[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-450211-105":59,"doc-detail-450211-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","rapid-enantioselective-fluorescence-recognition-and-chiral-separation-of-free-amino-acids","Rapid enantioselective fluorescence recognition and chiral separation of free amino acids","","Rapid enantioselective recognition and practical chiral separation of free amino acids are achieved using a water-soluble chiral fluorescent probe. The probe is built by incorporating a morpholinium quaternary cation into 1,1’-bi-2-naphthol frameworks, enabling visual discrimination of amino-acid enantiomers within 100 s via luminescence color/intensity changes. The mechanism involves imine formation, electrostatic interactions, and aggregation-induced emission, leading to selective aggregation and precipitation. Enantiomers are separated from D-/L mixtures by simple filtration, validated by fluorescence visualization and chiral HPLC.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/rapid-enantioselective-fluorescence-recognition-and-chiral-separation-of-free-amino-acids/450211/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/rapid-enantioselective-fluorescence-recognition-and-chiral-separation-of-free-amino-acids/450211.png","ImageObject",300,407,{"name":92,"@type":93},"Ezra","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-08","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":24},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"How does the fluorescent probe achieve rapid chiral discrimination of free amino acids?","Question",{"text":112,"@type":113},"It incorporates a morpholinium quaternary cation into a 1,1’-bi-2-naphthol framework, producing distinct luminescence color or intensity changes upon binding enantiomers within 100 s.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What mechanism drives the enantioselective recognition and separation?",{"text":117,"@type":113},"The study attributes the behavior to imine formation and electrostatic interactions together with aggregation-induced emission, which promotes selective aggregation and precipitation with specific enantiomers.",{"name":119,"@type":110,"acceptedAnswer":120},"How are the amino acid enantiomers separated from D-/L mixtures?",{"text":121,"@type":113},"The enantiomers can be separated through a simple filtration process after the probe selectively precipitates with specific enantiomers.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},450211,1790819140,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":24,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},1099514068035,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","Article [https://doi.org/10.1038/s41467-025-68144-y](https://doi.org/10.1038/s41467-025-68144-y)  \nRapid enantioselective fluorescence recognition and chiral separation of free amino acids  \nReceived: 14 September 2025  \n\n| Accepted: 19 December 2025 |\n| --- |\n| |\n| Check for updates |\n\nYang Li 1,3, Kang Yu1,3, Zhiyong Xu1,3, Jie Zeng1, Jinyu Wei1, Haipeng Jiang1, Yuanyuan Zhu 1 , Shuangxi Gu 1  & Xiang Ma 2   \nEnantioselective recognition and chiral separation of amino acids hold significant importance in chemistry, materials science, and life science. Here, wereport a water-soluble chiral fluorescent probe that enables visual chiral recognition and separation by incorporating a morpholinium quaternary cation into the 1,1’-bi-2-naphthol frameworks. Upon binding with free amino acid enantiomers, the probe achieves rapid chiral discrimination within 100 s, accompanied by distinct changes in luminescence color or intensity. The underlying mechanism of this chiral recognition involves imine formation and electrostatic interactions, accompanied by aggregation-induced emission. These processes collectively promote selective aggregation and precipitation between the probe and specific enantiomers of amino acids. Furthermore, the enantiomers can be efficiently separated from D-/L-amino acid mixtures through a simple filtration process. Comparative analyses using a fluorescence visualization and chiral high performance liquid chromatography further validate the probe’sefficacy in achieving efficient chiral separation. This study provides a practical approach for the precise detection and separation of amino acid enantiomers.  \nOptically pure amino acids play a critical role in elucidating the fundamental principles of life, developing safe and effective pharmaceuticals, facilitating chemical synthesis, and advancing applications in fields such as food nutrition and industrial biotechnology1–5. Therefore, the enantioselective recognition and separation of amino acids are of great importance6–8. A variety of analytical and detection techniques have been established for determining the enantiomeric composition of chiral amino acids, including nuclear magnetic resonance (NMR), ultraviolet/visible (UV/vis) spectroscopy, circular dichroism (CD) spectroscopy, high-performance liquid chromatography (HPLC), and fluorescence/phosphorescence methods9–13. Among them,  \nenantiomer recognition based on fluorescence intensity and wavelength has garnered increasing attention owing to its high sensitivity, cost-effectiveness, and ease of operation14–20. To date, various fluorescent probes based on chiral molecules, supramolecular assemblies, and inorganic nanomaterials have been developed for the detection of amino acid enantiomers21–23. However, most of these probes exhibit relatively long response times (typically exceeding 30 min) towardspecific enantiomers, which hinders their broader applications24–27. Furthermore, although these probes demonstrate effectiveness in fluorescence-based chiral recognition, few possess the capability to enable practical chiral separation of amino acid enantiomers.  \n1Hubei Key Laboratory of Novel Reactor and Green Chemical Technology, Key Laboratory for Green Chemical Process of Ministry of Education, State Key Laboratory of Green and Efficient Development of Phosphorus Resources, Pharmaceutical Research Institute, School of Chemical Engineering and Pharmacy, School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan, China. 2Key Laboratory for Advanced Materials and Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China. 3These authors contributed equally: Yang Li, Kang Yu, Zhiyong Xu.  \ne-mail: [yyzhu531@163.com](yyzhu531@163.com); [shuangxigu@163.com](shuangxigu@163.com); [maxiang@ecust.edu.cn](maxiang@ecust.edu.cn)  \nEnantiome","cbCaig52JcSnXJAd","https://ap.wps.com/l/cbCaig52JcSnXJAd","pdf",3196872,11,"English","# Introduction\n## Fluorescence-based enantiomer recognition\n## Limitations of existing fluorescent probes\n# Materials and Methods (as described)\n## Design of the chiral fluorescent probe\n## Detection and separation approach\n# Results and Validation (as described)\n## Fluorescence visualization and chiral HPLC validation","[{\"question\":\"How does the fluorescent probe achieve rapid chiral discrimination of free amino acids?\",\"answer\":\"It incorporates a morpholinium quaternary cation into a 1,1’-bi-2-naphthol framework, producing distinct luminescence color or intensity changes upon binding enantiomers within 100 s.\"},{\"question\":\"What mechanism drives the enantioselective recognition and separation?\",\"answer\":\"The study attributes the behavior to imine formation and electrostatic interactions together with aggregation-induced emission, which promotes selective aggregation and precipitation with specific enantiomers.\"},{\"question\":\"How are the amino acid enantiomers separated from D-/L mixtures?\",\"answer\":\"The enantiomers can be separated through a simple filtration process after the probe selectively precipitates with specific enantiomers.\"}]","Rapid enantioselective fluorescence recognition and chiral separation of free amino acids | PDF",1790732471,28]