[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-329044-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-329044-en":131},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","reconstructing-oral-cavity-tumor-evolution-through-brush-biopsy","Reconstructing oral cavity tumor evolution through brush biopsy","","Oral potentially malignant disorders (OPMDs) carry a heightened risk of progressing to oral squamous cell carcinoma (OSCC), yet current genomic assessment relies on invasive tissue biopsy. This pilot study evaluates whether brush biopsy can accurately reconstruct the genomic profile and tumor evolution. Paired tissue and brush samples were analyzed for SNVs, CNA profiles, and subclonal architectures, showing high SNV capture and similar CNA patterns across matched lesions, with shared ancestry across OPMD and OSCC.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/reconstructing-oral-cavity-tumor-evolution-through-brush-biopsy/329044/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/reconstructing-oral-cavity-tumor-evolution-through-brush-biopsy/329044.png","ImageObject",300,407,{"name":42,"@type":43},"Riley","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-27","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",11,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What problem does the study address regarding OPMD and OSCC genomics?","Question",{"text":63,"@type":64},"Genomic data are usually obtained through invasive tissue biopsy, while it remains uncertain whether non-invasive brush biopsy reflects the genomic landscape of OPMD and tumor evolution to OSCC.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"Which genomic features were compared between tissue and brush biopsy samples?",{"text":68,"@type":64},"The study analyzed single nucleotide variants (SNVs), copy number aberrations (CNAs), and subclonal architectures in paired tissue and brush samples.",{"name":70,"@type":61,"acceptedAnswer":71},"What did the results show about the performance of brush biopsy?",{"text":72,"@type":64},"Brush biopsy captured 90% of SNVs and produced CNA profiles similar to those seen in paired tissue biopsies across all lesions, while normal buccal mucosa did not share these alterations.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},329044,1790091349,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,111,115,120,123,127],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":108,"show_sort_weight":109,"slug":110},7,"Healthcare",40,"healthcare",{"id":112,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":113,"slug":114},8,30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":124,"doc_module":4,"doc_module_name":25,"category_name":125,"show_sort_weight":124,"slug":126},10,"Lifestyle","lifestyle",{"id":128,"doc_module":4,"doc_module_name":25,"category_name":129,"show_sort_weight":97,"slug":130},19,"General","general",{"code":4,"msg":82,"data":132},{"doc_id":79,"user_id":133,"nickname":42,"user_avatar":134,"doc_module":4,"category_id":112,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":116,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145,"preview_page_count":4},1374391975076,"https://ap-avatar.wpscdn.com/avatar/14000253ca4ec9f6853?x-image-process=image/resize,m_fixed,w_180,h_180&k=1783305029341752051","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nReconstructing oral cavity tumor evolution through brush biopsy  \nEvit John1, Tom Lesluyes2, Toby M. Baker1,2, MaximeTarabichi3, Ann Gillenwater4, Jennifer R. Wang4, Peter Van Loo1,2,5,6 & Xiao Zhao1,4,6􀀍  \nOral potentially malignant disorders (OPMDs) with genomic alterations have a heightened risk of evolving into oral squamous cell carcinoma (OSCC). Currently, genomic data are typically obtained through invasive tissue biopsy. However, brush biopsy is a non-invasive method that has been utilized for identifying dysplastic cells in OPMD but its effectiveness in reflecting the genomic landscape of OPMDs remains uncertain. This pilot study investigates the potential of brush biopsy samples inaccurately reconstructing the genomic profile and tumor evolution in a patient with both OPMD and OSCC. We analyzed single nucleotide variants (SNVs), copy number aberrations (CNAs), and subclonal architectures in paired tissue and brush biopsy samples. The results showed that brush biopsy effectively captured 90% ofSNVs and had similar CNA profiles as those seen in its paired tissue biopsies in all lesions. It was specific, as normal buccal mucosa did not share these genomic alterations. Interestingly, brush biopsy revealed shared SNVs and CNAs between the distinct OPMD andOSCC lesions from the same patient, indicating a common ancestral origin. Subclonal reconstruction confirmed this shared ancestry, followed by divergent evolution of the lesions. These findings highlight the potential of brush biopsies in accurately representing the genomic profile of OPL and OSCC, proving insight into reconstructing tumor evolution.  \nOral cancer is a significant public health concern worldwide with more than 90% of the oral cavity malignancies being oral squamous cell carcinomas (OSCC)1. Some of these lesions develop from oral potentially malignant disorders (OPMDs) . OPMDs are a group of conditions that possess the risk of malignant transformation to OSCC2,3. Risk of transformation varies based on the type of lesion and pathologic severity, with an overall transformation risk across all OPMDs being 7.9%4. Once transformed, patients with OSCC have an overall 5-year survival rate of 64.3% .  \nIt is known that genomic alterations increase the risk of transformation from OPMD to OSCC5,6. Copy number alterations (CNAs) in specific regions of interest such as 3p, 9p, 17p loss as well as loss of heterozygosity (LOH) in genes associated with OSCC such as TP53, CASP8, and CDKN2A are predictive of malignant transformation of OPMD7–11.  \nHowever, the tumor evolution and the clonal selection of these genomic events are not as well understood as in other cancers. For example, in colorectal cancer, the transition from adenoma to carcinoma is characterized by the early accumulation of specific genetic alterations such as mutation in APC, KRAS, and TP53 genes12. Similarly, in breast cancer, the progression from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC) involves a series of genetic and epigenetic changes, including clonal alterations in TP53, BRCA1, and PIK3CA13.  \nTo study genomic alterations and tumor evolution, the gold standard is longitudinal tissue sampling. Obtaining tissue to study genomic alterations sometimes coincides with treatment for OPMDs, which can involve surgical biopsy and surgical resection and/or laser ablation. However, longitudinal serial biopsies and biopsies for only research purposes result in morbidity and is rarely acceptable for patients. As such, a noninvasive technique of obtaining tissue would be ideal to study tumor evolution. Cytological study of oral cells is a non-invasive technique that is well accepted by the patient for the diagnosis of dysplastic cells in OPMDs and could be an attractive option to study tumor evolution in OPMD14, 15. Indeed, there is evidence that brush biopsy is able to capture genomic alterations in target ","cbCaicNYIMAXVQvU","https://ap.wps.com/l/cbCaicNYIMAXVQvU","pdf",2191934,"English","# Background\n## Public health burden and transformation risk\n## Genomic predictors of malignant progression\n# Need for non-invasive sampling\n## Limitations of longitudinal tissue biopsy\n## Brush biopsy as an alternative\n# Study design and analytical approach\n## Sample pairing and genomic analyses\n# Key findings\n## Concordance of SNVs and CNAs\n## Shared ancestry and divergent evolution\n# Implications","[{\"question\":\"What problem does the study address regarding OPMD and OSCC genomics?\",\"answer\":\"Genomic data are usually obtained through invasive tissue biopsy, while it remains uncertain whether non-invasive brush biopsy reflects the genomic landscape of OPMD and tumor evolution to OSCC.\"},{\"question\":\"Which genomic features were compared between tissue and brush biopsy samples?\",\"answer\":\"The study analyzed single nucleotide variants (SNVs), copy number aberrations (CNAs), and subclonal architectures in paired tissue and brush samples.\"},{\"question\":\"What did the results show about the performance of brush biopsy?\",\"answer\":\"Brush biopsy captured 90% of SNVs and produced CNA profiles similar to those seen in paired tissue biopsies across all lesions, while normal buccal mucosa did not share these alterations.\"}]","Reconstructing oral cavity tumor evolution through brush biopsy | PDF",1789982219,23]