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The study tested whether QUE mitigates lipopolysaccharide (LPS)-induced PANoptosis in broiler bursal cells through the cGAS/STING signaling pathway. In vivo treatments included Control, QUE (10 mg/kg), LPS (0.15 mg/kg), and LPS+QUE; in vitro DT40 cells received LPS (40 μg/mL) and QUE (10 μM), with MSA-2 to confirm pathway involvement. QUE reduced oxidative stress, corrected mitochondrial dynamics, limited mitochondrial metabolic disruption, decreased cytoplasmic mtDNA, suppressed cGAS/STING activation, and attenuated PANoptosis and inflammatory responses.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/quercetin-mitigated-lps-induced-panoptosis-in-the-bursa-of-fabricius-via-the-cgassting-signaling-pathway-in-broilers-article/415637/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/quercetin-mitigated-lps-induced-panoptosis-in-the-bursa-of-fabricius-via-the-cgassting-signaling-pathway-in-broilers-article/415637.png","ImageObject",300,407,{"name":92,"@type":93},"Sarah ","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-28",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the primary objective of this study?","Question",{"text":112,"@type":113},"To determine whether quercetin can mitigate LPS-induced PANoptosis in broiler bursal cells via the cGAS/STING signaling pathway.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were the experiments designed in vivo and in vitro?",{"text":117,"@type":113},"White Leghorn chickens were assigned to Control, QUE, LPS, and LPS+QUE groups; DT40 cells were treated with LPS, QUE, or both, and MSA-2 was used to verify pathway involvement.",{"name":119,"@type":110,"acceptedAnswer":120},"What mechanisms did QUE influence according to the results?",{"text":121,"@type":113},"QUE reduced oxidative stress, corrected mitochondrial dynamics imbalance, limited mitochondrial metabolic dysfunction, decreased cytoplasmic mtDNA, suppressed cGAS/STING activation, and ultimately attenuated PANoptosis and inflammatory responses.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},415637,1790716773,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},962085320529,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","105 (2026) 106179  \nContents lists available at ScienceDirect  \nPoultry Science  \njournal [homepage:](homepage: www.elsevier.com/locate/psj)[ www.elsevier.com/locate/psj](homepage: www.elsevier.com/locate/psj)  \n| Quercetin mitigated LPS-induced PANoptosis in the bursa of Fabricius via the cGAS/STING signaling pathway in broilers\u003Cbr>Yiming Zhang a,b, Qihang Jiang a, Haodong Hua, Shiping Li c, Jiahong Chua, Fuze Shea, Shu Li a,d,* \u003Cbr>a College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China b College of Life Science, Northeast Agricultural University, Harbin 150030, China\u003Cbr>c College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China\u003Cbr>d Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China |  |  |\n| --- | --- | --- |\n| A R T I C L E I N F O |  | A B S T R A C T |\n| Keywords: Quercetin PANoptosis Bursa of fabricius Mitochondria\u003Cbr>cGAS/STING signaling pathway |  | Quercetin (QUE), a natural bioflavonoid with anti-inflammatory and antioxidant properties, regulates immune organ function by enhancing antioxidant capacity and maintaining mitochondrial homeostasis. This study aims to explore whether QUE can mitigate lipopolysaccharide (LPS)-induced PANoptosis in broilers bursal cells via cyclic GMP-AMP synthase (cGAS)/stimulator of interferon genes (STING) pathway. In vivo, White Leghorn chickens were assigned to Control, QUE (10 mg/kg gavage), LPS (0.15 mg/kg intraperitoneal injection), and LPS+QUE groups. In vitro, brolier lymphoma cells (DT40) were treated with LPS (40 μg/mL), QUE (10 μM), or their combination; STING agonist MSA-2 (2 μM) was used to verify pathway involvement. Hematoxylin-Eosin (H&E) staining, TUNEL assay, immunofluorescence, reactive oxygen species (ROS) detection, antioxidant index assays, quantitative real-time PCR (qRT-PCR), and Western blot (WB) were employed to detect factors related to PANoptosis, inflammation, mitochondrial dynamics, mitochondrial metabolism and the cGAS/STING signaling pathway. Results demonstrated that QUE effectively mitigated LPS-induced oxidative stress, mitochondrial dynamics imbalance, and mitochondrial metabolic dysfunction in broiler bursal cells and DT40 cells. Consequently, QUE reduced the release of mitochondrial DNA (mtDNA) into the cytoplasm, suppressed the activation of the cGAS/STING signaling pathway, and ultimately attenuated the occurrence of PANoptosis and inflammatory responses. This study confirms that QUE exerts a protective effect against LPS-induced PANoptosis via cGAS/STING pathway in the broiler bursa of Fabricius, thereby providing a novel theoretical basis for the application of QUE as a green feed additive. Notably, this finding underscores the potential of QUE as a natural and effective alternative to traditional antibiotics in animal feed, which is of great significance for reducing antibiotic dependence and facilitating the sustainable development of the poultry farming industry. |\n\nAbbreviations: AO/EB, Acridine orange/Ethidium bromide; ATP, Adenosine triphosphate; ATP5A1, Mitochondrial F1 complex; ATPase, Adenosine triphosphatase; AvBDs, Avian β-defensins; Caspase-3, Cysteinyl aspartate specific proteinase-3; Caspase-8, Cysteinyl aspartate specific proteinase-8; CAT, Catalase; C-Caspase-3, Cleaved-cysteinyl aspartate specific proteinase-3; cGAS, Cyclic GMP-AMP synthase; DHE, Dihydroethidium; DRP1, Dynamin-related protein 1; GSDMD, Gasdermin D; GSH, Glutathione; H&E, Hematoxylin&Eosin; IFN-γ, Interferon γ; Ig A, Immunoglobulin A; Ig G, Immunoglobulin G; Ig M, Immunoglobulin M; IL-1β, Interleukin 1β; IL-6, Interleukin 6; LPS, Lipopolysaccharide; MFN1, Mitofusin 1; MFN2, Mitofusin 2; MLKL, Mixed Lineage Kinase Domain-Like Protein; MTCO1, Mitochondrially Encoded Cytochrome c Oxidase I; NDUFB8, Ubiquinone oxidoreductase subunit B8; O","cbCaimmGfCEfNv2f","https://ap.wps.com/l/cbCaimmGfCEfNv2f","pdf",10382305,14,"English","# Introduction\n## Study rationale and background\n## Methods overview\n## Results overview\n## Conclusion and implications","[{\"question\":\"What is the primary objective of this study?\",\"answer\":\"To determine whether quercetin can mitigate LPS-induced PANoptosis in broiler bursal cells via the cGAS/STING signaling pathway.\"},{\"question\":\"How were the experiments designed in vivo and in vitro?\",\"answer\":\"White Leghorn chickens were assigned to Control, QUE, LPS, and LPS+QUE groups; DT40 cells were treated with LPS, QUE, or both, and MSA-2 was used to verify pathway involvement.\"},{\"question\":\"What mechanisms did QUE influence according to the results?\",\"answer\":\"QUE reduced oxidative stress, corrected mitochondrial dynamics imbalance, limited mitochondrial metabolic dysfunction, decreased cytoplasmic mtDNA, suppressed cGAS/STING activation, and ultimately attenuated PANoptosis and inflammatory responses.\"}]","Quercetin mitigated LPS-induced PANoptosis in the bursa of Fabricius via the cGAS/STING signaling pathway in broilers - Article | PDF",1790602258,35]