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The study addresses how injured tissue inflammation can impair MSC performance, potentially limiting tissue restoration strategies. Human UC-MSCs are challenged with lipopolysaccharide (LPS), and outcomes are measured via CCK-8, scratch assays, differentiation assessment, and qRT-PCR. LPS does not reduce viability but suppresses migration and differentiation, especially at 50 μg/ml, alongside increased NLRP3, TLR4, RelA/p65, caspase-1, and IL-1β.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/short-term-inflammatory-exposure-affects-umbilical-cord-derived-mesenchymal-stem-cells-migration-and-differentiation-through-modulation-of-nlrp3-inflammasome-expression/456544/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/short-term-inflammatory-exposure-affects-umbilical-cord-derived-mesenchymal-stem-cells-migration-and-differentiation-through-modulation-of-nlrp3-inflammasome-expression/456544.png","ImageObject",300,407,{"name":92,"@type":93},"RuangKosong","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-09","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":56},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What was the purpose of exposing hUC-MSCs to an inflammatory environment?","Question",{"text":112,"@type":113},"To determine how short-term LPS-induced inflammation affects hUC-MSC viability, migration, differentiation, and NLRP3 inflammasome-related gene expression.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Does short-term LPS exposure reduce hUC-MSC viability?",{"text":117,"@type":113},"No. Short-term LPS induction did not affect the viability of hUC-MSCs in the reported results.",{"name":119,"@type":110,"acceptedAnswer":120},"How does LPS influence migration and differentiation in hUC-MSCs?",{"text":121,"@type":113},"LPS reduces migration and differentiation capacity, particularly at 50 μg/ml for both time points, with associated changes in inflammasome pathway gene expression.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},456544,1790817251,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":56,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":39,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":46,"preview_page_count":4},962090883219,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","Short-Term Inflammatory Exposure Affects Umbilical Cord-derived Mesenchymal Stem Cells Migration and Differentiation Through Modulation of NLRP3 Inflammasome Expression  \nHelsy Junaidi 1,2†, Dewi Sukmawati 1,3􀀍†, Anoop Narayanan V 4, Jeanne A. Pawitan 1,3,5,6  \nand Sri Widia Jusman 7  \n1. Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia  \n2. Department of Molecular Biology, Faculty of Medicine, Universitas Gunadarma, Depok, Indonesia  \n3. Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia  \n4. Nitte (Deemed to be University), NGSM Institute of Pharmaceutical Sciences (NGSMIPS), Department of Pharmaceutics, Mangaluru, Karnataka, India-57501  \n5. Stem Cell and Tissue Engineering (SCTE) Research Cluster, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia  \n6. Integrated Service Unit of Stem Cell Medical Technology (IPT TK Sel Punca), Dr. Cipto Mangunkusumo General Hospital (RSCM), Jakarta, Indonesia  \n7. Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia † The first and the second authors have had equal contribution to this manuscript  \n* Corresponding author:  \nDewi Sukmawati, Ph.D., Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia  \nTel: +62 21 3146129  \nE-mail: [ds_histoui@outlook.com](ds_histoui@outlook.com), [dewi.sukmawati@ui.ac.id](dewi.sukmawati@ui.ac.id)[ ](dewi.sukmawati@ui.ac.id)[Received:](Received: 6 Sept 2024)[ 6 Sept 2024](Received: 6 Sept 2024)  \n[Accepted](Accepted: 19 May 2025)[: 19 May 2025](Accepted: 19 May 2025)  \nAbstract  \nBackground: An innovative approach for tissue restoration using Umbilical Cordderived Mesenchymal Stem Cells (UC-MSCs) is hindered by their poor survival rate due to the detrimental effects of the injured tissue microenvironment. Activation of NLRP3 inflammasome in an inflammatory environment which is followed by cellular impairment, has been reported. However, the expression of NLRP3 inflammasome in UC-MSCs in response to the inflammatory environment is not well understood. This study aims to investigate the impact of short-term exposure to an inflammatory environment induced by Lipopolysaccharide (LPS) on hUC-MSCs, focusing on cell viability, migration, differentiation, and the expression of NLRP3 inflammasome-related genes.  \nMethods: hUC-MSC were exposed to LPS at concentration of 10 and 50 μg/ml for 3 and 6 hr. Cell viability was assessed using CCK-8 assay, migration capacity was evaluated using a scratch test, and differentiation capacity and the expression of NLRP3 inflammasome-related genes were measured using qRT-PCR.  \nResults: Short-term LPS induction did not affect the viability of hUC-MSCs but reduced their migration and differentiation capacity, particularly at 50 μg/ml for both time points (p\u003C0.05) . The induction caused an increase in the mRNA levels of NLRP3, TLR- 4, and RelA/p65, which correlated with elevated expression of caspase-1 and IL-1β . Conclusion: Short-term exposure to LPS influences hUC-MSCs by upregulating NLRP3, TLR4/ReIA (p65), IL-1β, and caspase-1 mRNA levels, leading to impaired migration and differentiation ability. This study underscores the significant impact of short-term exposure to an inflammatory microenvironment on hUC-MSC, potentially compromising their migration and differentiation capacity through the NLRP3 pathway.  \nKeywords: Inflammation, UC-MSC, Cell migration, Differentiation, Lipopolysaccharide, NLRP3 inflammasome  \nTo cite this article: Junaidi H, Sukmawati D, Narayanan AV, Pawitan JA, Jusman SW. ShortTerm Inflammatory Exposure Affects Umbilical Cord-derived Mesenchymal Stem Cells Migration and Differentiation Through Modulation of NLRP3 Inflammasome Expression. Avicenna J Med Biotech 2025;17(3):208-215.  \nIntroduction  \nMesenchymal Stem Cells (MSC) possess superior properties","cbCaity7VyuncLpY","https://ap.wps.com/l/cbCaity7VyuncLpY","pdf",698047,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusion\n# Keywords\n# Introduction","[{\"question\":\"What was the purpose of exposing hUC-MSCs to an inflammatory environment?\",\"answer\":\"To determine how short-term LPS-induced inflammation affects hUC-MSC viability, migration, differentiation, and NLRP3 inflammasome-related gene expression.\"},{\"question\":\"Does short-term LPS exposure reduce hUC-MSC viability?\",\"answer\":\"No. Short-term LPS induction did not affect the viability of hUC-MSCs in the reported results.\"},{\"question\":\"How does LPS influence migration and differentiation in hUC-MSCs?\",\"answer\":\"LPS reduces migration and differentiation capacity, particularly at 50 μg/ml for both time points, with associated changes in inflammasome pathway gene expression.\"}]","Short-Term Inflammatory Exposure Affects Umbilical Cord-derived Mesenchymal Stem Cells Migration and Differentiation Through Modulation of NLRP3 Inflammasome Expression | PDF",1790746349]