[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-331566-105":59,"doc-detail-331566-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","scrna-seq-analysis-revealed-cafs-regulating-hcc-cells-via-ptn-signaling","scRNA-Seq Analysis Revealed CAFs Regulating HCC Cells via PTN Signaling","","Cancer-associated fibroblasts (CAFs) are recognized as key regulators of the tumor microenvironment in hepatocellular carcinoma (HCC), yet the underlying mechanisms remain insufficiently clarified. Single-cell RNA sequencing datasets (GSE158723, GSE112271) were retrieved from GEO and analyzed with R software, supplemented by clinical sample experiments and in vitro cell assays. CAF proportions were higher in cancer tissues, with pleiotrophin (PTN) upregulated in tumors. SDC2 expression also increased in Huh7 cells versus LO2, supporting a CAF-driven PTN/SDC2 signaling contribution to HCC progression.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/scrna-seq-analysis-revealed-cafs-regulating-hcc-cells-via-ptn-signaling/331566/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/scrna-seq-analysis-revealed-cafs-regulating-hcc-cells-via-ptn-signaling/331566.png","ImageObject",300,407,{"name":92,"@type":93},"Seraphina","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-24","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What was the study's main focus regarding CAFs in HCC?","Question",{"text":112,"@type":113},"The study examined how cancer-associated fibroblasts (CAFs) may regulate hepatocellular carcinoma (HCC) cell development through pleiotrophin (PTN) and syndecan-2 (SDC2).","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were single-cell RNA sequencing datasets used in the analysis?",{"text":117,"@type":113},"Single-cell RNA sequencing datasets from GEO (GSE158723 and GSE112271) were retrieved and analyzed using R software to infer CAF-related interactions in the tumor microenvironment.",{"name":119,"@type":110,"acceptedAnswer":120},"What experimental findings supported the PTN/SDC2 signaling hypothesis?",{"text":121,"@type":113},"CAF proportions were higher in cancer tissues than adjacent non-cancerous tissues, PTN expression was elevated in cancer, and SDC2 was significantly upregulated in Huh7 cells compared with LO2 cells.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},331566,1790231831,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},962075114101,"https://ap-avatar.wpscdn.com/avatar/e000253a75eb197efd?x-image-process=image/resize,m_fixed,w_180,h_180&k=1780044092746381165","Journal of Hepatocellular Carcinoma Dovepress  \n Open Access Full Text Article ORIGINAL RESEARCH  \nscRNA-Seq Analysis Revealed CAFs Regulating HCC Cells via PTN Signaling  \nWenxian Lin 1–4 , Lizhu Tang 1 , 2 , 5 , Chenyi Zhuo 1 , Xiuli Mao 1–4 , Jiajia Shen 6 , Shaoang Huang 3 , Shangyang Li 3 , 7 , Yujuan Qin 3 ,4 , Ju Liao2 , 3 , Yuhong Chen 8 , Xiamin Zhang 3 ,4 , Yuting Li 9 , Jian Song4 , Lingzhang Meng 4 , Xiaofeng Dong 10 , Yueyong Li2  \n1Key Laboratory of Molecular Pathology for Hepatobiliary Diseases of Guangxi, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, The People’s Republic of China; 2Department of Interventional Oncology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, The People’s Republic of China; 3Graduate School, Youjiang Medical University for Nationalities, Baise, 533000, The People’s Republic of China; 4Institute of Cardiovascular Sciences, Guangxi Academy of Medical Sciences & The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530016, The People’s Republic of China; 5Department of Radiation Oncology, The First Affiliated Hospital of Jinan University, Guangzhou, 510632, The People’s Republic of China; 6Department of Laboratory Medicine, Nanning Maternity and Child Health Hospital & Nanning Women and Children’s Hospital, Nanning, 530011, The People’s Republic of China; 7Department of Laboratory Medicine, Guangxi Academy of Medical Sciences & The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530016, The People’s Republic of China; 8Department of Nephrology, the Second Affiliated Hospital of Guangxi Medical University, Nanning, 530007, The People’s Republic of China; 9Graduate School, Guangxi University of Chinese Medicine, Nanning, 530200, The People’s Republic of China; 10Department of Hepatobiliary, Pancreas and Spleen Surgery, Guangxi Academy of Medical Sciences & The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530016, The People’s Republic of China  \nCorrespondence: Yueyong Li; Xiaofeng Dong, Email [305017674@qq.com](305017674@qq.com); [gandanyingcai@163.com](gandanyingcai@163.com)  \n\n| Background: Cancer-associated fibroblasts (CAFs) play a pivotal role in shaping the microenvironment of hepatocellular carcinoma (HCC) . However, the mechanisms through which CAFs influence the progression of HCC remain incompletely understood. Methods: Single-cell RNA sequencing datasets (GSE158723 and GSE112271) were retrieved from the Gene Expression Omnibus (GEO) database at the National Center for Biotechnology Information (NCBI) and analyzed using R software. Our analysis suggested that CAFs may promote liver cancer cell development, possibly through the interaction of pleiotrophin (PTN) and syndecan-2 (SDC2) . Clinical samples from HCC patients were collected and processed into frozen sections and single-cell suspensions for Masson staining, immunofluorescence staining, and flow cytometry. Additionally, Huh7 liver cancer cells and LO2 normal liver cells were cultured and subjected to immunofluorescence assays using cell slides.\u003Cbr>Results: The proportion of CAFs in cancerous tissues was higher than in adjacent non-cancerous tissues, and pleiotrophin (PTN) expression was elevated in cancer tissues compared to adjacent tissues. These findings aligned with the results of the single-cell RNA sequencing (scRNA-seq) analysis. Furthermore, SDC2 expression was significantly upregulated in Huh7 liver cancer cells compared to LO2 normal liver cells.\u003Cbr>Discussion: This study suggests that CAFs may contribute to HCC progression via the PTN/SDC2 signaling pathway. Our findings provide deeper insights into the interactions between CAFs and HCC cells within the tumor microenvironment (TME) .\u003Cbr>Keywords: CAFs, HCC, PTN, scRNA-seq, TME |\n| --- |\n| Introduction\u003Cbr>Liver cancer poses a significant challenge. Since 2018, 841,000 new liver cancer cases have been reported worldwide, making it the fourth ","cbCaidBoyO2KsfkC","https://ap.wps.com/l/cbCaidBoyO2KsfkC","pdf",23845264,13,"English","# Background\n## Methods\n## Results\n## Discussion\n# Introduction","[{\"question\":\"What was the study's main focus regarding CAFs in HCC?\",\"answer\":\"The study examined how cancer-associated fibroblasts (CAFs) may regulate hepatocellular carcinoma (HCC) cell development through pleiotrophin (PTN) and syndecan-2 (SDC2).\"},{\"question\":\"How were single-cell RNA sequencing datasets used in the analysis?\",\"answer\":\"Single-cell RNA sequencing datasets from GEO (GSE158723 and GSE112271) were retrieved and analyzed using R software to infer CAF-related interactions in the tumor microenvironment.\"},{\"question\":\"What experimental findings supported the PTN/SDC2 signaling hypothesis?\",\"answer\":\"CAF proportions were higher in cancer tissues than adjacent non-cancerous tissues, PTN expression was elevated in cancer, and SDC2 was significantly upregulated in Huh7 cells compared with LO2 cells.\"}]","scRNA-Seq Analysis Revealed CAFs Regulating HCC Cells via PTN Signaling | PDF",1789994012,33]