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This study investigates the role of PTEN in regulating the PI3K/AKT pathway and its effects on RCC cell proliferation and migration. RCC and adjacent non-cancer tissues and RCC cell lines were analyzed by RT-qPCR and Western blot, with functional assays using CCK-8 and wound healing. PTEN overexpression suppressed PI3K/AKT signaling, reducing cell viability and migration, highlighting PTEN as a key therapeutic target.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/pten-suppresses-renal-cell-carcinoma-proliferation-and-migration-via-inhibition-of-the-pi3kakt-pathway-research-open-access/364374/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/pten-suppresses-renal-cell-carcinoma-proliferation-and-migration-via-inhibition-of-the-pi3kakt-pathway-research-open-access/364374.png","ImageObject",300,407,{"name":92,"@type":93},"Emma Wilson","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-23",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the research objective of this study?","Question",{"text":112,"@type":113},"To examine PTEN’s role in managing the PI3K/AKT pathway and its impact on RCC cell proliferation and migration.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were PTEN and PI3K/AKT pathway-related changes measured?",{"text":117,"@type":113},"PTEN expression and related protein levels were detected using RT-qPCR and Western blot, followed by pathway activity assessment.",{"name":119,"@type":110,"acceptedAnswer":120},"What were the main findings regarding PTEN and RCC cell behavior?",{"text":121,"@type":113},"PTEN overexpression in 786-O cells inhibited PI3K/AKT signaling activity and decreased both cell viability and migration ability.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},364374,1790536722,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":39,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":46},3848291630094,"https://eur-avatar.wpscdn.com/davatar_085a072bc5b1113ac321206ff7593b45","Xu etal. World Journal of Surgical Oncology (2025) 23:42  \n[https://doi.org/10.1186/s12957-025-03658-9](https://doi.org/10.1186/s12957-025-03658-9)  \nWorld Journal of Surgical Oncology  \nRESEARCH Open Access  \nPTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathway  \nXu Xu1†, Yuan-Yue Tang1†, Xiaohong Liang2†, Wen Luo1, Dong-Mei Jiang1* and Jie Chen1*  \nAbstract  \nBackground Renal cell carcinoma (RCC) is a frequent and aggressive type of kidney cancer with limited therapeutic options. Although phosphatase and tensin homolog (PTEN) have been recognized as a potential tumor suppressor in all kinds of cancers, its function in RCC remains to be thoroughly elucidated.  \nObjective This article was recruited to examine the PTEN’s role in managing the PI3K/AKT pathway and its impact on the RCC cell proliferation and migration.  \nMethods This study collected renal cancer and adjacent non-cancerous tissue samples from our hospital. HK-2 and 786-O cells were used, with 786-O cells divided into control, vector, and oe-PTEN groups. PTEN and related protein levels were detected using RT-qPCR and Western blot. Statistical analyses were performed using the Mann-Whitney U test and Kruskal-Wallis H test. Cell viability and migration were assessed using the CCK-8 assay and wound healing assay. All analyses were conducted with SPSS 22.0 software, with statistical significance defined as p \u003C 0.05.  \nResults RT-qPCR results showed that PTEN expression was significantly increased in RCC tumor tissues compared to normal tissues (p \u003C 0. 01) . However, PTEN mRNA levels were significantly reduced in 786-O cells compared to HK-2 cells (p \u003C 0. 01) . In 786-O cells with low PTEN expression, further induction of PTEN overexpression significantly inhibited PI3K/AKT signaling activity (p \u003C 0. 01), accompanied by decreased cell viability and migration ability. These results indicate that the expression pattern of PTEN in RCC is complex, but its overexpression can exert tumor-suppressive effects by inhibiting the PI3K/AKT signaling pathway.  \nConclusion Our findings demonstrate that PTEN overexpression in RCC cells leads to decreased PI3K/AKT signaling, decreasing cell viability and migration. This study highlights the critical role of PTEN in RCC progression and suggests potential therapeutic targets for intervention.  \nKeywords PTEN, PI3K/AKT pathway, Renal cell carcinoma, Cell proliferation, Cell migration  \n†Xu Xu, Yuan-yue Tang and Xiaohong Liang contributed equally to this work as Co-first author.  \n*Correspondence:  \nDong-Mei Jiang [jiangyan.zhoufang@163.com](jiangyan.zhoufang@163.com)  \nJie Chen  \n[chenjiedoctor@smmu.edu.cn](chenjiedoctor@smmu.edu.cn); [eioa2014@163.com](eioa2014@163.com)  \n1Department of Haematology, The First Affiliated Hospital of Naval Medical University, No.168 Changhai Road, Yangpu District, Shanghai 200433, China  \n2Department of Urology, Fudan University Shanghai Cancer Center, No.  \n270 Dongan Road, Xuhui District, Shanghai 200032, China  \n© The Author(s) 2025, corrected publication 2026. Open Access This article is licensed under a Creative Commons Attribution-NonCommercialNoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly fro","cbCaidMowSAXSVyt","https://ap.wps.com/l/cbCaidMowSAXSVyt","pdf",2084260,"English","# Abstract\n# Background\n# Objective\n# Methods\n# Results\n# Conclusion\n# Keywords\n# Introduction","[{\"question\":\"What is the research objective of this study?\",\"answer\":\"To examine PTEN’s role in managing the PI3K/AKT pathway and its impact on RCC cell proliferation and migration.\"},{\"question\":\"How were PTEN and PI3K/AKT pathway-related changes measured?\",\"answer\":\"PTEN expression and related protein levels were detected using RT-qPCR and Western blot, followed by pathway activity assessment.\"},{\"question\":\"What were the main findings regarding PTEN and RCC cell behavior?\",\"answer\":\"PTEN overexpression in 786-O cells inhibited PI3K/AKT signaling activity and decreased both cell viability and migration ability.\"}]","PTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathway - Research Open Access | PDF",1790157336]