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This study investigated the function and molecular mechanism of the ribosome biogenesis factor RBIS in non-small cell lung cancer progression. Methods The work integrated Gene Set Enrichment Analysis and Gene Expression Omnibus data to identify ribosome-associated differentially expressed genes, followed by in vitro assays, xenograft models, flow cytometry, qRT-PCR, polysome profiling, global protein synthesis measurement, immunofluorescence, and Co-IP/Western blot. Results RBIS was upregulated in lung adenocarcinoma and linked to poor prognosis; RBIS knockdown suppressed migration, invasion, proliferation, tumor growth, and metastasis, induced G0/G1 arrest and apoptosis, impaired ribosome RNA expression and translation efficiency, and enhanced synergy with CX-5461 and gemcitabine, with GNL2 identified downstream. Conclusions RBIS plays a close role in lung adenocarcinoma progression, supporting ribosome-targeted therapeutic strategies.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/rbis-regulates-ribosome-biogenesis-to-affect-progression-in-lung-adenocarcinoma/329703/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/rbis-regulates-ribosome-biogenesis-to-affect-progression-in-lung-adenocarcinoma/329703.png","ImageObject",300,407,{"name":92,"@type":93},"Genevieve","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main biological focus of this study?","Question",{"text":112,"@type":113},"The study examines how the ribosome biogenesis factor RBIS regulates ribosome biogenesis and influences progression in lung adenocarcinoma.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were RBIS-associated genes identified?",{"text":117,"@type":113},"RBIS-linked ribosome-associated genes were identified by intersecting gene sets from GSEA and differential expression results from GEO datasets.",{"name":119,"@type":110,"acceptedAnswer":120},"What effects did RBIS knockdown produce in lung cancer models?",{"text":121,"@type":113},"RBIS knockdown inhibited migration, invasion, and proliferation in vitro and suppressed tumor growth and metastasis in vivo, accompanied by G0/G1 arrest and increased apoptosis.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},329703,1790176249,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},1374391974585,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","Pan etal. Journal of Translational Medicine (2024) 22:1147  \n[https://doi.org/10.1186/s12967-024-05886-1](https://doi.org/10.1186/s12967-024-05886-1)  \nJournal of Translational Medicine  \n RESEARCH Open Access  \nRBIS regulates ribosome biogenesis to affect   progression in lung adenocarcinoma  \nHongyu Pan1,2†, Li Liao3†, Siwei Xu1,2, Yujian Xu1,2, Wenjun Chai1,2, Xiaoli Liu1,2, Jing Li 1,2, Yue Cao1,2, Lei Sun1,2, Qian Liu4* and Mingxia Yan1,2*  \nAbstract  \nBackground Increased ribosome biogenesis is required for tumor growth. In this study, we investigated the function and underlying molecular mechanism of ribosome biogenesis factor (RBIS) in the progression of non-small cell lung cancer (NSCLC) .  \nMethods In our study, we conducted a comprehensive analysis to identify key genes implicated in ribosome biogenesis by leveraging a Gene Set Enrichment Analysis (GSEA) dataset. Subsequently, we performed a comparative analysis of gene expression profiles by utilizing data from the Gene Expression Omnibus (GEO) datasets to ascertain differentially expressed genes (DEGs) between cancerous and adjacent non-cancerous tissues. Through the intersection of gene sets derived from GSEA and GEO, we identified a cohort of ribosome-associated genes that might exert a substantial influence on the progression of lung adenocarcinoma. Following an extensive literature review, we have identified the RBIS gene as an interesting candidate for further investigation. To elucidate the in vitro functional role of RBIS, several assays was employed, including the Transwell migration and invasion assay, wound healing assay, Cell Counting Kit-8 (CCK-8) proliferation assay, and colony formation assay. Subcutaneous and tail vein injection-based lung metastasis xenograft tumor models were used in evaluating the tumorigenic potential, growth, and metastatic spread of lung cancer cells. Flow cytometry analysis was employed to investigate cell cycle distribution and apoptotic rates. Additionally, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was utilized to quantify the mRNA expression levels of genes. To comprehensively assess the translational efficiency of nascent proteins, we employed polysome profiling analysis to provide insights into the cellular translational landscape. Furthermore, we quantified global protein synthesis using a fluorescence-based assay to measure protein synthesis rates. The immunofluorescence technology was utilized to study the subcellular reorganization of the nucleolus. We conducted co-immunoprecipitation (Co-IP) assays followed by Western blot analysis to identify potential proteins interacted with RBIS. The half maximal inhibitory concentration (IC50) was used for evaluating the chemosensitivity of lung cancer cells to gemcitabine. Additionally, the colony formation assay was employed to assess the survival and proliferative capacity post-treatment of gemcitabine.  \nResults The database analysis showed that RBIS was upregulated in lung adenocarcinoma, and its high expression was associated with poor prognosis; Knockdown of RBIS significantly inhibited NSCLC cell migration, invasion and proliferation in vitro and xenograft tumor growth and metastasis in vivo. Additionally, knockdown of RBIS led  \n†Hongyu Pan and Li Liao contributed equally to this work.  \n*Correspondence:  \nQian Liu [liuqian01@xinhuamed.com.cn](liuqian01@xinhuamed.com.cn)[ ](liuqian01@xinhuamed.com.cn)Mingxia Yan [mingxiayan@shca.org.cn](mingxiayan@shca.org.cn)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you mo","cbCaim9votwbkuZL","https://ap.wps.com/l/cbCaim9votwbkuZL","pdf",6815295,17,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions","[{\"question\":\"What is the main biological focus of this study?\",\"answer\":\"The study examines how the ribosome biogenesis factor RBIS regulates ribosome biogenesis and influences progression in lung adenocarcinoma.\"},{\"question\":\"How were RBIS-associated genes identified?\",\"answer\":\"RBIS-linked ribosome-associated genes were identified by intersecting gene sets from GSEA and differential expression results from GEO datasets.\"},{\"question\":\"What effects did RBIS knockdown produce in lung cancer models?\",\"answer\":\"RBIS knockdown inhibited migration, invasion, and proliferation in vitro and suppressed tumor growth and metastasis in vivo, accompanied by G0/G1 arrest and increased apoptosis.\"}]","RBIS regulates ribosome biogenesis to affect progression in lung adenocarcinoma | PDF",1789985246,43]