[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-383654-105":59,"doc-detail-383654-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","pttg1-enhances-oncolytic-adenovirus-5-entry-into-pancreatic-adenocarcinoma-cells-by-increasing-cxadr-expression","PTTG1 Enhances Oncolytic Adenovirus 5 Entry into Pancreatic Adenocarcinoma Cells by Increasing CXADR Expression","","Pituitary tumor-transforming gene 1 (PTTG1) is frequently overexpressed in tumors and acts as an oncogene, making it a candidate for targeted therapy. To clarify its role in pancreatic adenocarcinoma (PAAD), TCGA analyses linked high PTTG1 expression with advanced clinical stages and poorer prognosis. Functional studies using CCK-8 showed increased IC50 values for gemcitabine and 5-FU in PTTG1-high PAAD cell lines. TIDE results indicated reduced immune checkpoint blockade efficacy in the PTTG1-high group. Oncolytic adenovirus OAd5 entry and GFP fluorescence were enhanced in PTTG1-high cells and reduced in PTTG1-low cells, correlating with increased CXADR receptor expression; CXADR knockdown prevented further enhancement, supporting a PTTG1→CXADR→OAd5 entry mechanism.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/pttg1-enhances-oncolytic-adenovirus-5-entry-into-pancreatic-adenocarcinoma-cells-by-increasing-cxadr-expression/383654/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/pttg1-enhances-oncolytic-adenovirus-5-entry-into-pancreatic-adenocarcinoma-cells-by-increasing-cxadr-expression/383654.png","ImageObject",300,407,{"name":92,"@type":93},"River Wang","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-25","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":24},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What relationship did TCGA data show between PTTG1 expression and pancreatic cancer outcomes?","Question",{"text":112,"@type":113},"Higher PTTG1 expression was associated with higher clinical stages and worse prognosis in pancreatic cancer.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How did PTTG1-high versus PTTG1-low cells respond to gemcitabine and 5-FU?",{"text":117,"@type":113},"In BxPC-3-PTTG1high and MIA PaCa-2-PTTG1high cells, the IC50 values for gemcitabine and 5-FU were increased compared with PTTG1-low cells.",{"name":119,"@type":110,"acceptedAnswer":120},"How does PTTG1 influence OAd5 entry into pancreatic cancer cells?",{"text":121,"@type":113},"PTTG1 enhanced OAd5 transduction by increasing CXADR expression on the cell surface; when CXADR was knocked down, PTTG1 could not further enhance OAd5 transduction.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},383654,1790452335,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":24,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},1099514067438,"https://ap-avatar.wpscdn.com/avatar/100002539ee87300030?x-image-process=image/resize,m_fixed,w_180,h_180&k=1780474512215547542","viruses   \nArticle  \nPTTG1 Enhances Oncolytic Adenovirus 5 Entry into Pancreatic Adenocarcinoma Cells by Increasing CXADR Expression  \nLu Long 1, Jian Gao 2 and Ruiyang Zhang 1, *  \nCitation: Long, L.; Gao, J.; Zhang, R. PTTG1 Enhances Oncolytic Adenovirus 5 Entry into Pancreatic Adenocarcinoma Cells by Increasing CXADR Expression. Viruses 2023, 15, 1153. [https://doi.org/10.3390/](https://doi.org/10.3390/)[ ](https://doi.org/10.3390/)v15051153  \nAcademic Editor: Giuseppe Portella  \nReceived: 15 April 2023  \nRevised: 7 May 2023  \nAccepted: 9 May 2023  \nPublished: 11 May 2023  \nCopyright: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Department of Clinical Laboratory, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; [longl199084@163.com](longl199084@163.com)  \n2 State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiao Tong University School of Medicine, Shanghai 200032, China; [jian.gao@uni-wh.de](jian.gao@uni-wh.de)  \n* Correspondence: [316012@sh9hospital.org.cn](316012@sh9hospital.org.cn)  \nAbstract: Pituitary tumor-transforming gene 1 (PTTG1) is overexpressed in various types of tumorsand functions as an oncogene; it could also be a potential target in tumor therapy. Meanwhile, the high mortality of pancreatic adenocarcinoma (PAAD) largely depends on the limited effectiveness of therapy. Based on the promising potential of PTTG1 in cancer treatment, we explored the influence of PTTG1 on the treatment of PAAD in this study. The Cancer Genome Atlas Program (TCGA) data showed that higher expression of PTTG1 was associated with higher clinical stages and worse prognosis of pancreatic cancer. In addition, the CCK-8 assay showed that the IC50 of gemcitabine and 5-fluorouracil (5-FU) was increased in BxPC-3-PTTG1high and MIA PaCa-2-PTTG1high cells. The TIDE algorithm indicated that the immune checkpoint blockades' (ICBs) efficiency is poor in the PTTG1 high group. Furthermore, we found that the efficiency of OAd5 was enhanced in BxPC-3-PTTG1high and MIA PaCa-2-PTTG1high cells and poor in BxPC-3-PTTG1low and MIA PaCa-2-PTTG1low cells. We used the OAd5 expressing GFP for transduction. As a result, the fluorescence intensity was enhanced in BxPC-3-PTTG1high and MIA PaCa-2-PTTG1high cells and decreased in BxPC-3-PTTG1low and MIA PaCa-2-PTTG1low cells 24 h after OAd5 transduction. The fluorescence intensity indicated that PTTG1 increased OAd5 entry. The flow cytometry assay showed that OAd5 receptor CXADR expression was enhanced by PTTG1 . PTTG1 failed to further enhance OAd5 transduction in the case of CXADR knockdown. In summary, PTTG1 enhanced OAd5 transduction into pancreatic cancer cells by increasing CXADR expression on the cell surface.  \nKeywords: PTTG1; pancreatic adenocarcinoma; oncolytic adenovirus; CXADR  \n1. Introduction  \nPituitary tumor-transforming gene 1 (PTTG1) regulates sister chromatid separation during mitosis [1] . It also involves several cellular processes, such as DNA damage repair, apoptosis, and metabolism [2] . Furthermore, PTTG1 is overexpressed and oncogenic in many kinds of tumors, such as prostate, breast, liver, lung, and seminoma [3–7] . PTTG1 promotes tumor cell proliferation, invasiveness, epithelial–mesenchymal transition, and angiogenesis [8–10] . Given the critical tumor-promoting role of PTTG1 in tumor progression, it is necessary to comprehensively understand its potential in therapy.  \nPancreatic adenocarcinoma (PAAD) has exceptionally high mortality, with a median survival time of six–nine months and a five-year survival rate of less than 10% due to late diagnosis and limited effectiveness of treatment strate","cbCaiegKXPMkOqBD","https://ap.wps.com/l/cbCaiegKXPMkOqBD","pdf",2554099,17,"English","# Abstract\n# Introduction","[{\"question\":\"What relationship did TCGA data show between PTTG1 expression and pancreatic cancer outcomes?\",\"answer\":\"Higher PTTG1 expression was associated with higher clinical stages and worse prognosis in pancreatic cancer.\"},{\"question\":\"How did PTTG1-high versus PTTG1-low cells respond to gemcitabine and 5-FU?\",\"answer\":\"In BxPC-3-PTTG1high and MIA PaCa-2-PTTG1high cells, the IC50 values for gemcitabine and 5-FU were increased compared with PTTG1-low cells.\"},{\"question\":\"How does PTTG1 influence OAd5 entry into pancreatic cancer cells?\",\"answer\":\"PTTG1 enhanced OAd5 transduction by increasing CXADR expression on the cell surface; when CXADR was knocked down, PTTG1 could not further enhance OAd5 transduction.\"}]","PTTG1 Enhances Oncolytic Adenovirus 5 Entry into Pancreatic Adenocarcinoma Cells by Increasing CXADR Expression | PDF",1790258583,43]