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The procedure supports different prep scales with rapid runtimes, uses QIAfilter cartridges for efficient lysate clearing, and yields highly concentrated DNA ready for immediate use. Kits deliver high yields with consistent performance, low endotoxin levels for reliable transfection results, and include LyseBlue to help visualize optimal lysis and neutralization for fewer handling errors.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/qiagen-plasmid-plus-kits-ultrafast-plasmid-dna-purification-for-transfection-grade-applications/402457/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/qiagen-plasmid-plus-kits-ultrafast-plasmid-dna-purification-for-transfection-grade-applications/402457.png","ImageObject",300,407,{"name":92,"@type":93},"asda1da","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-31","2026-09-27",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What makes QIAGEN Plasmid Plus Kits fast for large-scale plasmid preparation?","Question",{"text":112,"@type":113},"The workflow uses a vacuum-based, bind-wash-elute procedure designed by QIAGEN Plasmid Plus spin-column chemistry. Prep runtimes are reported as 20 minutes for Midi/Maxi and about 40–50 minutes for Mega/Giga.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does the kit support parallel processing of multiple samples?",{"text":117,"@type":113},"QIAfilter cartridges enable rapid lysate clearing, and a vacuum manifold draws cleared lysate and wash buffers through the columns. This supports convenient processing of multiple samples in parallel.",{"name":119,"@type":110,"acceptedAnswer":120},"Why are low endotoxin levels important for transfection experiments?",{"text":121,"@type":113},"Endotoxins can trigger nonspecific activation of immune responses, which can skew transfection readouts and reduce reproducibility. The kit uses an endotoxin wash buffer designed to reduce endotoxin levels to below 1 EU/µg.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},402457,1790675323,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":19,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":52,"preview_page_count":4},3985747861852,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","QIAGEN® Plasmid Plus Kits  \nFor the fastest and most convenient purification of transfection-grade plasmid DNA suitable for all applications  \nQIAGEN Plasmid Plus Kits provide a novel patent-pending method for extremely fast and easy large-scale plasmid preparation. The procedure can be performed in 20 (Midi and Maxi), 40 (Mega), or 50 minutes (Giga) using a vacuum and centrifuge.  \nBenefits of QIAGEN Plasmid Plus Kits:  \n• Ultrafast procedure for maximum convenience*  \n• Easy vacuum-based parallel processing of up to 24 samples†  \n• Highly concentrated DNA suitable for all applications  \n• Transfection-grade DNA with very low endotoxin levels  \nUltrafast and easy procedure  \nQIAGEN Plasmid Plus Kits provide a convenient method for large-scale plasmid preparation. The design and unique binding chemistry of the QIAGEN Plasmid Plus spin columns allow a simple bind-wash-elute procedure based on a novel chemistry. The resulting highly concentrated DNA is ready for immediate use in subsequent applications. The procedure is extremely fast — 20 minutes for midi/maxi preps and 40–50 minutes for mega/giga preps.  \nConvenient parallel processing using a vacuum-based procedure  \nQIAfilter cartridges provided in QIAGEN Plasmid Plus Kits enable rapidlysate clearing. Loading of the cleared lysate onto the column can be performed in one step, saving time. A vacuum manifold (such as the QIAvac 24 Plus in combination with the QIAvac holder; see Figure 1, next page) is used to draw the cleared lysate and subsequent wash buffers through the QIAGEN Plasmid Plus column. After washing and drying of the membrane, highly concentrated DNA is eluted in low volumes of elution buffer by centrifugation.  \n* 20 minutes for Midi/Maxi and 40–50 minutes for Mega/Giga † 12 for Mega/Giga  \nQIAGEN Plasmid Plus Procedure  \nPelleted bacteria  \nAlkaline lysate  \nClear lysate  \nAdd binding buffer  \nWash  \n(x2)  \nElute  \nBind  \nVacuum  \nWash  \n(x2)  \nVacuum  \nElute  \nPure Pure  \nplasmid DNA plasmid DNA  \nMidi/Maxi  \nWash  \n(x2)  \nVacuum  \nElute  \nPure  \nplasmid DNA  \nMega/Giga  \nSample to Insight  \nFigure 1. QIAGEN Plasmid Plus Mega and Giga columns on the QIAvac 24 Plus.  \nβ-gal activity (U/mg protein)  \nQIA lter Plasmid Mega  \nQIAGEN Plasmid Plus Mega  \nQIAGEN Plasmid Plus Giga  \nFigure 2. QIAGEN Plasmid Plus Mega and Giga Kits deliver the same performance as the QIAfilter Plasmid Mega Kit. Plasmid pCMVβ DNA was prepared using the indicated preparation method. 1.0 x 104 Huh-7 cells per well were transfected using 200 ng plasmid DNA and 2 µl Effectene® Transfection Reagent. Four wells were transfected in parallel per plasmid preparation method. β-galactosidase activity and protein content were determined 48 hours after transfection.  \nµg DNA/ml biomass 7.0  \n6.0  \n5.0  \n4.0  \n3.0  \n2.0  \n1.0  \n0  \nQPP QPP QP Supplier T Supplier S (high-yield) (standard) (standard)  \nConsistent quality and performance  \nQIAGEN Plasmid Plus technology delivers the same performance and quality as anion-exchange technology. The unique binding chemistry of the QIAGEN Plasmid Plus spin columns ensures that highly pure plasmid DNA is obtained every time (Figure 2) .  \nHigh yields  \nQIAGEN Plasmid Plus Kits deliver extremely high yields of pure plasmid DNA free from contaminants such as RNA or genomic DNA, making it suitable for a wide range of downstream applications. Optimized high-yield protocols and extra buffer volumes are provided with the kit, enabling yields from 250 µg (Midi) to 10 mg (Giga) . As seen in Figure 3, QIAGEN Plasmid Plus Kits outperform kits from other suppliers and deliver consistently high yields.  \nHighly concentrated, ready-to-use plasmid DNA  \nThe innovative binding technology and optimized protocol enable elution of DNA in very low buffer volumes, providing highly concentrated DNA, typically >1 µg/µl. No further alcohol precipitation or centrifugation is necessary. The eluted plasmid DNA can be used immediately, even for reactions where a low reaction volume is r","cbCailEsWvW94Or3","https://ap.wps.com/l/cbCailEsWvW94Or3","pdf",280563,"English","# Key advantages\n## Ultrafast purification workflow\n## Convenient parallel processing with vacuum\n# Procedure and workflow\n## Bind-wash-elute chemistry\n## LyseBlue visualization for lysis and neutralization\n# Performance and suitability\n## High yields and concentrated DNA\n## Low endotoxin for sensitive transfection\n# Applications\n## Enzymatic modification and cloning\n## Sequencing and in vitro applications","[{\"question\":\"What makes QIAGEN Plasmid Plus Kits fast for large-scale plasmid preparation?\",\"answer\":\"The workflow uses a vacuum-based, bind-wash-elute procedure designed by QIAGEN Plasmid Plus spin-column chemistry. Prep runtimes are reported as 20 minutes for Midi/Maxi and about 40–50 minutes for Mega/Giga.\"},{\"question\":\"How does the kit support parallel processing of multiple samples?\",\"answer\":\"QIAfilter cartridges enable rapid lysate clearing, and a vacuum manifold draws cleared lysate and wash buffers through the columns. This supports convenient processing of multiple samples in parallel.\"},{\"question\":\"Why are low endotoxin levels important for transfection experiments?\",\"answer\":\"Endotoxins can trigger nonspecific activation of immune responses, which can skew transfection readouts and reduce reproducibility. The kit uses an endotoxin wash buffer designed to reduce endotoxin levels to below 1 EU/µg.\"}]","QIAGEN Plasmid Plus Kits - Ultrafast plasmid DNA purification for transfection-grade applications | PDF",1790530728]