[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-372238-105":59,"doc-detail-372238-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","ptov1-interacts-with-znf449-to-promote-colorectal-cancer-development","PTOV1 interacts with ZNF449 to promote colorectal cancer development","","PTOV1 plays a significant role in multiple human cancers, yet its clinical relevance and biological function in colorectal cancer (CRC) remained unclear. Mining TCGA, NCBI/GEO, and Kaplan–Meier datasets together with CRC tissue and cell analyses showed PTOV1 upregulation and association with poorer survival. ZNF449 physically interacted with PTOV1 and accelerated CRC development. PTOV1/ZNF449 promoted MYC transcription, while disrupting this interaction using TAT-PTOV1 (125–283 aa) inhibited CRC progression in a xenografted mouse model, suggesting PTOV1/ZNF449 as a therapeutic target.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/ptov1-interacts-with-znf449-to-promote-colorectal-cancer-development/372238/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/ptov1-interacts-with-znf449-to-promote-colorectal-cancer-development/372238.png","ImageObject",300,407,{"name":92,"@type":93},"Riley","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-23",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What role does PTOV1 play in colorectal cancer development?","Question",{"text":112,"@type":113},"PTOV1 expression is upregulated in CRC tissues and cells and is linked to shorter survival, indicating an important role in CRC progression.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does ZNF449 affect PTOV1-related CRC biology?",{"text":117,"@type":113},"ZNF449 interacts with PTOV1 and accelerates CRC development in vitro and in vivo, with the PTOV1/ZNF449 complex enhancing MYC transcription.",{"name":119,"@type":110,"acceptedAnswer":120},"Can disrupting PTOV1/ZNF449 reduce CRC progression?",{"text":121,"@type":113},"Yes. The TAT-PTOV1 (125–283 aa) strategy disrupts PTOV1/ZNF449 interaction and inhibits CRC development in an axenografted mouse model.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},372238,1790838706,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},1374391975076,"https://ap-avatar.wpscdn.com/avatar/14000253ca4ec9f6853?x-image-process=image/resize,m_fixed,w_180,h_180&k=1783305029341752051","communications biology Article  \nA Nature Portfolio journal  \n[https://doi.org/10.1038/s42003-025-07930-2](https://doi.org/10.1038/s42003-025-07930-2)  \nPTOV1 interacts with ZNF449 to promote colorectal cancer development  \n Check for updates  \n\n| Zhiyong Wang1,5, Xinwei Qiao2,5, Kaming Xue3, Qianzhi Chen 4  & Anshu Li 1  |  |\n| --- | --- |\n| PTOV1 is recognized to have a significant role in various human cancers, including prostate cancer. However, it remains unclear what its clinical significance and biological role are in colorectal cancer (CRC) . TCGA, NCBI/GEO, and Kaplan–Meier plot database mining provided important clues into the function and clinical importance of PTOV1 in CRC. Western blotting, immunohistochemistry, and immunofluorescence were utilized to discover PTOV1 protein levels in CRC cell lines and tissues. To explore the involvement of PTOV1inthe development ofCRC andthe underlying mechanisms, several in-vitro and in-vivo studies were executed, such as CCK-8assays, colony formation, transwell assays, qRT-PCR, Co-IP, GST pull-down, immunostaining, and mouse xenograft assays. It was shown that PTOV1 expression level was upregulated in the tissues and cells of human CRC. PTOV1 highexpression level was associated with short survival. ZNF449 interacted with PTOV1 and accelerated CRC development in vitro and in vivo. Through Co-IP and GST pull-down studies, the physical interaction of PTOV1/ZNF449 was demonstrated. Furthermore, PTOV1 directly bound ZNF449, and this complex synergistically promoted the transcription of MYC. In addition, the PTOV1/ZNF449 interaction was disrupted by the TAT-PTOV1 (125–283 aa) protein leading to inhibit the CRC development in axenografted mouse model. According to these findings, PTOV1 has an essential role in CRC progression, and PTOV1/ZNF449 interaction could be a possible therapeutic target for CRC. |  |\n| Colorectal cancer(CRC)is considered oneofthe major prevalent malignant cancers, which rise everyyear worldwide, and the health and lives ofhumans are gravely threatened by it1. CRC is still the fourth greatest cause of death globally despite therapeutic advancesin the last decade2. It is urgent that new therapeutic approaches be found for CRC, which is marked by uncontrolled cellular proliferation.\u003Cbr>In a differential display technique for screening of overexpressed genes in prostate cancer, prostate tumor overexpressed-1 (PTOV1) was specified as a new gene and protein. In a 12-exon gene restricted to chromosome 19q13.3, this protein was encoded3. In the carcinoma and intraepithelial neoplasia of prostate, PTOV1 is overexpressed but cannot be detected or expressed at lower levels of healthy prostate cells. In prostate tumors, PTOV1levels were significantly correlatedwith Ki67proliferative index and related to the proteins’ nuclear localization4. As well as co-localizing at the plasma membrane and in the nucleus, PTOV1 reacts with the lipid rafts protein flotillin-1. Under basal conditions, PTOV1 depletion significantly inhibited cell proliferation5. The non-small cell lung cancer cells were | proven as sensitive to chemotherapy when PTOV1 is depleted, as reported by Wu et al.6 revealing the role ofPTOV1 in tumor progression.\u003Cbr>This study explored the impact ofPTOV1on the development ofCRC. PTOV1 expression was overexpressed in human CRC. Mechanistically, PTOV1 was found to interact with ZNF449 to synergistically enhance the transcription of MYC to promote CRC development. In addition, the PTOV1/ZNF449 interaction, which disrupted, decreased the development of xenografted CRC in nude mice.\u003Cbr>Results\u003Cbr>PTOV1 expression is overexpressed in human CRC\u003Cbr>In order to screen specifically highly expressed protein molecules in CRC tumor tissues, we utilized high-throughput sequencing to determine the differences in the protein expression in three paired healthy colorectal and CRC tissues. The findings demonstrated that PTOV1 was particularly highly expressed in tumor specimens (Fig. S1A). To fu","cbCaib2p3OTialVn","https://ap.wps.com/l/cbCaib2p3OTialVn","pdf",13662440,14,"English","# Introduction\n## PTOV1 in human cancers and rationale for CRC\n# Results\n## PTOV1 is overexpressed in human CRC\n## PTOV1/ZNF449 interaction and functional outcomes\n## Disrupting PTOV1/ZNF449 inhibits CRC development\n# Conclusion and therapeutic implications","[{\"question\":\"What role does PTOV1 play in colorectal cancer development?\",\"answer\":\"PTOV1 expression is upregulated in CRC tissues and cells and is linked to shorter survival, indicating an important role in CRC progression.\"},{\"question\":\"How does ZNF449 affect PTOV1-related CRC biology?\",\"answer\":\"ZNF449 interacts with PTOV1 and accelerates CRC development in vitro and in vivo, with the PTOV1/ZNF449 complex enhancing MYC transcription.\"},{\"question\":\"Can disrupting PTOV1/ZNF449 reduce CRC progression?\",\"answer\":\"Yes. The TAT-PTOV1 (125–283 aa) strategy disrupts PTOV1/ZNF449 interaction and inhibits CRC development in an axenografted mouse model.\"}]","PTOV1 interacts with ZNF449 to promote colorectal cancer development | PDF",1790194045,35]