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The study explores CSCs-related genes in gastric cancer epithelial cells. Methods: mRNA expression profiles and single-cell sequencing data for gastric cancer were downloaded from public databases and analyzed. Results: WDR72 was identified as a CSCs-related gene, highly expressed in gastric cancer tissues and linked to poorer patient prognosis, with negative correlations to CD8+ T-cell infiltration and activated memory CD4+ T cells. PD-L1 decreased with high WDR72, and WDR72 related to IC50 values across small-molecule drugs. Conclusion: WDR72 represents a novel CSCs-related gene in gastric cancer epithelial cells, showing higher expression with elevated stemness scores.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/single-cell-sequencing-analysis-revealed-that-wdr72-was-a-novel-cancer-stem-cells-related-gene-in-gastric-cancer/328624/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/single-cell-sequencing-analysis-revealed-that-wdr72-was-a-novel-cancer-stem-cells-related-gene-in-gastric-cancer/328624.png","ImageObject",300,407,{"name":92,"@type":93},"Ezra","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-05","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What was the main goal of the study regarding gastric cancer?","Question",{"text":112,"@type":113},"To investigate CSCs-related genes in gastric cancer epithelial cells using mRNA expression and single-cell sequencing data from public databases.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How is WDR72 linked to gastric cancer outcomes?",{"text":117,"@type":113},"WDR72 was identified as a CSCs-related gene, highly expressed in gastric cancer tissues, and high WDR72 expression was associated with inferior patient prognosis.",{"name":119,"@type":110,"acceptedAnswer":120},"What immune and drug-response relationships were reported for WDR72?",{"text":121,"@type":113},"WDR72 showed significant negative correlations with CD8+ T-cell infiltration and activated memory CD4+ T cells, was associated with reduced PD-L1 expression in patients with high WDR72, and correlated with the IC50 of multiple small-molecule drugs.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},328624,1790188729,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},1099514068035,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","Heliyon 10 (2024) e35549  \nContents lists available at ScienceDirect  \nHeliyon  \njournal [homepage:](homepage: www.cell.com/heliyon)[ www.cell.com/heliyon](homepage: www.cell.com/heliyon)  \n| Research article\u003Cbr>Single-cell sequencing analysis revealed that WDR72 was a novel cancer stem cells related gene in gastric cancer |  |  | |\n| --- | --- | --- | --- |\n| Lei Zheng a, 1 , Jia Lub, 1 , Dalu Konga, * , Yang Zhana, **\u003Cbr>a Department of Colorectal Oncology, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Key Laboratory of Digestive Cancer, Tianjin, 300060, China\u003Cbr>b Department of Infection Management, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Key Laboratory of Digestive Cancer, Tianjin, 300060, China |  |  |  |\n| A R T I C L E I N F O |  | A B S T R A C T |  |\n| Keywords:\u003Cbr>Cancer stem cells Gastric cancer Single-cell sequencing WDR72\u003Cbr>Prognosis |  | Background: Cancer stem cells (CSCs) are pivotal in tumor resistance to chemotherapy and gastric cancer’s rapid proliferation and metastasis. We aimed to explore the CSCs-related genes in gastric cancer epithelial cells.\u003Cbr>Methods: The mRNA expression profile and single-cell sequencing data of gastric cancer were downloaded from the public database.\u003Cbr>Results: We identified WDR72 as a CSCs-related gene in gastric cancer epithelial cells. WDR72 was highly expressed in gastric cancer tissues, and high expression of WDR72 was associated with inferior prognosis of patients. WDR72 expression had a significant negative correlation with the infiltration of CD8 + T cells and activated memory CD4 + T cells. PD-L1 expression was significantly reduced in gastric cancer patients with high WDR72 expression. WDR72 was correlated with IC50 of multiple small-molecule drugs.\u003Cbr>Conclusion: We identified a novel CSCs-related gene in gastric cancer epithelial cells, WDR72, which was highly expressed in patients with high stemness scores. |  |\n\n1. Introduction  \nGastric cancer is the fifth most prevalent cancer and it is also the third biggest cause of cancer-related deaths worldwide each year [1]. It generally begins in the cells that produce mucus in the stomach’s inner lining and can spread to other body parts if left untreated [2]. Factors that increase the risk of developing gastric cancer include a family history of the disease, Helicobacter pylori infection, smoking, a diet high in salt, and certain genetic mutations [3,4]. Symptoms of gastric cancer include indigestion, heartburn, bloating,  \n* Corresponding author. Department of Colorectal Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Key Laboratory of Digestive Cancer, Huanhuxi Road, Hexi District, Tianjin, 300060, China.  \n** Corresponding author. Department of Colorectal Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Key Laboratory of Digestive Cancer, Huanhuxi Road, Hexi District, Tianjin, 300060, China.  \nE-mail addresses: [18622626115@163.com](18622626115@163.com) (D. Kong), [zhanyang@tmu.edu.cn](zhanyang@tmu.edu.cn) (Y. Zhan).  \n1 These authors contributed equally this work.  \n[https://doi.org/10.1016/j.heliyon.2024.e35549](https://doi.org/10.1016/j.heliyon.2024.e35549)  \nReceived 22 January 2024; Received in revised form 29 July 2024; Accepted 31 July 2024 Available online 31 July 2024  \n2405-8440/© 2024 The Authors. Published by Elsevier Ltd. This is an open access article","cbCaiddvvdD7FNex","https://ap.wps.com/l/cbCaiddvvdD7FNex","pdf",13815546,15,"English","# Introduction\n## Gastric cancer background and risk factors\n## Cancer stem cells and their roles in tumor behavior\n## Study rationale and overview","[{\"question\":\"What was the main goal of the study regarding gastric cancer?\",\"answer\":\"To investigate CSCs-related genes in gastric cancer epithelial cells using mRNA expression and single-cell sequencing data from public databases.\"},{\"question\":\"How is WDR72 linked to gastric cancer outcomes?\",\"answer\":\"WDR72 was identified as a CSCs-related gene, highly expressed in gastric cancer tissues, and high WDR72 expression was associated with inferior patient prognosis.\"},{\"question\":\"What immune and drug-response relationships were reported for WDR72?\",\"answer\":\"WDR72 showed significant negative correlations with CD8+ T-cell infiltration and activated memory CD4+ T cells, was associated with reduced PD-L1 expression in patients with high WDR72, and correlated with the IC50 of multiple small-molecule drugs.\"}]","Single-cell sequencing analysis revealed that WDR72 was a novel cancer stem cells related gene in gastric cancer | PDF",1789980444,38]