[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-337027-105":59,"doc-detail-337027-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","serum-zonulin-and-colorectal-cancer-risk","Serum zonulin and colorectal cancer risk","","Intestinal permeability is linked to colorectal cancer (CRC) development, and zonulin regulates tight junction function, enabling bacterial translocation. An Italian case-control study analyzed 77 CRC cases, 72 intestinal adenoma, and 76 healthy controls, totaling 148 tumor-free subjects. Serum zonulin was measured by ELISA, while bacterial DNA load was estimated using blood 16S rRNA gene copies. Logistic regression adjusted for center, sex, age, and education showed higher zonulin associated with increased CRC risk. Highest versus lowest zonulin tertiles yielded an OR of 2.36 (95% CI 1.14–4.86). Zonulin levels were higher in CRC patients, supporting gut barrier dysfunction and bacterial translocation in colorectal carcinogenesis.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/serum-zonulin-and-colorectal-cancer-risk/337027/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/serum-zonulin-and-colorectal-cancer-risk/337027.png","ImageObject",300,407,{"name":92,"@type":93},"Quinn","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",13,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"How was serum zonulin measured in the study?","Question",{"text":113,"@type":114},"Serum zonulin levels were quantified using an ELISA kit.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"What statistical model was used to assess the association with CRC risk?",{"text":118,"@type":114},"Logistic regression models were used and adjusted for center, sex, age, and education.",{"name":120,"@type":111,"acceptedAnswer":121},"What did the study find about zonulin levels and CRC risk?",{"text":122,"@type":114},"Zonulin showed a positive association with CRC risk, with an OR of 2.36 (95% CI 1.14–4.86) for the highest versus lowest tertiles compared with tumor-free subjects.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},337027,1790378199,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":44,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},962075114765,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nSerum zonulin and colorectal cancer risk  \nMirko Marino1, Silvia Mignozzi2, Karin B. Michels3, Marcello Cintolo4, Roberto Penagini5,6, Giorgio Gargari1, Clorinda Ciafardini6, Monica Ferraroni2,7, Linia Patel2, Cristian Del Bo’1, Pierfrancesco Leone8, Aldo Airoldi9, Maurizio Vecchi5,6, Rossella Bonzi2, Barbara Oreggia8, Pietro Carnevali10, Marcello Vangeli9, Massimiliano Mutignani4, Simone Guglielmetti11, Patrizia Riso1􀀍, Carlo LaVecchia2 & Marta Rossi2􀀍  \nIntestinal permeability has been related to colorectal cancer (CRC) development. Zonulin, a protein able to regulate tight junction function and intestinal permeability, emerges as a promising marker to elucidate the contribution of bacterial translocation in CRC. An Italian case-control study included 77 CRC cases, 72 intestinal adenoma and 76 healthy controls (for a total of 148 tumor-free subjects), aged 20–85. Serum zonulin levels were quantified by ELISA kit and blood 16S rRNA gene copies by DNA extraction and polymerase chain reaction. We applied logistic regression models adjusted for center, sex, age and education. There was a positive association between zonulin and CRC risk. The odds ratio (OR) ofCRC for the highest versus lowest tertile of zonulin as compared to tumor-free subjects was 2.36 (95% confidence interval, 1.14–4.86). The ORs were similar in colon and rectal cancers. The OR of colon cancer for the highest versus lowest levels of both zonulin and 16S rRNA gene copies was 4.55.  \nCirculating levels of zonulin were higher in CRC patients compared to tumor-free controls supporting the hypothesis of an interplay of gut barrier dysfunction and bacterial translocation in colorectal carcinogenesis. Zonulin may interact with 16S rRNA gene copies and serve as a further biomarker in the evaluation ofCRC diagnosis.  \nColorectal cancer (CRC) is one of the leading causes of cancer-related mortality worldwide, with about 1.8 million new cases diagnosed and about 1 million deaths each year1.  \nIntestinal microbiome is associated with the development of CRC2,3, and microbial translocation from the gastrointestinal tract to the bloodstream has been suggested to be implicated, too2,4–7. Intestinal permeability, also known as “leaky gut syndrome,” is a measure of the function of the intestinal barrier, which is responsible for selecting which elements enter the bloodstream from the intestine8. Intestinal dysbiosis, an alteration in the composition and function of intestinal microbiota, has been associated with an increase in intestinal permeability and an increased risk of CRC9, 10. Moreover, intestinal permeability can exacerbate gastrointestinal chronic inflammation and inflammatory diseases, such as Crohn’s disease and ulcerative colitis, which are known risk factors for CRC11–13.  \nZonulin is the only human protein able to (reversibly) regulate intestinal permeability by modulating intercellular tight junctions and is responsible for their opening and closing, allowing for the transit of molecules and fluids in and out of the gut14. In physiological conditions, zonulin plays an important role in regulating paracellular exchanges (e.g. dietary factors), and in protecting the gut barrier from the translocation of microbial cells15, 16. In pathological conditions, zonulin has also been implicated in the development of several metabolic diseases, such as type 2 diabetes and obesity, which are also recognised risk factors for CRC12, 17, 18.  \n1Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Milan, Italy.  \n2Branch of Medical Statistics, Biometry and Epidemiology “G.A. Maccacaro”, Department of Clinical Sciences and Community Health, Department of Excellence 2023-2027, University of Milan, Milan, Italy. 3Department of Epidemiology, Fielding School of Public Health, University of California, Los Angeles, USA. 4Digestive and Interventional Endoscopy Unit, ASS","cbCaingNHcfu68Ce","https://ap.wps.com/l/cbCaingNHcfu68Ce","pdf",1738547,"English","# Serum zonulin and colorectal cancer risk\n## Rationale: intestinal permeability, dysbiosis, and CRC\n## Zonulin as a regulator of tight junctions\n## Study aim and design\n## Measurement methods\n## Statistical analysis and results\n## Biological interpretation and implications","[{\"question\":\"How was serum zonulin measured in the study?\",\"answer\":\"Serum zonulin levels were quantified using an ELISA kit.\"},{\"question\":\"What statistical model was used to assess the association with CRC risk?\",\"answer\":\"Logistic regression models were used and adjusted for center, sex, age, and education.\"},{\"question\":\"What did the study find about zonulin levels and CRC risk?\",\"answer\":\"Zonulin showed a positive association with CRC risk, with an OR of 2.36 (95% CI 1.14–4.86) for the highest versus lowest tertiles compared with tumor-free subjects.\"}]","Serum zonulin and colorectal cancer risk | PDF",1790017435,23]