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During breast biopsy collection and surgical intervention, on-site pathology guidance is not routinely available, leading to repeat biopsy and re-excision that increase cost and patient discomfort. Because existing on-site techniques are destructive and inaccurate, ex-vivo microscopy offers a non- or minimally processed alternative. This study tests structured illumination microscopy (SIM) on freshly obtained breast tissues to support rapid structure and cancer identification. Using a customized SIM system and a dual-color fluorescent H&E analog, 47 biopsies were imaged, yielding 96% binary diagnostic accuracy versus gold-standard histology and enabling identification of key tissue features including stroma, vessels, ducts, and lobules.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/structured-illumination-microscopy-for-cancer-identification-in-diagnostic-breast-biopsies-research-article/342769/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/structured-illumination-microscopy-for-cancer-identification-in-diagnostic-breast-biopsies-research-article/342769.png","ImageObject",300,407,{"name":92,"@type":93},"Eliana","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-23","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":8},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why is on-site pathology guidance during breast biopsy currently limited?","Question",{"text":112,"@type":113},"On-site pathology for location guidance is not commonly available during biopsy collection or surgical intervention. Existing clinical on-site evaluation techniques are destructive and inaccurate.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was structured illumination microscopy (SIM) evaluated in the study?",{"text":117,"@type":113},"The researchers imaged 47 diagnostic breast biopsies using a customized SIM system and a dual-color fluorescent hematoxylin & eosin (H&E) analog, then compared results to standard histopathology.",{"name":119,"@type":110,"acceptedAnswer":120},"What diagnostic performance did SIM achieve compared with gold-standard histology?",{"text":121,"@type":113},"A board-certified pathologist reported 96% binary diagnostic accuracy when comparing SIM-derived diagnoses with diagnoses from gold-standard histology slides.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},342769,1790194661,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":8,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},4398048949847,"https://ap-avatar.wpscdn.com/avatar/400002536579ef2da7f?_k=1778318612642679267","PLOS ONE  \nOPEN ACCESS  \nCitation: Behr M, Alizadeh L, Buckner-Baiamonte L, Roberts B, Sholl AB, Brown JQ (2024)  \nStructured illumination microscopy for cancer identification in diagnostic breast biopsies. PLoSONE 19(5): e0302600 . [https://doi.org/10.1371/](https://doi.org/10.1371/)[ ](https://doi.org/10.1371/)[journal.pone.0302600](journal.pone.0302600)  \nEditor: David Mayerich, University of Houston, UNITED STATES  \nReceived: November 27, 2023  \nAccepted: April 8, 2024  \nPublished: May 9, 2024  \nCopyright: © 2024 Behr et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.  \nData Availability Statement: Raw data for this study is stored in FigShare for public access. The pathologist interpretation data can be accessed with the DOI: 10.6084/m9 .figshare.25234585. The raw data for microscopy imaging speed for each biopsy can be accessed with the DOI: 10.6084/m9 . figshare.25234636.  \nFunding: Financial support for this work was provided by a grant from the National Cancer Institute of the National Institutes of Health, grant no. R01 CA222831 to JQB. Funders did not play  \nRESEARCH ARTICLE  \nStructured illumination microscopy for cancer identification in diagnostic breast biopsies  \nMadeline Behr1, Layla Alizadeh2, Lyndsey Buckner-Baiamonte3, Brett Roberts4, Andrew  \nB. Sholl5, J. Quincy Brown1 *  \n1 Department of Biomedical Engineering, Tulane University, New Orleans, LA, United States of America, 2 Department of Pathology, Ochsner Medical Center, New Orleans, LA, United States of America,  \n3 Biorepository Unit, Ochsner Medical Center, New Orleans, LA, United States of America, 4 Department of Radiology, Ochsner Medical Center, New Orleans, LA, United States of America, 5 Department of Pathology, Touro Infirmary, New Orleans, LA, United States of America  \n* [jqbrown@tulane.edu](jqbrown@tulane.edu)  \nAbstract  \nBreast cancer is the second most common cancer diagnosed in women in the US with almost 280,000 new cases anticipated in 2023. Currently, on-site pathology for location guidance is not available during the collection of breast biopsies or during surgical intervention procedures. This shortcoming contributes to repeat biopsy and re-excision procedures, increasing the cost and patient discomfort during the cancer management process. Both procedures could benefit from on-site feedback, but current clinical on-site evaluation techniques are not commonly used on breast tissue because they are destructive and inaccurate. Ex-vivo microscopy is an emerging field aimed at creating histology-analogous images from non-or minimally-processed tissues, and is a promising tool for addressing this pain point in clinical cancer management. We investigated the ability structured illumination microscopy (SIM) to generate images from freshly-obtained breast tissues for structure identification and cancer identification at a speed compatible with potential on-site clinical implementation. We imaged 47 biopsies from patients undergoing a guided breast biopsy procedure using a customized SIM system and a dual-color fluorescent hematoxylin & eosin (H&E) analog. These biopsies had an average size of 0.92 cm2 (minimum 0 . 1, maximum 4 .2) and had an average imaging time of 7:29 (minimum 0:22, maximum 37:44) . After imaging, breast biopsies were submitted for standard histopathological processing and review. A board-certified pathologist returned a binary diagnostic accuracy of 96% when compared to diagnoses from gold-standard histology slides, and key tissue features including stroma, vessels, ducts, and lobules were identified from the resulting images.  \nIntroduction  \nBreast cancer is the second most common cancer diagnosis in women, after skin cancer, with almost 280,000 new cases predicted in the US in 2023 [ 1] . Suspicion of breast cancer is typically ","cbCaiv4356i2esZP","https://ap.wps.com/l/cbCaiv4356i2esZP","pdf",1425833,14,"English","# Abstract\n# Introduction","[{\"question\":\"Why is on-site pathology guidance during breast biopsy currently limited?\",\"answer\":\"On-site pathology for location guidance is not commonly available during biopsy collection or surgical intervention. Existing clinical on-site evaluation techniques are destructive and inaccurate.\"},{\"question\":\"How was structured illumination microscopy (SIM) evaluated in the study?\",\"answer\":\"The researchers imaged 47 diagnostic breast biopsies using a customized SIM system and a dual-color fluorescent hematoxylin \\u0026 eosin (H\\u0026E) analog, then compared results to standard histopathology.\"},{\"question\":\"What diagnostic performance did SIM achieve compared with gold-standard histology?\",\"answer\":\"A board-certified pathologist reported 96% binary diagnostic accuracy when comparing SIM-derived diagnoses with diagnoses from gold-standard histology slides.\"}]","Structured illumination microscopy for cancer identification in diagnostic breast biopsies - Research article | PDF",1790048142,35]