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Peony (Paeonia ostii) flowers are used in traditional Chinese medicine for pigment reduction and anti-inflammatory, anti-aging benefits, yet their ability to repair barrier injury remains insufficiently characterized. This study evaluates peony flower extract in tape-stripping-induced mouse barrier damage using imaging, hydration assays, histology, proteomics, qPCR, and immunofluorescence to define mechanisms and therapeutic potential.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/protective-effect-of-peony-paeonia-ostii-flower-extract-against-tape-stripping-induced-skin-barrier-impairment-in-mice/466133/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/protective-effect-of-peony-paeonia-ostii-flower-extract-against-tape-stripping-induced-skin-barrier-impairment-in-mice/466133.png","ImageObject",300,407,{"name":92,"@type":93},"Chumphorn","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-04","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What problem does the study address regarding skin barrier dysfunction?","Question",{"text":112,"@type":113},"The study addresses skin barrier dysfunction that increases transepidermal water loss (TEWL), triggers inflammation, and compromises skin protection.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was peony flower extract evaluated in the mouse model?",{"text":117,"@type":113},"Peony flower extract was tested in a tape-stripping-induced mouse model, using skin surface imaging, hydration measurements, H&E histology, proteomics, qPCR, and immunofluorescence.",{"name":119,"@type":110,"acceptedAnswer":120},"What key mechanisms were identified for skin barrier repair?",{"text":121,"@type":113},"Proteomics indicated cornified envelope formation and tight junction assembly, alongside increased expression of barrier-related proteins including filaggrin, involucrin, loricrin, claudin-1, Tjp1, and occludin.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},466133,1790811368,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},2336475401981,"https://ap-avatar.wpscdn.com/avatar/22000c94efd8d5204d?x-image-process=image/resize,m_fixed,w_180,h_180&k=1786935347598174694","Article  \nProtective Effect of Peony (Paeonia ostii) Flower Extract Against Tape Stripping-Induced Skin Barrier Impairment in Mice  \nRuiying Yang 1,2,†, Jicheng Yang 1,†, Gaiying He 1, Yusheng Zhang 1, Xue Jiang 1, Jiyong Wang 2,*, Hongjun Yang 1,* and Chengxiang Shang 1,2, *  \nAcademic Editor: Andrzej Slominski  \nReceived: 6 August 2025  \nRevised: 12 September 2025  \nAccepted: 16 September 2025  \nPublished: 24 December 2025  \nCopyright: © 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.  \n1 Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China; [15651901275@163.com](15651901275@163.com) (R.Y.); [yjc18143609206@163.com](yjc18143609206@163.com) (J.Y.); [gaiyinghe2012@163.com](gaiyinghe2012@163.com) (G.H.); [yushengzhang271727@foxmail.com](yushengzhang271727@foxmail.com) (Y.Z.); [2112218003@stmail.ujs.edu.cn](2112218003@stmail.ujs.edu.cn) (X.J.)  \n2 China Traditional Chinese Medicine Co., Ltd., Beijing 100089, China  \n* [Correspondence: wangjiyong@sinopharm.com](Correspondence: wangjiyong@sinopharm.com) (J.W.); [hjyang@icmm.ac.cn](hjyang@icmm.ac.cn) (H.Y.); [shangchengxiang@sinopharm.com](shangchengxiang@sinopharm.com) (C.S.); Tel.: +86-010-88468190 (J.W.); +86-10-64089518 (H.Y.);+86-13051851033 (C.S.)  \n† These authors contributed equally to this work.  \nAbstract  \nBackground: Skin barrier dysfunction leads to increased transepidermal water loss (TEWL), inflammation, and compromised skin protection. While Paeonia ostii (peony) flowers are recognized in traditional Chinese medicine for their reducing melanin synthesis, antiinflammatory, and anti-aging effects, their role in repairing skin barrier damage has not been fully explored. Methods: We investigated the therapeutic potential of peony flower extract (PFE) in the tape-stripping-induced mouse model with skin barrier damage. Skin surface imaging, hydration measurements, H&E, proteomics, qPCR, and immunofluorescence were applied to clarify the potential mechanism of PFE in attenuating skin barrier impairment. Results: PFE significantly reduced erythema, TEWL, and edema while restoring epidermal architecture. Proteomics analysis identified cornified envelope formation and tight junction assembly as essential mechanisms in skin barrier repair. It increased the expression of key skin barrier proteins, including filaggrin (Flg), involucrin (Ivl), loricrin (Lor), claudin-1 (Cldn1), tight junction protein 1 (Tjp1), and occludin (Ocln). Conclusions: This study demonstrates that PFE restores skin barrier integrity by upregulating key structural proteins within the cornified envelope and tight junction. These findings suggest that PFE is a promising therapeutic candidate for skin barrier repair, with high potential in translational medicine applications.  \nKeywords: peony flower extract; skin barrier; proteomics; cornified envelope; tight junctions  \n1. Introduction  \nThe skin constitutes the body’s primary defense against external insults—including pathogens, environmental aggressors, and ultraviolet radiation—while concurrently preventing transepidermal water and solute loss [1] . This critical protective function is mediated by a multi-layered barrier system encompassing the stratum corneum, tight junctions, and microbial, chemical, and immune barriers [2,3] . Dysfunction in any component of this system may trigger xerosis, inflammatory responses, and systemic pathological manifestations [4,5] . Specifically, barrier impairment elevates skin permeability, increases  \ntransepidermal water loss (TEWL), reduces hydration levels, elevates pH, and culminatesin clinical sequelae including erythema, desquamation, fissuring, pruritus, and inflammation [6] . Notably, dermatoses such as psoriasis,","cbCaitnL6LVDlm0Q","https://ap.wps.com/l/cbCaitnL6LVDlm0Q","pdf",6197246,18,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n# 1. Introduction","[{\"question\":\"What problem does the study address regarding skin barrier dysfunction?\",\"answer\":\"The study addresses skin barrier dysfunction that increases transepidermal water loss (TEWL), triggers inflammation, and compromises skin protection.\"},{\"question\":\"How was peony flower extract evaluated in the mouse model?\",\"answer\":\"Peony flower extract was tested in a tape-stripping-induced mouse model, using skin surface imaging, hydration measurements, H\\u0026E histology, proteomics, qPCR, and immunofluorescence.\"},{\"question\":\"What key mechanisms were identified for skin barrier repair?\",\"answer\":\"Proteomics indicated cornified envelope formation and tight junction assembly, alongside increased expression of barrier-related proteins including filaggrin, involucrin, loricrin, claudin-1, Tjp1, and occludin.\"}]","Protective Effect of Peony (Paeonia ostii) Flower Extract Against Tape Stripping-Induced Skin Barrier Impairment in Mice | PDF",1790770625,45]