[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-detail-143079-en":3,"doc-seo-143079-105":30,"detail-sidebar-cat-0-en-105":92},{"code":4,"msg":5,"data":6},0,"success",{"doc_id":7,"user_id":8,"nickname":9,"user_avatar":10,"doc_module":4,"category_id":11,"category_name":12,"doc_title":13,"doc_description":14,"doc_content":15,"file_id":16,"file_url":17,"file_type":18,"file_size":19,"view_count":20,"is_deleted":4,"is_public":20,"is_downloadable":20,"audit_status":20,"page_count":21,"language":22,"language_code":23,"site_id":24,"html_lang":23,"table_of_contents":25,"faqs":26,"seo_title":27,"seo_description":14,"update_tm":28,"read_time":29},143079,962085662650,"Dozel","https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0",7,"Healthcare","Selective Gray and White Matter Staining of the Horse Spinal Cord - Research Article","Gray-to-white matter differentiation is a key clinical parameter for diagnosing diffuse and compressive spinal cord disorders, yet histological determination is hindered by difficulties related to tissue size and processing. This study evaluated staining and processing modifications using nine C6 segment samples from a female Shetland pony. Vacuum-assisted dehydration improved inadequate tissue dehydration, and six staining protocols were compared. Modified May-Grunwald-Giemsa (MMGG) produced the best results for gray and white matter discrimination, confirmed by blind macroscopic and microscopic scoring.","Kafkas Univ Vet Fak Derg 18 (2): 249-254, 2012  \nDOI:10.9775/kvfd.2011.5371  \nRESEARCH ARTICLE  \nSelective Gray and White Matter Staining of the Horse Spinal Cord  \nDurmus BOLAT * 􀀇  \nMuhammet L SELCUK **  \nSadullah BAHAR **  \nSadettin TIPIRDAMAZ **  \nEmrah SUR ***  \n* Kirikkale University, Faculty of Veterinary Medicine, Department of Anatomy, Campus, TR-71451Yahsihan, Kirikkale-TURKEY  \n** Selcuk University, Faculty of Veterinary Medicine, Department of Anatomy, Campus, TR-42000 Selcuklu, Konya-TURKEY  \n*** Selcuk University, Faculty of Veterinary Medicine, Department of Histology and Embryology, Campus, TR-42000 Selcuklu, Konya-TURKEY  \n Makale Kodu (Article Code): KVFD-2011-5371   \nSummary  \nThe ratio of gray and white matter is an important clinical parameter in the diagnosis of diffuse and compressive diseases of the spinal cord. Although histological methods are used to determine this parameter, there are some difficulties encountered in histological studies related to tissue size. The aim of this study was to evaluate possible modifications to overcome these difficulties. In the study, nine tissue samples taken from the C6 segment of a female Shetland pony and selected by systematic random sampling were used. The dehydration process of the spinal cord of the horse was supported by applying a vacuum. Paraffin blocks were prepared and cut into 10 µm sections to be stained separately with the different staining methods. Six different staining methods, including Modified May-Grunwald-Giemsa (MMGG), were compared and used to image entire slides. The stains, Hematoxylin & eosin (H&E), May-Grunwald-Giemsa (MGG), Masson’s trichrome (MT), AgNORs, Kluver Barrera (KB) and MMGG, were evaluated macroscopically and microscopically by participants who were unaware of which staining methods had been used. The staining methods were scored from worst (1) to best (5) using a Likert scale. Vacuum application was found to reduce the difficulties related to inadequate tissue dehydration. MMGG was selected as the best staining method in differentiating gray and white matter in the spinal cord of the horse.  \nKeywords: Gray matter ratio, Horse, Imaging, Spinal cord, Stain comparison  \nAt Omuriliğinin Gri ve Ak Maddesinin Seçici Boyanması  \nÖzet  \nMedulla spinalis’te yer alan gri ve ak madde oranları diffuz ve kompresif omurilik hastalıklarının teşhisinde önemli klinik parametrelerdendir. Bu parametrelerin tespitinde histolojik metotlar kullanılmasına rağmen, histolojik çalışmalarda doku büyüklüğüne bağlı bazı güçlüklüklerle karşılaşılmaktadır. Bu çalışmanın amacı, bahsedilen zorlukları aşmaya yönelik olası modifikasyonları değerlendirmektir. Çalışmada, Shetland pony ırkına ait C6 segmentinden sistematik rastgele örnekleme kuralına sadık kalınarak eldeedilen 9 doku kesiti kullanıldı . Medulla spinalis’in dehidrasyon işlemi sırasında vakum uygulaması yapıldı . Dokuların parafin blokları hazırlandı, dokular 10 µm kalınlığında kesilerek farklı histolojik boyalar ile boyandı . Modifiye May-Grunwald-Giemsa (MMGG)’nınyer aldığı 6 farklı boyama metodunun boyama performansları karşılaştırıldı . Hematoxylin & eosin (H&E), May-Grunwald-Giemsa (MGG), Masson’s trichrome (MT), AgNORs, Kluver Barrera (KB) ve MMGG ile boyanan kesitler tek kör grup tarafından makroskopik ve mikroskopik olarak değerlendirildi. Boyaların performansları Likert skalası kullanılarak en kötü (1) en iyi (5) olmak üzere değerlendirildi. Vakum uygulamasının yetersiz doku dehidrasyonundan kaynaklanan problemleri ortadan kaldırdığı görüldü. MMGG, at medulla spinalis’inde gri ve ak madde ayrımında en başarılı boya olarak tespit edildi.  \nAnahtarsözcükler: Gri madde oranı, At, Görüntüleme, Medulla spinalis, Boya karşılaştırması  \nINTRODUCTION  \nThe part of the central nervous system situated within the vertebral canal, namely, the spinal cord, is composed of 42-43 segments in horses, 8 of which are cervical, 18 thoracic, 6 lumbar, 5 sacral and 5-6 caudal. The spinal cord, enveloped by the","cbCaieFsQnFXeY7N","https://ap.wps.com/l/cbCaieFsQnFXeY7N","pdf",2315946,1,6,"English","en",105,"# Introduction\n## Study objective and rationale\n# Materials and Methods\n## Tissue sampling and processing\n## Staining methods and evaluation\n## Scoring approach\n# Results\n## Effect of vacuum dehydration\n## Best staining method selection\n# Discussion","[{\"question\":\"Why is gray and white matter staining important in spinal cord diagnosis?\",\"answer\":\"The ratio of gray and white matter is a significant clinical parameter for diagnosing diffuse and compressive spinal cord diseases.\"},{\"question\":\"How did vacuum application affect tissue processing in this study?\",\"answer\":\"Vacuum application reduced difficulties related to inadequate tissue dehydration during spinal cord dehydration.\"},{\"question\":\"Which staining method performed best for differentiating gray and white matter?\",\"answer\":\"Modified May-Grunwald-Giemsa (MMGG) was selected as the best method for distinguishing gray and white matter in the horse spinal cord.\"}]","Selective Gray and White Matter Staining of the Horse Spinal Cord - Research Article | PDF",1787692675,15,{"code":4,"msg":31,"data":32},"ok",{"site_id":24,"language":23,"slug":33,"title":13,"keywords":34,"description":14,"schema_data":35,"social_meta":87,"head_meta":89,"extra_data":91,"updated_unix":28},"selective-gray-and-white-matter-staining-of-the-horse-spinal-cord-research-article","",{"@graph":36,"@context":86},[37,54,69],{"@type":38,"itemListElement":39},"BreadcrumbList",[40,44,48,51],{"item":41,"name":42,"@type":43,"position":20},"https://docshare.wps.com","Home","ListItem",{"item":45,"name":46,"@type":43,"position":47},"https://docshare.wps.com/document/","Document",2,{"item":49,"name":12,"@type":43,"position":50},"https://docshare.wps.com/document/healthcare/",3,{"item":52,"name":13,"@type":43,"position":53},"https://docshare.wps.com/document/selective-gray-and-white-matter-staining-of-the-horse-spinal-cord-research-article/143079/",4,{"url":52,"name":13,"@type":55,"author":56,"headline":13,"publisher":58,"fileFormat":61,"inLanguage":23,"description":14,"dateModified":62,"datePublished":63,"encodingFormat":61,"isAccessibleForFree":64,"interactionStatistic":65},"DigitalDocument",{"name":9,"@type":57},"Person",{"url":41,"name":59,"@type":60},"DocShare","Organization","application/pdf","2026-08-29","2026-08-25",true,{"@type":66,"interactionType":67,"userInteractionCount":20},"InteractionCounter",{"@type":68},"ViewAction",{"@type":70,"mainEntity":71},"FAQPage",[72,78,82],{"name":73,"@type":74,"acceptedAnswer":75},"Why is gray and white matter staining important in spinal cord diagnosis?","Question",{"text":76,"@type":77},"The ratio of gray and white matter is a significant clinical parameter for diagnosing diffuse and compressive spinal cord diseases.","Answer",{"name":79,"@type":74,"acceptedAnswer":80},"How did vacuum application affect tissue processing in this study?",{"text":81,"@type":77},"Vacuum application reduced difficulties related to inadequate tissue dehydration during spinal cord dehydration.",{"name":83,"@type":74,"acceptedAnswer":84},"Which staining method performed best for differentiating gray and white matter?",{"text":85,"@type":77},"Modified May-Grunwald-Giemsa (MMGG) was selected as the best method for distinguishing gray and white matter in the horse spinal cord.","https://schema.org",{"og:url":52,"og:type":88,"og:title":13,"og:site_name":59,"og:description":14},"article",{"robots":90,"canonical":52},"index,follow",{"doc_id":7,"site_id":24},{"code":4,"msg":5,"data":93},[94,98,102,106,111,115,118,123,128,131,135],{"id":20,"doc_module":4,"doc_module_name":46,"category_name":95,"show_sort_weight":96,"slug":97},"Story & Novel",90,"story-novel",{"id":47,"doc_module":4,"doc_module_name":46,"category_name":99,"show_sort_weight":100,"slug":101},"Literature",80,"literature",{"id":53,"doc_module":4,"doc_module_name":46,"category_name":103,"show_sort_weight":104,"slug":105},"Exam",70,"exam",{"id":107,"doc_module":4,"doc_module_name":46,"category_name":108,"show_sort_weight":109,"slug":110},5,"Comic",60,"comic",{"id":21,"doc_module":4,"doc_module_name":46,"category_name":112,"show_sort_weight":113,"slug":114},"Technology",50,"technology",{"id":11,"doc_module":4,"doc_module_name":46,"category_name":12,"show_sort_weight":116,"slug":117},40,"healthcare",{"id":119,"doc_module":4,"doc_module_name":46,"category_name":120,"show_sort_weight":121,"slug":122},8,"Research & Report",30,"research-report",{"id":124,"doc_module":4,"doc_module_name":46,"category_name":125,"show_sort_weight":126,"slug":127},9,"Religion & Spirituality",20,"religion-spirituality",{"id":126,"doc_module":4,"doc_module_name":46,"category_name":129,"show_sort_weight":126,"slug":130},"World Cup","world-cup",{"id":132,"doc_module":4,"doc_module_name":46,"category_name":133,"show_sort_weight":132,"slug":134},10,"Lifestyle","lifestyle",{"id":136,"doc_module":4,"doc_module_name":46,"category_name":137,"show_sort_weight":107,"slug":138},19,"General","general"]