\"answer\":\"CDs/ZnO were synthesized by microwave heating using citric acid as the carbon source, urea as the nitrogen source, and zinc oxide (ZnO) as the reactive dopant.\"},{\"question\":\"What anti-cancer effects did CDs/ZnO show against TNBC cells?\",\"answer\":\"CDs/ZnO decreased viability, proliferation, migration, invasion, adhesion, and clonogenicity of MDA-MB-231 cells and induced cell-cycle arrest and apoptosis.\"},{\"question\":\"How did the study relate CDs/ZnO effects to molecular mechanisms and in vivo outcomes?\",\"answer\":\"The treatments disrupted redox homeostasis, metabolome, and transcriptomic profiles by regulating the MAPK signaling pathway, and they markedly suppressed tumor growth in TNBC xenograft mouse models.\"}]","Synthesis of Zinc Oxide-Doped Carbon Dots for Treatment of Triple-Negative Breast Cancer | PDF",1790024679,58]