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A first single-cell transcriptomic atlas was generated from ovarian tissues of 110-week-old laying hens with or without ovarian cancer. The study defined 12 cellular lineages and tumor-associated immune changes, including increased CD4+ and CD8+ T cells and reduced macrophages. Opposing prognostic effects of dominant Cyto_CD8+ gene signatures were validated in human datasets, supporting cross-species conservation and a molecularly traceable model.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/single-cell-transcriptomics-of-chicken-ovarian-cancer-identifying-immune-subsets-with-prognostic-implications/351083/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/single-cell-transcriptomics-of-chicken-ovarian-cancer-identifying-immune-subsets-with-prognostic-implications/351083.png","ImageObject",300,407,{"name":92,"@type":93},"Theodore","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-23","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":8},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What problem does the study address about ovarian cancer models?","Question",{"text":112,"@type":113},"Current preclinical models do not accurately recapitulate ovarian cancer early pathogenesis, contributing to poor prognosis. The study targets this gap by using a single-cell atlas approach.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What immune-cell changes were observed in ovarian cancer samples from laying hens?",{"text":117,"@type":113},"Tumor-associated changes included increased CD4+ and CD8+ T cells and reduced macrophages in the ovarian cancer samples.",{"name":119,"@type":110,"acceptedAnswer":120},"How were prognostic roles of Cyto_CD8+ subsets assessed?",{"text":121,"@type":113},"Gene signatures from two dominant Cyto_CD8+ subsets showed opposing prognostic roles when validated against human ovarian cancer datasets (GSE18520, GSE26712, GSE49997).","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},351083,1790151471,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":8,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},7971461740886,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","105 (2026) 106582  \nContents lists available at ScienceDirect  \nPoultry Science  \njournal [homepage:](homepage: www.elsevier.com/locate/psj)[ www.elsevier.com/locate/psj](homepage: www.elsevier.com/locate/psj)  \n| Single-cell transcriptomics of chicken ovarian cancer identifying immune subsets with prognostic implications\u003Cbr>Guoqiang Zhu a,b,1, Susanna Chau Yi Wang c,1, Hongqin Yao a,b, Jiliang He a,b,\u003Cbr>Jiannan Zhang a,b , Mao Zhang d, Yajun Wang a,b,* \u003Cbr>a Key laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China b Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610065, PR China c University of New South Wales, School of Biological, Earth & Environmental Sciences, Sydney, 2052, Australia\u003Cbr>d Division of Vascular Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610072, China |  |  |\n| --- | --- | --- |\n| A R T I C L E I N F O |  | A B S T R A C T |\n| Keywords:\u003Cbr>Laying hen, Ovarian cancer model Single-cell transcriptomics |  | Ovarian cancer (OC) is a lethal gynecological malignancy with a low 5-year survival rate. The lack of preclinical models accurately capturing OC's early pathogenesis contributes to this poor prognosis. The domestic laying hen uniquely develops spontaneous OC, but its single-cell-level insights for pathogenesis have been lacking. Here, we present the first single-cell transcriptomic atlas of ovarian tissues from 110-week-old laying hens with or without OC. We annotated 12 core cellular lineages and characterized tumor-associated changes, including increased CD4+ and CD8+ T cells and reduced macrophages in OC samples. T cells sub-clustering revealed conserved cell subtype-specific pH homeostasis regulation profiles across species. Notably, gene signatures from the two dominant Cyto_CD8+ subsets exhibited opposing prognostic roles when validated against human OC datasets (GSE18520, GSE26712, GSE49997). This study confirms the laying hen as a valuable OC model by demonstrating cross-species conservation in cellular heterogeneity and oncogenic pathways, advancing it from a pathologically analogous system to a molecularly traceable tool. |\n\nIntroduction  \nEpithelial ovarian cancer remains the deadliest gynecological malignancy, with approximately 70% of patients diagnosed at advancedstage metastatic disease and a 5-year survival rate below 30%(Malvezzi et al., 2016; Webb and Jordan, 2024). Current preclinical models do not fully recapitulate this disease pathogenesis (Tsang, et al., 2022). Genetically engineered mouse models, such as MISRII-CRE-driven strains, and the chemically induced rat models, rarely develop spontaneous ovarian tumors (Lengyel, et al., 2014). Although 3D in vitro models effectively dissect cell-autonomous mechanisms, they lack the systemic tumor microenvironment essential for studying metastasis (Bradaric, et al., 2013).  \nThe domestic laying hens (Gallus gallus domesticus) serves as a valuable model for ovarian cancer research (Bernardo, et al., 2015; Barua and Bahr, 2022; Johnson and Giles, 2013). This unique non-human species spontaneously develops epithelial ovarian cancer, with  \nage-dependent incidence rates of 24% in 3.5-year-old hens and up to 60% in 4-year-old hens (Johnson and Giles, 2006, 2013). Critically, its reproductive biology aligns with Fathalla’s incessant ovulation hypothesis (Fathalla, 1971), mimicking the human reproductive risk profile through daily ovulation. Laying hen tumors recapitulate all major human histological subtypes, including serous, endometrioid, mucinous, and clear-cell carcinomas, and exhibit hallmark metastatic patterns such as intra-abdominal seeding and ascites accumulation (Barua, et al., 2009; Giles, et al., 2006;). This model enables investigation of","cbCairALlhjlokcu","https://ap.wps.com/l/cbCairALlhjlokcu","pdf",7086840,11,"English","# Abstract\n# Keywords\n# Introduction\n## Limitations of current preclinical models\n## Domestic laying hen as an ovarian cancer model\n## Translational and cross-species relevance","[{\"question\":\"What problem does the study address about ovarian cancer models?\",\"answer\":\"Current preclinical models do not accurately recapitulate ovarian cancer early pathogenesis, contributing to poor prognosis. The study targets this gap by using a single-cell atlas approach.\"},{\"question\":\"What immune-cell changes were observed in ovarian cancer samples from laying hens?\",\"answer\":\"Tumor-associated changes included increased CD4+ and CD8+ T cells and reduced macrophages in the ovarian cancer samples.\"},{\"question\":\"How were prognostic roles of Cyto_CD8+ subsets assessed?\",\"answer\":\"Gene signatures from two dominant Cyto_CD8+ subsets showed opposing prognostic roles when validated against human ovarian cancer datasets (GSE18520, GSE26712, GSE49997).\"}]","Single-cell transcriptomics of chicken ovarian cancer identifying immune subsets with prognostic implications | PDF",1790092548,28]