[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-459757-105":59,"doc-detail-459757-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","solution-key-7013-finals-question-1","Solution Key - 7.013 Finals - Question 1","","Answer key covering biomedical genetics and cancer pharmacology questions from “7.013 Finals (5/19/09)”. It explains how familial adenomatous polyposis (FAP) relates to APC mutations, interprets gel electrophoresis results for APC DNA and protein across WBC and colon cell types, determines which individual develops colon cancer without FAP, and completes APC genotype tables. It also addresses how vincristine disrupts mitotic microtubules, compares candidate chemotherapeutic compounds by therapeutic index, and evaluates EPO mutations affecting RBC production. Includes a subsequent plant genetics question setup.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/solution-key-7013-finals-question-1/459757/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/solution-key-7013-finals-question-1/459757.png","ImageObject",300,407,{"name":92,"@type":93},"eBook King","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-31","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why is APC protein detected only in colon cells in the normal individual even when APC DNA is present in both tissues?","Question",{"text":112,"@type":113},"All somatic cells share the same DNA, but each cell type expresses only specific sets of genes. APC protein is therefore translated/detected only in colon cells due to cell-type-specific gene expression.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which individual would not have FAP yet still develop colon cancer, and how does it happen?",{"text":117,"@type":113},"Individual #2. The data indicate a sporadic case where spontaneous somatic mutation affects both APC alleles in colon cells, producing a non-functional APC protein that drives cancer development.",{"name":119,"@type":110,"acceptedAnswer":120},"How does vincristine prevent cancer cell growth?",{"text":121,"@type":113},"Vincristine inhibits microtubule assembly, disrupting spindle fiber formation during mitosis. Sister chromatids fail to separate properly, inhibiting cell division and making cells non-viable, thereby blocking tumor growth.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},459757,1790909209,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},962088006270,"https://ap-avatar.wpscdn.com/davatar_085a072bc5b1113ac321206ff7593b45","Solution Key-7.013 Finals (5 / 19 / 09)  \nQuestion 1  \nFamilial adenomatous polyposis (FAP) affects nearly 1/8000 people in the USA. Patients having FAPare genetically predisposed to colon cancer. Mutations in the APC gene have been identified as the probable cause of FAP.  \n(a) The following diagram represents the gel electrophoretic profiles of both the PCR amplified APC DNA (top panel) and APC protein (bottom panel) isolated from white blood cells (WBCs) and colon cancer cells of two individual patients. (A profile of the APC DNA and APC protein in a normal individual is provided as a reference. Please note the intensity of the bands while answering this question).  \nTop  panel  \nBottom panel   \nIndividuals  \nCell types  \nPCR amplified APC DNA  \nCell types  \nAPC protein  \n\n| Normal |  | \\#1 |  | \\#2 |  |\n| --- | --- | --- | --- | --- | --- |\n| WBC | Colon | WBC | Cancer | WBC | Cancer |\n| | |  | | | |\n| WBC | Colon | WBC | Cancer | WBC | Cancer |\n|  | |  | |  | |\n\nMW  \nHigh  \nLow  \nHigh  \nLow  \ni. Explain why in the normal individual, the APC protein is detected only in colon cells even though the APC DNA is present in both colon cells and WBCs.  \nAll somatic cells in an individual have the same DNA and hence the same set of genes. However, each cell type in an individual expresses only specific set of genes which regulate their shape, size and functions.  \nii. One of these two individuals does not have FAP but still develops colon cancer. Given the data above, which individual would this be? Explain how this individual got colon cancer.  \nIndividual \\#2 does not have FAP but sporadically develops colon cancer. This individual undergoes a spontaneous somatic mutation of both alleles of APC genes in colon cells producing a non-functional APC protein that leads to colon cancer.  \niii. Complete the following table based on the information provided in the gel profile above.(Use the symbols‘+’ to represent the wild-type allele of the APC gene, ‘-‘ to represent the loss of function mutation and‘M’ to represent the gain of function mutation. The genotype of the APC gene in a normal individual is provided as a reference).  \n\n| Individuals | Genotype of APC gene |  | Is the genotype of WBC different from colon cancer cells? If yes, explain why. |\n| --- | --- | --- | --- |\n|  | WBC | Colon cells or\u003Cbr>Colon cancer cells |  |\n| Normal | +/+ | +/+ |  |\n| \\#1 | +/ - | -/- | Yes, APC gene shows loss of heterozygosity |\n| \\#2 | +/+ | -/ - | -do- |\n\nQuestion 1 continued  \n(b) Vincristine is an inhibitor of microtubule assembly and is used as an important chemotherapeutic drug. Explain how the disruption of microtubule assembly may prevent cancer cell growth.  \nMicrotubules are required for the formation of spindle fibers during cell division that are required to pull the sister chromatids towards the two poles. If these are disrupted the sister chromatids fail to separate properly thus inhbiting cell division and making the cell non-viable. Thus the growth of tumor will be inhibited.  \n(c) During drug screening you identify two compounds A and B that have the potential to kill colon cancer cells and normal cells as shown by the following graph.  \n100%  \n% cell alive  \n50%  \n\\#1: Cancer cells + Compound A  \n\\#2: Cancer cells + Compound B  \n\\#3: Normal cells +Compound B  \n\\#4: Normal cells + Compound A  \n\\#1 \\#2 \\#3 \\#4  \nCompound concentration   \nWhich of these two compounds is a better candidate for colon cancer treatment? Explain why.  \nCompound A will be a better choice since it has a larger therapeutic index i.e. The effective concentration of compound A that is required to kill the cancer cells is far less compared to the concentration needed to kill the normal cells.  \n(d) Many patients show signs of severe anemia as a side effect of chemotherapy and are prescribed erythropoietin (EPO). EPO is a secreted protein, produced by the kidney, which binds to its receptor onerythroid precursor cells and stimulates the formation of red blood c","cbCaiaWTlAy4RMKK","https://ap.wps.com/l/cbCaiaWTlAy4RMKK","pdf",678135,22,"English","# Question 1\n## (a)\n## (b)\n## (c)\n## (d)\n# Question 2","[{\"question\":\"Why is APC protein detected only in colon cells in the normal individual even when APC DNA is present in both tissues?\",\"answer\":\"All somatic cells share the same DNA, but each cell type expresses only specific sets of genes. APC protein is therefore translated/detected only in colon cells due to cell-type-specific gene expression.\"},{\"question\":\"Which individual would not have FAP yet still develop colon cancer, and how does it happen?\",\"answer\":\"Individual #2. The data indicate a sporadic case where spontaneous somatic mutation affects both APC alleles in colon cells, producing a non-functional APC protein that drives cancer development.\"},{\"question\":\"How does vincristine prevent cancer cell growth?\",\"answer\":\"Vincristine inhibits microtubule assembly, disrupting spindle fiber formation during mitosis. Sister chromatids fail to separate properly, inhibiting cell division and making cells non-viable, thereby blocking tumor growth.\"}]","Solution Key - 7.013 Finals - Question 1 | PDF",1790756811,55]