[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-329898-105":59,"doc-detail-329898-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","quantification-of-the-most-effective-x-ray-dosage-for-inhibiting-invasion-of-glioma-cell-line","Quantification of the Most Effective X-Ray Dosage for Inhibiting Invasion of Glioma Cell Line","","Experimental research quantifies how 6MV X-ray radiotherapy affects C6 glioblastoma cell behavior across absorbed doses from 4 to 10 Gy in 2 Gy steps. Cell responses were evaluated using toxicity readouts alongside migration and colony formation assays, with outcomes compared across the dose range. Results show dose-dependent cytotoxicity, accompanied by a significant reduction in migration ability and colony-forming capacity. Findings clarify how ionizing radiation disrupts malignant progression through direct cellular functional impairment.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/quantification-of-the-most-effective-x-ray-dosage-for-inhibiting-invasion-of-glioma-cell-line/329898/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/quantification-of-the-most-effective-x-ray-dosage-for-inhibiting-invasion-of-glioma-cell-line/329898.png","ImageObject",300,407,{"name":92,"@type":93},"Valentina","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What dose range of 6MV X-ray radiotherapy was tested on C6 glioblastoma cells?","Question",{"text":112,"@type":113},"Absorbed doses ranged from 4 to 10 Gy, administered in 2 Gy steps (4, 6, 8, and 10 Gy).","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How did X-ray radiotherapy affect C6 cell viability and toxicity?",{"text":117,"@type":113},"Cytotoxic responses increased in a dose-dependent manner, indicating higher toxicity at higher absorbed doses.",{"name":119,"@type":110,"acceptedAnswer":120},"Which assays were used to evaluate the effects of radiotherapy on C6 cells?",{"text":121,"@type":113},"The study used MTT assays to assess proliferation/viability, colony formation assays to evaluate clonogenic ability, and cell migration assays to measure migratory behavior.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},329898,1790195040,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":24,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},13056703020460,"https://ap-avatar.wpscdn.com/avatar/be000253dac470eee5d?_k=1778207105932848923","RESEARCH ARTICLE  \nEditorial Process: Submission:07/30/2024 Acceptance:12/15/2024  \nQuantification of the Most Effective X-Ray Dosage for Inhibiting Invasion of Glioma Cell Line  \nElham Ghasemi1, Zeynab Zamanzade1, Fatemeh Seif2, Mahdieh Mondanizadeh3* Abstract  \nBackground: One key feature that distinguishes cancerous cell populations from their normal counterparts is a heightened tendency towards uncontrolled growth and invasive activity. Therapeutic techniques like radiotherapy can impact the viability and invasive behavior of cancer cells by modifying the structure of DNA and inducing programmed cell death. Methods: This research is an experimental study and involved a comprehensive investigation into the effects of 6MV X-ray radiotherapy on various absorbed doses (ranging from 4 to 10 Gy by steps 2Gy) on toxicity, migration, and colony formation in C6 glioblastoma cellular cultures. Results: Our detailed analysis revealed that the cytotoxic responses increased in a dose-dependent manner, while there was a significant decrease in both the ability to migrate and form colonies in the C6 cell line. Conclusions: This thorough examination provides new insights into the way malignant cell populations respond to ionizing radiation in a dose-dependent manner within a clinical setting. By directly impacting cellular functions and causing disturbances, this occurrence leads to a slowing down of disease progression.  \nKeywords: Glioma-C6 cells-MTTAssay-X-ray radiotherapy  \nAsian Pac J Cancer Prev, 25 (12), 4299-4303  \nIntroduction  \nGlioma is a type of abnormal cell growth that develops from glial cells in the central nervous system, mainly in the brain and spinal cord [1, 2] . While gliomas can occur in people of different ages, they are more common in adults [3] . Symptoms of glioma include persistent headaches, changes in cognitive and neurological functions, seizures, and difficulties with motor skills [2, 4] . Analysis of population-based data from the United States reveals that gliomas constitute approximately 27% of the total incidence of brain tumors [5] .  \nThe therapeutic modalities available for glioma encompass surgical intervention, radiation therapy, chemotherapy, targeted therapy, immunotherapy, and supportive care [6, 7] . Among the available treatment modalities, radiation therapy has emerged as a prominent and esteemed therapeutic approach for glioma [8] . It is characterized by its remarkable safety profile and substantial therapeutic effectiveness. This approach utilizes ionizing radiation delivered through high-energy beams to elicit cytotoxic effects or impede the proliferation of malignant glioma cells [9]. The therapeutic application of X-ray radiation in glioma treatment involves the utilization of high-energy X-rays to selectively target and eradicate neoplastic cells within the brain [10]. radiotherapy denotes  \nthe precise energy level of the radiation beam employed in this modality. External beam radiation therapy, facilitated by a linear accelerator apparatus, is the customary method employed for administering this treatment [11] . The primary objective is to deliver accurate and controlled doses of radiation to the tumor, minimizing adverse effects on adjacent healthy tissues, and thereby facilitating tumor reduction and preventing recurrence [12]. X-ray radiation investigations have yielded valuable insights into the molecular and cellular mechanisms underlying DNA damage and repair, as well as the apoptotic pathways activated in response to varying doses of X-ray irradiation [13] . These studies have provided evidence of elevated levels of reactive oxygen species (ROS), DNA doublestrand breaks (DSB), cellular apoptosis, alterations in G2/M phase distribution, and suppression of cellular proliferation subsequent to irradiation [14, 15]. However, following radiation exposure, there is an increase in the expression of proteins associated with apoptosis, such as Bcl-2-associated X protein (Bax), caspase-9","cbCaiuI8apg4BMaM","https://ap.wps.com/l/cbCaiuI8apg4BMaM","pdf",512846,"English","# Introduction\n# Materials and Methods\n## Cell culture","[{\"question\":\"What dose range of 6MV X-ray radiotherapy was tested on C6 glioblastoma cells?\",\"answer\":\"Absorbed doses ranged from 4 to 10 Gy, administered in 2 Gy steps (4, 6, 8, and 10 Gy).\"},{\"question\":\"How did X-ray radiotherapy affect C6 cell viability and toxicity?\",\"answer\":\"Cytotoxic responses increased in a dose-dependent manner, indicating higher toxicity at higher absorbed doses.\"},{\"question\":\"Which assays were used to evaluate the effects of radiotherapy on C6 cells?\",\"answer\":\"The study used MTT assays to assess proliferation/viability, colony formation assays to evaluate clonogenic ability, and cell migration assays to measure migratory behavior.\"}]","Quantification of the Most Effective X-Ray Dosage for Inhibiting Invasion of Glioma Cell Line | PDF",1789986075,13]