[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-371177-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-371177-en":131},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","single-cell-transcriptomics-unveils-multifaceted-immune-heterogeneity-in-early-onset-versus-late-onset-cervical-cancer","Single-cell transcriptomics unveils multifaceted immune heterogeneity in early-onset versus late-onset cervical cancer","","Early-onset (EOCC) and late-onset cervical cancers (LOCC) represent two clinically distinct subtypes with different manifestations and treatment responses, yet their immune landscapes remain insufficiently characterized. Single-cell transcriptomic analysis of 4 EOCC and 4 LOCC samples compares immune architectures. EOCC epithelial cells show dual immune properties with CXCL-driven immunosuppression and HLA-driven stimulation. LOCC T cells display enhanced activation, NK cells show reduced cytotoxicity, macrophages polarize toward M1/M2, dendritic cells improve antigen presentation, and CAFs differ in inflammatory versus antigen-presenting enrichment, supporting age-tailored immunotherapy.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/single-cell-transcriptomics-unveils-multifaceted-immune-heterogeneity-in-early-onset-versus-late-onset-cervical-cancer/371177/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/single-cell-transcriptomics-unveils-multifaceted-immune-heterogeneity-in-early-onset-versus-late-onset-cervical-cancer/371177.png","ImageObject",300,407,{"name":42,"@type":43},"Patrick","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-01","2026-09-23",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",11,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What clinical distinction does the study focus on for cervical cancer?","Question",{"text":63,"@type":64},"The study compares early-onset cervical cancer (EOCC) and late-onset cervical cancer (LOCC), which are described as two clinically distinct subtypes with different manifestations and therapeutic responses.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How does EOCC differ from LOCC in epithelial and T/NK cell immune features?",{"text":68,"@type":64},"EOCC epithelial cells show immune-suppressive characteristics driven by elevated CXCL production alongside immune-stimulatory features linked to higher HLA expression. In LOCC, CD4+ and CD8+ T cells show a heightened activation state, while NK cells exhibit diminished cytotoxicity.",{"name":70,"@type":61,"acceptedAnswer":71},"What immune cell and CAF differences are reported between EOCC and LOCC?",{"text":72,"@type":64},"Macrophages in LOCC display enhanced polarization toward both M1 and M2 phenotypes, and dendritic cells show increased antigen-presenting capacity. For cancer-associated fibroblasts (CAFs), EOCC is enriched with inflammatory CAFs, whereas LOCC has a higher proportion of antigen-presenting CAFs.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},371177,1790729559,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,120,123,127],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},8,"Research & Report",30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":124,"doc_module":4,"doc_module_name":25,"category_name":125,"show_sort_weight":124,"slug":126},10,"Lifestyle","lifestyle",{"id":128,"doc_module":4,"doc_module_name":25,"category_name":129,"show_sort_weight":97,"slug":130},19,"General","general",{"code":4,"msg":82,"data":132},{"doc_id":79,"user_id":133,"nickname":42,"user_avatar":134,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":140,"language":141,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":12,"update_tm":145,"read_time":146},549758146520,"https://ap-avatar.wpscdn.com/avatar/80002397d8c0411e94?_k=1775819394049821470","Chen et al. World Journal of Surgical Oncology (2025) 23:12  \n[https://doi.org/10.1186/s12957-025-03654-z](https://doi.org/10.1186/s12957-025-03654-z)  \nWorld Journal of Surgical Oncology  \nRESEARCH Open Access  \nSingle-cell transcriptomics unveils   \nmultifaceted immune heterogeneity in earlyonset versus late-onset cervical cancer  \nQian Chen 1,2, Dongfeng Deng3, Hong Zhu 1,2 and Shan Li 1,2,4*  \nAbstract  \nEarly-onset (EOCC) and late-onset cervical cancers (LOCC) represent two clinically distinct subtypes, each defined by unique clinical manifestations and therapeutic responses. However, their immunological profiles remain poorly explored. Herein, we analyzed single-cell transcriptomic data from 4 EOCC and 4 LOCC samples to compare their immune architectures. Epithelial cell s in EOCC exhibited a notable dual immunological phenotype, characterized by immune-suppressive properties driven by elevated CXCL production, alongside immune-stimulatory features linked to heightened HLA molecule expression. CD4 +and CD8 + T cells in LOCC demonstrated a heightened activation state, while NK cells exhibited diminished cytotoxicity. Macrophages in LOCC displayed enhanced polarization towards both M1 and M2 phenotypes, along with dendritic cells showing augmented antigenpresenting capacity. Regarding cancer-associated fibroblasts (CAFs), EOCC was enriched with inflammatory CAFs, whereas LOCC harbored a higher proportion of antigen-presenting CAFs. These findings reveal the multifaceted immune heterogeneity between EOCC and LOCC, underscoring the imperative for age-tailored immunotherapeutic strategies.  \nKeywords Cervical cancer, Age, Single-cell RNA sequencing, Immune heterogeneity  \nIntroduction  \nCervical cancer remains a prevalent malignancy among women globally, with an estimated 660,000 new cases and 350,000 deaths reported annually [1]. Despite widespread screening initiatives and the availability of prophylactic vaccines targeting high-risk human papillomavirus  \n*Correspondence:  \nShan Li  \n[lishan2016@csu.edu.cn](lishan2016@csu.edu.cn)  \n1Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan, China  \n2National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China 3Department of Oncology, Hunan University of Medicine General Hospital, Huaihua, China  \n4Hunan Key Laboratory of Molecular Precision Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China  \n(HPV), cervical cancer persists as a significant public health challenge [2]. Precisely stratifying patients and customizing therapeutic approaches based on their distinct characteristics are pivotal for enhancing survival outcomes in cervical cancer [3, 4]. Among these characteristics, age has been recognized as a key factor influencing both clinical presentation and therapeutic response [5]. Younger cervical cancer patients often exhibit more aggressive histological subtypes, such as adenocarcinoma, and typically have a poorer prognosis due to rapid disease progression and resistance to conventional therapies [6, 7]. Conversely, older patients generally present with more indolent tumor behavior, although diagnosis often occurs at a more advanced stage, attributed to the subtle and insidious onset of symptoms [8, 9]. Classifying patients into early-onset and late-onset groups has  \n© The Author(s) 2025. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this a","cbCaigowvrGV4Fv3","https://ap.wps.com/l/cbCaigowvrGV4Fv3","pdf",12846075,17,"English","# Abstract\n# Introduction","[{\"question\":\"What clinical distinction does the study focus on for cervical cancer?\",\"answer\":\"The study compares early-onset cervical cancer (EOCC) and late-onset cervical cancer (LOCC), which are described as two clinically distinct subtypes with different manifestations and therapeutic responses.\"},{\"question\":\"How does EOCC differ from LOCC in epithelial and T/NK cell immune features?\",\"answer\":\"EOCC epithelial cells show immune-suppressive characteristics driven by elevated CXCL production alongside immune-stimulatory features linked to higher HLA expression. In LOCC, CD4+ and CD8+ T cells show a heightened activation state, while NK cells exhibit diminished cytotoxicity.\"},{\"question\":\"What immune cell and CAF differences are reported between EOCC and LOCC?\",\"answer\":\"Macrophages in LOCC display enhanced polarization toward both M1 and M2 phenotypes, and dendritic cells show increased antigen-presenting capacity. For cancer-associated fibroblasts (CAFs), EOCC is enriched with inflammatory CAFs, whereas LOCC has a higher proportion of antigen-presenting CAFs.\"}]","Single-cell transcriptomics unveils multifaceted immune heterogeneity in early-onset versus late-onset cervical cancer | PDF",1790191470,43]