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Public databases and an immunohistochemical assay assessed SMC4 expression in pan-cancer and COAD tissues. COAD cells were manipulated with si-RNA, followed by proliferation, viability, autophagy, migration, and EMT marker analyses. Findings show SMC4 upregulation, poor patient prognosis associations, and links to neutrophil, M2 macrophage, and CD4+ T-cell infiltration, suggesting biomarker potential.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/smc4-serves-as-a-potential-marker-for-the-diagnosis-and-prognosis-of-colon-adenocarcinoma/332632/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/smc4-serves-as-a-potential-marker-for-the-diagnosis-and-prognosis-of-colon-adenocarcinoma/332632.png","ImageObject",300,407,{"name":92,"@type":93},"River Wang","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-04","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main purpose of studying SMC4 in colon adenocarcinoma?","Question",{"text":112,"@type":113},"To explore how SMC4 relates to malignant progression and the immune landscape of COAD, and whether it can serve as a diagnosis and prognostic marker.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was SMC4 function tested in COAD cells?",{"text":117,"@type":113},"SMC4 protein levels were screened in COAD tissues by immunohistochemistry, and COAD cells were transfected with si-RNA; then proliferation/viability, autophagy, migration, and EMT-related changes were evaluated using assays described in the methods.",{"name":119,"@type":110,"acceptedAnswer":120},"What do the results indicate about SMC4 in patient prognosis?",{"text":121,"@type":113},"SMC4 was upregulated and correlated with poor prognosis in COAD, with additional associations involving TMB and MSI as reported in the results.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},332632,1790179787,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},1099514067438,"https://ap-avatar.wpscdn.com/avatar/100002539ee87300030?x-image-process=image/resize,m_fixed,w_180,h_180&k=1780474512215547542","Original Research Article  \n| SMC4 serves as a potential marker for the diagnosis and prognosis of colon adenocarcinoma | International Journal of Immunopathology and Pharmacology Volume 38: 1–14\u003Cbr>© The Author(s) 2024\u003Cbr>Article reuse guidelines:\u003Cbr>[sagepub.com/journals-permissions](sagepub.com/journals-permissions)\u003Cbr>[DOI: 10.1177/03946320241286565](DOI: 10.1177/03946320241286565)\u003Cbr>[journals.sagepub.com/home/iji](journals.sagepub.com/home/iji)\u003Cbr>\u003Cbr>|\n| --- | --- |\n| Dawei Yang 1,*, Wenxin Cheng2,*, Ying Liu3, Liang Ma 1, Yao Sun 1, Hongzhen Wang4, Haifeng Liu 1, Li Nan5, Yang Yang3 and Xinyue Wang3,6 􀀁\u003Cbr>Abstract\u003Cbr>Objective: We aimed to explore the role of structural maintenance of chromosomes 4 (SMC4) in malignant progression and immunology of colon adenocarcinoma (COAD).\u003Cbr>Methods: The expression, genetic and protein features, and immune cell infiltration of SMC4 in pan-cancer were provided by public databases and websites. The protein expression of SMC4 in COAD tissues was screened by immunohistochemical assay. Si-RNA-mediated transfection was performed in COAD cells and the proliferation viability was measured using MTT, colony formation and EdU assays. Cell autophagy was detected by AO staining, western blots, and immunofluorescence staining. The migratory ability was determined using scratch and transwell assays. The expression of epithelial-to-mesenchymal transition (EMT) markers and transcriptional factors were detected using western blots. Results: The expression of SMC4 was upregulated in pan-cancer and had relationships with prognosis, TMB, and MSI of cancer patients. Particularly, SMC4 protein was highly expressed in COAD tissues and correlated with poor prognosis of patients. Depletion of SMC4 inhibited cell proliferation, induced autophagy, and decreased migration through EMT progression in COAD cells. In addition, SMC4 was associated with infiltration of neutrophils, M2 macrophages, and CD4 + T cells in COAD, and had positive association with M2 macrophage markers and immune checkpoints.\u003Cbr>Conclusion: SMC4 was correlated with patients’ poor prognosis, proliferation, metastasis, and immune cell infiltrates, and might function as a potential diagnosis and prognostic biomarker in COAD.\u003Cbr>Keywords\u003Cbr>structural maintenance of chromosomes 4, colon adenocarcinoma, epithelial-to-mesenchymal transition, immune infiltration, biomarker\u003Cbr>Date received: 7 February 2024; accepted: 27 August 2024 |  |\n| 1 Department of General Surgery, Siping Central People’s Hospital, Siping, Jilin, China 2Department of Surgery, Chinese Medical Sciences University, Shenyang, Liaoning, China\u003Cbr>3Key Laboratory of Pathobiology (Yanbian University), State Ethnic Affairs Commission, Yanji, Jilin, China 4Life Science Academy, Jilin Normal University, Siping, China\u003Cbr>5Institute of Virology, Wenzhou University, Wenzhou, China\u003Cbr>6Postdoctoral Research Workstation, Jilin Cancer Hospital, Changchun, Jilin, China\u003Cbr>*These authors contributed equally to this work and should be considered co-first authors.\u003Cbr>Corresponding authors:\u003Cbr>Yang Yang, Key Laboratory of Pathobiology (Yanbian University), State Ethnic Affairs Commission, 977 Gongyuan Road, Yanji, Jilin 133000, P.R. China.\u003Cbr>Email: [yangyang@ybu.edu.cn](yangyang@ybu.edu.cn)\u003Cbr>Xinyue Wang, Key Laboratory of Pathobiology (Yanbian University), State Ethnic Affairs Commission, 977 Gongyuan Road, Yanji, Jilin 133000, and Postdoctoral Research Workstation, Jilin Cancer Hospital, 1066 Jinhu Road, High-tech Zone, Changchun, Jilin 133000, P.R. China.\u003Cbr>Email: [1090693307@qq.com](1090693307@qq.com) |  |\n\nCreative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons  \nAttribution-NonCommercial 4.0 License ([https://creativecommons.org/licenses/by-nc/4.0/](https://creativecommons.org/licenses/by-nc/4.0/)) which permits non-commercial use,  \nreproduction and distribution of the work without further permission provided the original work is attrib","cbCaigLciF3ygbbE","https://ap.wps.com/l/cbCaigLciF3ygbbE","pdf",3385032,14,"English","# Abstract\n## Objective\n## Methods\n## Results\n## Conclusion\n# Introduction","[{\"question\":\"What is the main purpose of studying SMC4 in colon adenocarcinoma?\",\"answer\":\"To explore how SMC4 relates to malignant progression and the immune landscape of COAD, and whether it can serve as a diagnosis and prognostic marker.\"},{\"question\":\"How was SMC4 function tested in COAD cells?\",\"answer\":\"SMC4 protein levels were screened in COAD tissues by immunohistochemistry, and COAD cells were transfected with si-RNA; then proliferation/viability, autophagy, migration, and EMT-related changes were evaluated using assays described in the methods.\"},{\"question\":\"What do the results indicate about SMC4 in patient prognosis?\",\"answer\":\"SMC4 was upregulated and correlated with poor prognosis in COAD, with additional associations involving TMB and MSI as reported in the results.\"}]","SMC4 serves as a potential marker for the diagnosis and prognosis of colon adenocarcinoma | PDF",1789998964,35]