[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-330728-105":59,"doc-detail-330728-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","pseudogene-cspg4p12-inhibits-colorectal-cancer-progression-by-attenuating-epithelial-mesenchymal-transition","Pseudogene CSPG4P12 inhibits colorectal cancer progression by attenuating epithelial-mesenchymal transition","","Colorectal cancer is among the most common malignant cancers. Pseudogenes can function as oncogenes or tumor suppressors in cancer development, yet the role of pseudogene CSPG4P12 in colorectal cancer is unclear. This study assessed whether CSPG4P12 regulates colorectal cancer behavior and explored underlying mechanisms using GEPIA2 differential expression analysis, proliferation and survival assays, migration/invasion assays, and Western blot analysis of EMT markers. CSPG4P12 was downregulated in tumor tissues, and its overexpression inhibited proliferation, invasion, and migration by increasing E-cadherin while reducing vimentin, N-cadherin, and MMP9, suggesting a potential therapeutic target.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/pseudogene-cspg4p12-inhibits-colorectal-cancer-progression-by-attenuating-epithelial-mesenchymal-transition/330728/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/pseudogene-cspg4p12-inhibits-colorectal-cancer-progression-by-attenuating-epithelial-mesenchymal-transition/330728.png","ImageObject",300,407,{"name":92,"@type":93},"Hazel","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-22","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":19},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main aim of the study on CSPG4P12 in colorectal cancer?","Question",{"text":112,"@type":113},"To investigate the potential role of pseudogene CSPG4P12 in colorectal cancer and explore its underlying molecular mechanism.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does CSPG4P12 expression differ between colorectal tumor tissues and adjacent normal tissues?",{"text":117,"@type":113},"Colorectal cancer tissues show lower CSPG4P12 expression than adjacent normal tissues.",{"name":119,"@type":110,"acceptedAnswer":120},"What cellular effects result from overexpressing CSPG4P12 in colorectal cancer cells?",{"text":121,"@type":113},"Overexpression of CSPG4P12 inhibits cell proliferation, invasion, and migration.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},330728,1790254749,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":19,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":44,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},137441390410,"https://ap-avatar.wpscdn.com/avatar/2000252f4ab5702993?_k=1776741390130283984","Brazilian Journal of Medical and Biological Research (2024) 57: e13645, [https://doi.org/10.1590/1414-431X2024e13645](https://doi.org/10.1590/1414-431X2024e13645)  \nISSN 1414-431X Research Article  \n1/9  \nPseudogene CSPG4P12 inhibits colorectal cancer progression by attenuating epithelial-mesenchymal transition  \nQinqin Song 1,2 00 , Hongxue Xu3 00 , Hongjiao Wu3 00 , Jing Dong4 00 , Shanshan Ji4 00 ,  \nXuemei Zhang5 00 , Zhi Zhang4 00 , and Wanning Hu 1,2 00   \n1Department of Oncology, Hebei Medical University, Shijiazhuang, China  \n2Affiliated Tangshan Gongren Hospital, Hebei Medical University, Tangshan, China 3School of Public Health, North China University of Science and Technology, Tangshan, China  \n4Affiliated Tangshan Gongren Hospital, North China University of Science and Technology, Tangshan, China 5College of Life Science, North China University of Science and Technology, Tangshan, China  \nAbstract  \nColorectal cancer is one of the most common malignant cancers. Pseudogenes have been identified as oncogenes or tumor suppressor genes in the development of various cancers. However, the function of pseudogene CSPG4P12 in colorectal cancer remains unclear. Therefore, the aim of this study was to investigate the potential role of CSPG4P12 in colorectal cancer and explore the possible underlying mechanism. The difference of CSPG4P12 expression between colorectal cancer tissues and adjacent normal tissues was analyzed using the online Gene Expression Profiling Interactive Analysis 2 (GEPIA2) database. Cell viability and colony formation assays were conducted to evaluate cell viability. Transwell and wound healing assays were performed to assess cell migration and invasion capacities. Western blot was used to measure the expression levels of epithelial-mesenchymal transition-related proteins. Colorectal cancer tissues had lower CSPG4P12 expression than adjacent normal tissues. The overexpression of CSPG4P12 inhibited cell proliferation, invasion, and migration in colorectal cancer cells. Overexpressed CSPG4P12 promoted the expression of E-cadherin, whereas it inhibited the expression of vimentin, N-cadherin, and MMP9 . These findings suggested that CSPG4P12 inhibits colorectal cancer development and may serve as a new potential target for colorectal cancer.  \nKey words: Colorectal cancer; Pseudogene; CSPG4P12; EMT; Mechanism  \nIntroduction  \nColorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer-related deaths worldwide with an estimated number of 1.88 million new cases and about 915,880 deaths worldwide in 2020 (1) . The initiation, progression, and metastasis of CRC is a multi-step process caused by the accumulation of genetic and epigenetic alterations (2) . In recent years, studies revealed that around 16–17% CRC patients carried pathogenic germline variants (3,4) . Epigenetic modifications, including DNA methylation, histone modifications, long noncoding RNAs (lncRNAs) and microRNAs (miRNAs), play an important role in the development of CRC (2) . During the last decade, due to earlier diagnosis through screening, advances in surgical techniques, and novel targeted therapies, the 5-year relative survival rate for CRC has greatly improved from 50 to 65% (5) .  \nHowever, stage IV CRC patients still have a poor prognosis with 11–15% 5-year survival rate (5) . Therefore, there is an urgent need to explore new therapeutic targets of CRC.  \nPseudogenes usually refer to a DNA segment structurally similar to a gene but lacking coding function. Recently, increasing evidence indicates that pseudogenes are involved in gene regulation and play important roles in the process of tumor progression (6–8) . For example, RP9P promotes colorectal cancer progression by regulating the miR-133a-3p/FOXQ1 axis (9) . FLT1P1 inhibits both VEGFR1 and non-cognate VEGF-A expression, suppressing tumor cell proliferation and xenograft tumor growth (10) .  \nPseudogenes share high sequence homology with their parental g","cbCaiieosZ4WfX6u","https://ap.wps.com/l/cbCaiieosZ4WfX6u","pdf",1085055,"English","# Abstract\n# Introduction\n# Material and Methods","[{\"question\":\"What is the main aim of the study on CSPG4P12 in colorectal cancer?\",\"answer\":\"To investigate the potential role of pseudogene CSPG4P12 in colorectal cancer and explore its underlying molecular mechanism.\"},{\"question\":\"How does CSPG4P12 expression differ between colorectal tumor tissues and adjacent normal tissues?\",\"answer\":\"Colorectal cancer tissues show lower CSPG4P12 expression than adjacent normal tissues.\"},{\"question\":\"What cellular effects result from overexpressing CSPG4P12 in colorectal cancer cells?\",\"answer\":\"Overexpression of CSPG4P12 inhibits cell proliferation, invasion, and migration.\"}]","Pseudogene CSPG4P12 inhibits colorectal cancer progression by attenuating epithelial-mesenchymal transition | PDF",1789989859,23]