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This study evaluated whether combining ellagic acid and Kaempferol enhances antitumor activity in vitro and in vivo using breast cancer models. In vitro assays assessed antiproliferative effects across five cancer cell lines and measured caspase-3 activity in T47-D cells with MTT readouts. In vivo testing in Balb/C mice examined tumor volume, tumor-free proportions, and safety through liver and kidney toxicity evaluation. The combination produced synergistic antiproliferative effects, stronger apoptosis induction, greater tumor reduction, and no detectable hepatic or renal toxicity, supporting further development as a breast cancer treatment candidate.",{"@graph":69,"@context":121},[70,84,104],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/synergistic-anticancer-effect-of-ellagic-acid-and-kaempferol-combination-in-a-mouse-model-of-breast-cancer/345560/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":98,"encodingFormat":97,"isAccessibleForFree":99,"interactionStatistic":100},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/synergistic-anticancer-effect-of-ellagic-acid-and-kaempferol-combination-in-a-mouse-model-of-breast-cancer/345560.png","ImageObject",300,407,{"name":92,"@type":93},"Miles","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-22",true,{"@type":101,"interactionType":102,"userInteractionCount":8},"InteractionCounter",{"@type":103},"ViewAction",{"@type":105,"mainEntity":106},"FAQPage",[107,113,117],{"name":108,"@type":109,"acceptedAnswer":110},"What was the main objective of the study?","Question",{"text":111,"@type":112},"To investigate the antitumor activity of combining ellagic acid and Kaempferol in breast cancer models, assessing both efficacy and safety.","Answer",{"name":114,"@type":109,"acceptedAnswer":115},"How were the treatments evaluated in vitro?",{"text":116,"@type":112},"Ellagic acid, Kaempferol, and their combination were tested against multiple cancer cell lines, with antiproliferative effects measured using the MTT assay and apoptosis-related caspase-3 activity measured in T47-D cells.",{"name":118,"@type":109,"acceptedAnswer":119},"What key outcomes were observed in vivo regarding safety and tumor control?",{"text":120,"@type":112},"The combination notably reduced tumor volume, increased the proportion of tumor-free mice, and showed no signs of liver or kidney toxicity in the combination-treated group.","https://schema.org",{"og:url":83,"og:type":123,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":125,"canonical":83},"index,follow",{"doc_id":127,"site_id":62},345560,1790096107,{"code":4,"msg":5,"data":130},{"doc_id":127,"user_id":131,"nickname":92,"user_avatar":132,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":133,"file_id":134,"file_url":135,"file_type":136,"file_size":137,"view_count":8,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":138,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":36},13056703019404,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","TYPE Original Research PUBLISHED 28 May 2026  \nDOI 10.3389/fonc.2026.1754560  \nOPEN ACCESS  \nEDITED BY  \nNunzio Antonio Cacciola,  \nUniversity of Naples Federico II, Italy  \nREVIEWED BY  \nBairagi C. Mallick,  \nCentral University of Jharkhand, India Thi Phuong Nhung Tran,  \nIndustrial University of Ho Chi Minh City, Vietnam  \nSelçuk Gümüş,  \nBartin University, Türkiye  \n*CORRESPONDENCE  \nWamidh H. Talib  \n[w_talib@asu.edu.jo](w_talib@asu.edu.jo)  \nRECEIVED 27 November 2025  \nREVISED 27 April 2026  \nACCEPTED 01 May 2026  \nPUBLISHED 28 May 2026  \nCITATION  \nShihab RJ, Alasmari MM, Alshaeri HK, Hock M, Talib WH and Ostojic S (2026) Synergistic anticancer effect of ellagic acid and Kaempferol combination in a mouse model of breast cancer.  \nFront. Oncol. 16:1754560 .  \ndoi: 10.3389/fonc.2026.1754560  \nCOPYRIGHT  \n© 2026 Shihab, Alasmari, Alshaeri, Hock, Talib and Ostojic. This is an open-access article distributed under the terms of the  \nCreative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.  \nSynergistic anticancer effect of ellagic acid and Kaempferol combination in a mouse model of breast cancer  \nRand Jabbar Shihab 1, Moudi M Alasmari 2,3, Heba K Alshaeri 4, M´arta Hock 5,6, Wamidh H. Talib 7* and Sergej Ostojic 5,6  \n1 Department of Clinical Pharmacy and Therapeutics, Faculty of Pharmacy, Applied Science Private University, Amman, Jordan, 2College of Medicine, King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Jeddah, Saudi Arabia, 3 King Abdullah International Medical Research Centre (KAIMRC), Jeddah, Saudi Arabia, 4 Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, Rabigh, Saudi Arabia, 5 Institute of Physiotherapy and Sports Science, Faculty of Health Sciences, University of Pe´ cs, Pe´cs, Hungary, 6 Physical Activity Research Group, J´anos Szent´agothai Research Center, University of Pe´ cs, Pe´cs, Hungary, 7 Faculty of Allied Medical Sciences, Applied Science Private University, Amman, Jordan  \nBackground: Cancer continues to be a major global health issue and is one of the leading causes of death. Breast cancer ranks among the most common neoplasms worldwide. Natural products have shown notable advantages in cancer treatment. Ellagic acid (EA), a polyphenolic compound found in natural sources, has potent anticancer properties. Kaempferol (KAE) is a natural substance known for its strong anticancer activity. This study investigated the antitumor activity of the combined use of EA and KAE on various cancer cell lines. Methods: In vitro, EA, KAE, and their combination were evaluated for their antiproliferative activity against five cancer cell lines: MDA-MB-231, T47-D, HeLa, EMT-6/P, A549, as well as the non-cancerous Vero cell line. The impact of each treatment on cell viability was examined using the MTT assay. T47-D cells were treated with EA, KAE, and a combination of both, and caspase-3 activity was measured. For the in vivo study, EMT-6/P cells were injected into Balb/C mice, which were treated with EA, KAE, and their combination. Tumor size and weight were measured, and hepatotoxicity and nephrotoxicity were also investigated for all treatments.  \nResults: In vitro, the combination of EA and KAE exerted a synergistic antiproliferative effect across all tested cancer cell lines, with the most pronounced effect observed in T47-D cells. This combination produced low toxicity in the non-cancerous Vero cell line. Furthermore, the combination led toa greater induction of apoptosis compared to single-agent treatment. In vivo, the EA and KAE combination exhibited a notable reduction in tumor volume (p\u003C 0.05) and increased proportion of tumor-free mice. Safety ev","cbCaierscwGZDyhZ","https://ap.wps.com/l/cbCaierscwGZDyhZ","pdf",1826300,16,"English","# Introduction\n## Materials and methods\n## Results\n## Conclusion","[{\"question\":\"What was the main objective of the study?\",\"answer\":\"To investigate the antitumor activity of combining ellagic acid and Kaempferol in breast cancer models, assessing both efficacy and safety.\"},{\"question\":\"How were the treatments evaluated in vitro?\",\"answer\":\"Ellagic acid, Kaempferol, and their combination were tested against multiple cancer cell lines, with antiproliferative effects measured using the MTT assay and apoptosis-related caspase-3 activity measured in T47-D cells.\"},{\"question\":\"What key outcomes were observed in vivo regarding safety and tumor control?\",\"answer\":\"The combination notably reduced tumor volume, increased the proportion of tumor-free mice, and showed no signs of liver or kidney toxicity in the combination-treated group.\"}]","Synergistic anticancer effect of ellagic acid and Kaempferol combination in a mouse model of breast cancer | PDF",1790058092]