[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-detail-144452-en":3,"doc-seo-144452-105":30,"detail-sidebar-cat-0-en-105":91},{"code":4,"msg":5,"data":6},0,"success",{"doc_id":7,"user_id":8,"nickname":9,"user_avatar":10,"doc_module":4,"category_id":11,"category_name":12,"doc_title":13,"doc_description":14,"doc_content":15,"file_id":16,"file_url":17,"file_type":18,"file_size":19,"view_count":4,"is_deleted":4,"is_public":20,"is_downloadable":20,"audit_status":20,"page_count":21,"language":22,"language_code":23,"site_id":24,"html_lang":23,"table_of_contents":25,"faqs":26,"seo_title":27,"seo_description":14,"update_tm":28,"read_time":29},144452,687197207639,"Asher","https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd",7,"Healthcare","SIHLER'S STAINING TECHNIQUE IN THE STUDY OF TONGUE INNERVATION IN THE RAT -","Modified Sihler's staining technique is used to demonstrate neuromuscular organization and the nerve branches within the rat tongue, showing both motor and sensory innervation. The study visualizes hypoglossal nerve distributions across tongue muscles, traces glossopharyngeal sensory fibers to the posterior one-third, and illustrates the lingual nerve course and branching from the corpus to the apex linguae. The method is emphasized as valuable for complex reinnervation and detailed nerve-branching analysis.","# SIHLER'S STAINING TECHNIQUE IN THE STUDY OF TONGUEINNERVATION IN THE RAT\n\nSIHLER BOYAMA TEKNIGI ILE RAT DIL INNERVASYONUNUN INCELENMESI  \nMeltem BAHCELIOGLU,M.D.,Deniz ERDOGAN,M.D.Cigdem ELMAS,M.D.,Rabet GÖZiL,M.D.,Engin CALGÜNER,M.D.  \nGazi University,Faculty of Medicine,Departments of Anatomy and Histology-Embryology,Ankara,TurkeyGazi Medical Journal 2004;15:133-137  \n\n| ÖZET  \u003Cbr>Amac:Sinirleri ve dallanmalarm ayrintlt bir sekilde  \u003Cbr>gösteren Sihler boyama teknigiyle farkl kraniyal sinirlerle  \u003Cbr>innerve edilen dilin nnervasyonu gösterilmistir:Metod  \u003Cbr>Sican dilinin duyu ve motor sinir innervasyonunu göstermek  \u003Cbr>amacryla modifiye Sihler boyama teknigi kullanlmstir:  \u003Cbr>Bulgular:Dil kaslarinda n.hypoglossus'un dagulm  \u003Cbr>gösterildi.Dilin 1/3 arka kzsmna n.glossopharyngeus'un  \u003Cbr>duyu liflerinin daglm izlendi.Ayrica,n.lingualis ve  \u003Cbr>dallarmn corpus linguae'dan apex linguae'ya dogru olan  \u003Cbr>seyri ve dallanmasz gösterildi.Sonuclar:Uzun bir süredir  \u003Cbr>gözardt edilmis olan bu boyama tekniginin yapilardaki  \u003Cbr>karmasik sinir dallanmalarm ve reinnervasyon deneylerinin  \u003Cbr>sonuclarm göstermesi bakmndan önemli bir yöntem  \u003Cbr>olacagi kamsndayz.  \u003Cbr>Anahtar Kelimeler:Sihler BoyamaRat,Dil,Nervus  \u003Cbr>Lingualis,Nervus Hypoglossus,Nervus Glossopharyngeus.   |\n| --- |\n\n\n| ABSTRACT  \u003Cbr>Purpose:The tongue is a complex muscular organ  \u003Cbr>constituted of several intrinsic and extrinsic muscles,  \u003Cbr>inmervated by several cranial nerves.Methods:In this study,  \u003Cbr>the nerves and their branches in the tongue were  \u003Cbr>demonstrated by Sihler's stain technique.Results:The  \u003Cbr>distributions of the hypoglossal nerve in the muscles of the  \u003Cbr>tongue were visualized.The sensitive fiber contribution of the  \u003Cbr>glossopharyngeal nerve to the posterior one-third of the  \u003Cbr>tongue was followed.In addition,the course of the lingual  \u003Cbr>nerve and its branchings in the corpus towards the tip were  \u003Cbr>demonstrated.Conclusion:We think that this overlooked  \u003Cbr>stain technique should be considered when studying the  \u003Cbr>complex innervations of structures,especially in re-  \u003Cbr>innervation experiments.  \u003Cbr>Key Words:Sihler's Staining,Rat,Tongue,Muscles,  \u003Cbr>Lingual Nerve,Hypoglossal Nerve,Glossopharyngeal Nerve.   |\n| --- |\n\n## INTRODUCTION\n\nModified Sihler's staining technique is highly  \nuseful in demonstrating neuromuscularorganization in complex structures.Using thistechnique,Liu et al.successfully studied thedistribution of intramuscular nerve branches inskeletal muscles(4).Drake et al.,on the otherhand,similarly applied Sihler's stainingtechnique to define the exact branching pattern ofnerves in the posterior cricoarytenoid muscles ofcanines,and claimed it was valuable both inunderstanding laryngeal function and in newtherapies for dysfunction(6).Wu et al.processed27 human hemi-larynges with Sihler's stainingtechnique,and suggested that the communicating  \nIt has been 100 years since Charles Sihlerfirst introduced his method for staining nervespindles and more than 50 years since otherworkers reported modifications of this techniquefor identifying innervation in the kidney,uterusand ovary in humans and in the foot and leg inanimals(1-4).  \nSihler's stain renders specimens translucentwhile counterstaining the entire nerve supply;consequently,individual peripheral nervesbecome clearly visible in fine detail and traceablein their normal three-dimensional position (5).  \nnerves supply motor innervation to thethyroarytenoid muscle as a second source,andsensory innervation to the subglottic area andcricoarytenoid joint(7).  \nThere are two other techniques for studyingintramuscular nerve supply,microdissection andserial histological sections.Microdissection isuseful only in identification of the more proximalnerve branches as they enter the substance of themuscle.Tracing nerve branches deeper into amuscle is fairly difficult and differentiating finebranches from small blood vessels andconnecting tissues could well be problematic.Pr","cbCaig5BxjbqLBPG","https://ap.wps.com/l/cbCaig5BxjbqLBPG","pdf",3768997,1,5,"English","en",105,"# Introduction\n## Materials and Methods","[{\"question\":\"What does the modified Sihler's staining technique help demonstrate in this study?\",\"answer\":\"It demonstrates neuromuscular organization and the sensory and motor nerve supply in complex structures, specifically the nerve branches within the rat tongue.\"},{\"question\":\"How are motor innervations of the rat tongue identified?\",\"answer\":\"The study visualizes the distributions of the hypoglossal nerve in the tongue muscles.\"},{\"question\":\"Which sensory nerve contribution is traced to the posterior one-third of the tongue?\",\"answer\":\"The glossopharyngeal nerve's sensory fibers are followed to the posterior one-third.\"}]","SIHLER'S STAINING TECHNIQUE IN THE STUDY OF TONGUE INNERVATION IN THE RAT - | PDF",1787702732,13,{"code":4,"msg":31,"data":32},"ok",{"site_id":24,"language":23,"slug":33,"title":13,"keywords":34,"description":14,"schema_data":35,"social_meta":86,"head_meta":88,"extra_data":90,"updated_unix":28},"sihlers-staining-technique-in-the-study-of-tongue-innervation-in-the-rat","",{"@graph":36,"@context":85},[37,54,68],{"@type":38,"itemListElement":39},"BreadcrumbList",[40,44,48,51],{"item":41,"name":42,"@type":43,"position":20},"https://docshare.wps.com","Home","ListItem",{"item":45,"name":46,"@type":43,"position":47},"https://docshare.wps.com/document/","Document",2,{"item":49,"name":12,"@type":43,"position":50},"https://docshare.wps.com/document/healthcare/",3,{"item":52,"name":13,"@type":43,"position":53},"https://docshare.wps.com/document/sihlers-staining-technique-in-the-study-of-tongue-innervation-in-the-rat/144452/",4,{"url":52,"name":13,"@type":55,"author":56,"headline":13,"publisher":58,"fileFormat":61,"inLanguage":23,"description":14,"dateModified":62,"datePublished":62,"encodingFormat":61,"isAccessibleForFree":63,"interactionStatistic":64},"DigitalDocument",{"name":9,"@type":57},"Person",{"url":41,"name":59,"@type":60},"DocShare","Organization","application/pdf","2026-08-26",true,{"@type":65,"interactionType":66,"userInteractionCount":4},"InteractionCounter",{"@type":67},"ViewAction",{"@type":69,"mainEntity":70},"FAQPage",[71,77,81],{"name":72,"@type":73,"acceptedAnswer":74},"What does the modified Sihler's staining technique help demonstrate in this study?","Question",{"text":75,"@type":76},"It demonstrates neuromuscular organization and the sensory and motor nerve supply in complex structures, specifically the nerve branches within the rat tongue.","Answer",{"name":78,"@type":73,"acceptedAnswer":79},"How are motor innervations of the rat tongue identified?",{"text":80,"@type":76},"The study visualizes the distributions of the hypoglossal nerve in the tongue muscles.",{"name":82,"@type":73,"acceptedAnswer":83},"Which sensory nerve contribution is traced to the posterior one-third of the tongue?",{"text":84,"@type":76},"The glossopharyngeal nerve's sensory fibers are followed to the posterior one-third.","https://schema.org",{"og:url":52,"og:type":87,"og:title":13,"og:site_name":59,"og:description":14},"article",{"robots":89,"canonical":52},"index,follow",{"doc_id":7,"site_id":24},{"code":4,"msg":5,"data":92},[93,97,101,105,109,114,117,122,127,130,134],{"id":20,"doc_module":4,"doc_module_name":46,"category_name":94,"show_sort_weight":95,"slug":96},"Story & Novel",90,"story-novel",{"id":47,"doc_module":4,"doc_module_name":46,"category_name":98,"show_sort_weight":99,"slug":100},"Literature",80,"literature",{"id":53,"doc_module":4,"doc_module_name":46,"category_name":102,"show_sort_weight":103,"slug":104},"Exam",70,"exam",{"id":21,"doc_module":4,"doc_module_name":46,"category_name":106,"show_sort_weight":107,"slug":108},"Comic",60,"comic",{"id":110,"doc_module":4,"doc_module_name":46,"category_name":111,"show_sort_weight":112,"slug":113},6,"Technology",50,"technology",{"id":11,"doc_module":4,"doc_module_name":46,"category_name":12,"show_sort_weight":115,"slug":116},40,"healthcare",{"id":118,"doc_module":4,"doc_module_name":46,"category_name":119,"show_sort_weight":120,"slug":121},8,"Research & Report",30,"research-report",{"id":123,"doc_module":4,"doc_module_name":46,"category_name":124,"show_sort_weight":125,"slug":126},9,"Religion & Spirituality",20,"religion-spirituality",{"id":125,"doc_module":4,"doc_module_name":46,"category_name":128,"show_sort_weight":125,"slug":129},"World Cup","world-cup",{"id":131,"doc_module":4,"doc_module_name":46,"category_name":132,"show_sort_weight":131,"slug":133},10,"Lifestyle","lifestyle",{"id":135,"doc_module":4,"doc_module_name":46,"category_name":136,"show_sort_weight":21,"slug":137},19,"General","general"]