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It outlines kit contents, shipping and storage requirements, intended use for molecular biology applications, and key safety information. The handbook includes quality control, the underlying principle and procedure, required equipment and reagents, important notes, detailed purification protocols for different kit formats, troubleshooting, and appendices for agarose gel analysis and buffer compositions.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/qiagen-plasmid-purification-handbook-plasmid-mini-midi-maxi-mega-and-giga-kits-for-purification-of-ultrapure-transfection-grade-plasmid-dna/402463/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/qiagen-plasmid-purification-handbook-plasmid-mini-midi-maxi-mega-and-giga-kits-for-purification-of-ultrapure-transfection-grade-plasmid-dna/402463.png","ImageObject",300,407,{"name":92,"@type":93},"cksankcs","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-05","2026-09-27",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",13,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What are QIAGEN Plasmid Kits intended for?","Question",{"text":113,"@type":114},"They are intended for molecular biology applications, not for diagnosis, prevention, or treatment of a disease.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How should QIAGEN tips and kit components be stored?",{"text":118,"@type":114},"QIAGEN tips should be stored dry at room temperature (15–25°C). Plasmid kit components are stored at room temperature, while after adding RNase A, Buffer P1 should be stored at 2–8°C.",{"name":120,"@type":111,"acceptedAnswer":121},"Which sections help users follow purification protocols and resolve issues?",{"text":122,"@type":114},"The handbook provides detailed protocols for plasmid/cosmid purification and a Troubleshooting Guide, with additional appendices for agarose gel analysis and buffer composition.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},402463,1790625995,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146,"preview_page_count":4},3985747882755,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","July 2023  \nQIAGEN® Plasmid Purification Handbook  \nQIAGEN Plasmid Mini, Midi, Maxi, Mega, and Giga Kits  \nFor purification of ultrapure, transfection-grade plasmid DNA  \nContents  \nKit Contents................................................................................................................ 3  \nShipping and Storage ................................................................................................. 5  \nIntended Use .............................................................................................................. 5  \nSafety Information ....................................................................................................... 6  \nQuality Control........................................................................................................... 6  \nIntroduction ................................................................................................................ 7  \nPrinciple and procedure .................................................................................... 7  \nEquipment and Reagents to Be Supplied by User ............................................................ 9  \nImportant Notes ........................................................................................................ 10  \nProtocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Mini Kit .............. 18  \nProtocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Midi and Maxi Kits.............................................................................................................................. 24  \nProtocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Mega and Giga Kits.............................................................................................................................. 31  \nProtocol: Very Low-Copy Plasmid/Cosmid Purification Using QIAGEN-tip 100 or QIAGEN-tip 500 ...................................................................................................... 40  \nTroubleshooting Guide .............................................................................................. 48  \nAppendix A: Agarose Gel Analysis of the Purification Procedure .................................... 54  \nAppendix B: Composition of Buffers ............................................................................ 58  \nOrdering Information ................................................................................................ 61  \nDocument Revision History ......................................................................................... 66  \nKit Contents  \nQIAGEN Plasmid Kit  \nCatalog no.  \nMini (25) 12123  \nMini (100) 12125  \nMidi (25) 12143  \nMidi (100) 12145  \nMaxi (10) 12162  \nMaxi (25) 12163  \nMaxi (100) 12165  \nQIAGEN-tip 20  \nQIAGEN-tip 500  \nBuffer P2  \nBuffer QBT  \nBuffer QF  \nLyseBlue®  \n25  \n–  \n20 mL  \n60 mL  \n30 mL  \n20 µL  \n100  \n–  \n2 x 20 mL  \n2 x 60 mL  \n140 mL  \n2 x 20 µL  \n–  \n–  \n110 mL  \n2 x 60 mL  \n200 mL  \n110 µL  \n–  \n–  \n500 mL  \n500 mL  \n4 x  \n140 mL  \n500 µL  \n–  \n10  \n110 mL  \n2 x 60 mL  \n200 mL  \n110 µL  \n–  \n25  \n2 x 150 mL  \n2 x 200 mL  \n510 mL  \n2 x  \n150 µL  \n–  \n–  \n4 x 25  \n8 x 150 mL  \n8 x 150 mL  \n8 x 200 mL  \n4 x 510 mL  \n8 x 15 mg  \n8 x 150 µL  \nQIAGEN-tip 100  \n–  \n–  \n25  \n100  \n–  \n–  \nBuffer P1  \n20 mL  \n2 x 20 mL  \n110 mL  \n500 mL  \n110 mL  \n2 x 150 mL  \n8 x 150 mL  \nBuffer P3  \n20 mL  \n2 x 20 mL  \n110 mL  \n500 mL  \n110 mL  \n2 x 150 mL  \nBuffer QC  \n2 x 60 mL  \n2 x 240 mL  \n3 x 205 mL  \n5 x 500 mL  \n3 x 240 mL  \n4 x 500 mL  \n16 x 500 mL  \nRNase A*  \n2 mg  \n2 x 2 mg  \n11 mg  \n50 mg  \n11 mg  \n2 x 15 mg  \nQuick-Start Protocol  \n1  \n1  \n1  \n1  \n1  \n1  \n1  \n* Provided in a 10 mg/mL or 100 mg/mL solution.  \nQIAGEN Plasmid Kit  \nCatalog no.  \nMega (5) 12181  \nMega (25) 12183  \nGiga (5) 12191  \nPlasmid Buffer Set 19046  \nQIAGEN-tip 2500  \n5  \n25  \n–  \n–  \n\n| QIAGEN-tip 10000 | – | – | 5 | – |\n| --- | --- | --- | --- | --- |\n| Buffer P1 | 2 x 150 mL | 3 x 500 mL | 3 x 25","cbCairUfRNeNC9Kv","https://ap.wps.com/l/cbCairUfRNeNC9Kv","pdf",1956154,68,"English","# Contents\n## Kit Contents\n## Shipping and Storage\n## Intended Use\n## Safety Information\n## Quality Control\n## Introduction\n## Principle and procedure\n## Equipment and Reagents to Be Supplied by User\n## Important Notes\n## Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Mini Kit\n## Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Midi and Maxi Kits\n## Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Mega and Giga Kits\n## Protocol: Very Low-Copy Plasmid/Cosmid Purification Using QIAGEN-tip 100 or QIAGEN-tip 500\n## Troubleshooting Guide\n## Appendix A: Agarose Gel Analysis of the Purification Procedure\n## Appendix B: Composition of Buffers\n## Ordering Information\n## Document Revision History","[{\"question\":\"What are QIAGEN Plasmid Kits intended for?\",\"answer\":\"They are intended for molecular biology applications, not for diagnosis, prevention, or treatment of a disease.\"},{\"question\":\"How should QIAGEN tips and kit components be stored?\",\"answer\":\"QIAGEN tips should be stored dry at room temperature (15–25°C). Plasmid kit components are stored at room temperature, while after adding RNase A, Buffer P1 should be stored at 2–8°C.\"},{\"question\":\"Which sections help users follow purification protocols and resolve issues?\",\"answer\":\"The handbook provides detailed protocols for plasmid/cosmid purification and a Troubleshooting Guide, with additional appendices for agarose gel analysis and buffer composition.\"}]","QIAGEN Plasmid Purification Handbook - Plasmid Mini, Midi, Maxi, Mega, and Giga Kits - For purification of ultrapure, transfection-grade plasmid DNA | PDF",1790530739,171]