[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-377186-105":59,"doc-detail-377186-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","relationships-between-physical-and-immunological-tumor-microenvironment-in-pancreatic-ductal-adenocarcinoma","Relationships between physical and immunological tumor microenvironment in pancreatic ductal adenocarcinoma","","Pancreatic ductal adenocarcinoma (PDAC) presents as a physically hard tumor and carries an unfavorable prognosis. A cohort of 93 patients undergoing radical surgery for pancreatic and bile duct cancers over 28 months was used to compare PDAC tissue stiffness with other neoplasms and to link tumor stiffness to pathological features. Physical and pathological analyses included patients with and without preoperative therapy, and the immunological tumor microenvironment was assessed via multiplex fluorescent immunohistochemistry. Tumor stiffness correlated with specific staining and stromal markers, and immune cell densities showed associations under non-therapy conditions.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/relationships-between-physical-and-immunological-tumor-microenvironment-in-pancreatic-ductal-adenocarcinoma/377186/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/relationships-between-physical-and-immunological-tumor-microenvironment-in-pancreatic-ductal-adenocarcinoma/377186.png","ImageObject",300,407,{"name":92,"@type":93},"Terk","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-27","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What was the study designed to determine about PDAC tissues?","Question",{"text":112,"@type":113},"The study estimated PDAC tissue stiffness relative to other neoplasms and assessed how tumor stiffness relates to pathological features.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was the immunological tumor microenvironment evaluated?",{"text":117,"@type":113},"It was evaluated using multiplexed fluorescent immunohistochemistry to quantify immune cell and tissue-region features.",{"name":119,"@type":110,"acceptedAnswer":120},"What differences were observed when comparing patients with and without preoperative therapy?",{"text":121,"@type":113},"Tumor stiffness was associated with immune cell infiltration only in cases without preoperative therapy, while the association was not shown after preoperative therapy.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},377186,1790298876,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":52,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144},1099525198933,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Received: 3 February 2023 | Revised: 26 April 2023 | Accepted: 5 May 2023  \nDOI: 10. 1111/cas.15853  \nOR I G I NAL ART I C L E  \nRelationships between physical and immunological tumor microenvironment in pancreatic ductal adenocarcinoma  \nYu Igata1,2  | Motohiro Kojima3  | Toshiyuki Suzuki4 | Genichiro Ishii5  | Ryo Morisue1,3 | Toshihiro Suzuki6,7 | Masashi Kudo1  | Motokazu Sugimoto1 |  \nShin Kobayashi1  | John D. Martin8 | Triantafyllos Stylianopoulos9 | Horacio Cabral10 | Mitsunobu R. Kano11 | Masaru Konishi1 | Naoto Gotohda1,2  \n1Department of Hepatobiliary and Pancreatic Surgery, National Cancer Center Hospital East, Kashiwa, Japan  \n2Course of Advanced Clinical Research of Cancer, Juntendo University Graduate School of Medicine, Tokyo, Japan 3 Division of Pathology, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Japan 4Department of Surgery, Hanyu General Hospital, Hanyu, Japan  \n5Department of Pathology and Clinical Laboratories, National Cancer Center Hospital East, Kashiwa, Japan 6Division of Pharmacology, School of Medicine, Teikyo University, Tokyo, Japan  \n7Department of Cancer Immunotherapy, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Japan 8 Red Arrow Therapeutics Co. , Ltd, Tokyo, Japan  \n9Cancer Biophysics Laboratory, Department of Mechanical and Manufacturing Engineering, University of Cyprus, Nicosia, Cyprus 10 Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan  \n11Department of Pharmaceutical Biomedicine, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama, Japan  \nCorrespondence  \nMotohiro Kojima, Division of Pathology, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, 6-5-1 Kashiwanoha, Kashiwa, Chiba 277-8577, Japan. [Email:](Email: mokojima@east. ncc.go.jp)[ mokojima@east. ncc.go.jp](Email: mokojima@east. ncc.go.jp)  \nFunding information  \nFund for the Promotion of Joint International Research, Grant/Award Number: 21K  \nAbstract  \nPancreatic ductal adenocarcinoma (PDAC) is physically palpated as a hard tumor with an unfavorable prognosis. Assessing physical features and their association with pathological features could help to elucidate the mechanism of physical abnormalities in cancer tissues. A total of 93 patients who underwent radical surgery for pancreatic and bile duct cancers at a single center hospital during a 28-month period were recruited for this study that aimed to estimate the stiffness of PDAC tissues compared to the other neoplasms and assess relationships between tumor stiffness and pathological features. Physical alterations and pathological features of PDAC, with or without preoperative therapy, were analyzed. The immunological tumor microenvironment was evaluated using multiplexed fluorescent immunohistochemistry. The stiffness of PDAC correlated with the ratio of Azan-Mallory staining, α-smooth muscle actin, and collagen I-positive areas of the tumors. Densities of CD8+ T cells and CD204+ macrophages were associated with tumor stiffness in cases without preoperative therapy. Pancreatic ductal adenocarcinoma treated with preoperative therapy  \nAbbreviations: αSMA, α-smooth muscle actin; ART, area of residual tumor; BDC, bile duct cancer; FFPE, formalin-fixed, paraffin-embedded; HABP, hyaluronan-binding protein; IPMN, intraductal papillary mucinous neoplasm; PDAC, pancreatic ductal adenocarcinoma; S-1, tegafur/gimeracil/oteracil; TME, tumor microenvironment.  \nThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.  \n© 2023 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.  \nwas softer than that withou","cbCaifKFHCWeTtQf","https://ap.wps.com/l/cbCaifKFHCWeTtQf","pdf",4293891,"English","# Abstract\n# Introduction","[{\"question\":\"What was the study designed to determine about PDAC tissues?\",\"answer\":\"The study estimated PDAC tissue stiffness relative to other neoplasms and assessed how tumor stiffness relates to pathological features.\"},{\"question\":\"How was the immunological tumor microenvironment evaluated?\",\"answer\":\"It was evaluated using multiplexed fluorescent immunohistochemistry to quantify immune cell and tissue-region features.\"},{\"question\":\"What differences were observed when comparing patients with and without preoperative therapy?\",\"answer\":\"Tumor stiffness was associated with immune cell infiltration only in cases without preoperative therapy, while the association was not shown after preoperative therapy.\"}]","Relationships between physical and immunological tumor microenvironment in pancreatic ductal adenocarcinoma | PDF",1790223620,25]