[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-332282-105":59,"doc-detail-332282-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","prostate-specific-membrane-antigen-expression-in-a-syngeneic-breast-cancer-mouse-model","Prostate Specific Membrane Antigen Expression in a Syngeneic Breast Cancer Mouse Model","","Purpose: Validate and characterize prostate specific membrane antigen (PSMA) expression in murine breast cancer models to support PSMA-targeted therapies and imaging, especially for improving breast conserving surgery margins. Methods: Triple negative breast cancer 4T1 and human breast cancer lines were implanted in BALB/c mice and imaged with PSMA-1-Pc413; tumor-to-background ratios were computed. Immunohistochemistry and RT-PCR assessed PSMA localization and correlation with CD31. Results showed selective uptake in tumors and metastases with localization to tumor neovasculature. Conclusion: The syngeneic 4T1 model supports PSMA as a promising oncogenic target for targeted imaging and therapy.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/prostate-specific-membrane-antigen-expression-in-a-syngeneic-breast-cancer-mouse-model/332282/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/prostate-specific-membrane-antigen-expression-in-a-syngeneic-breast-cancer-mouse-model/332282.png","ImageObject",300,407,{"name":92,"@type":93},"Liam","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-07","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the primary goal of the study?","Question",{"text":112,"@type":113},"To validate and characterize PSMA expression in murine breast cancer models, enabling development of PSMA-targeted therapies and imaging approaches.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were the mouse tumors imaged and quantified?",{"text":117,"@type":113},"Murine 4T1 and human breast cancer cell lines were implanted into BALB/c mice and imaged with the PSMA targeted theranostic agent PSMA-1-Pc413; tumor-to-background ratios were calculated to confirm selective uptake.",{"name":119,"@type":110,"acceptedAnswer":120},"What does the study show about where PSMA is expressed?",{"text":121,"@type":113},"PSMA was consistently localized on tumor neovasculature, with no expression in tumor cells and surrounding normal tissues, supported by RT-PCR and PSMA-CD31 co-staining.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},332282,1790073305,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},8796095461564,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Molecular Imaging and Biology (2024) 26:714–728 [https://doi.org/10.1007/s1](https://doi.org/10.1007/s1) 1307-024-01920-2  \nProstate Specific Membrane Antigen Expression in a Syngeneic Breast Cancer Mouse Model  \nAditi A. Shirke1 · Jing Wang2 · Gopolakrishnan Ramamurthy2 · Arpan Mahanty2 · Ethan Walker1 · Lifang Zhang2 · Abhiram Panigrahi2 · Xinning Wang1 · James P. Basilion1,2  \nReceived: 18 December 2023 / Revised: 9 April 2024 / Accepted: 22 April 2024 / Published online: 17 May 2024 © The Author(s) 2024  \nAbstract  \nPurpose Prostate specific membrane antigen (PSMA) has been studied in human breast cancer (BCa) biopsies, however, lack of data on PSMA expression in mouse models impedes development of PSMA-targeted therapies, particularly in improving breast conserving surgery (BCS) margins. This study aimed to validate and characterize the expression of PSMA in murine BCa models, demonstrating that PSMA can be utilized to improve therapies and imaging techniques.  \nMethods Murine triple negative breast cancer 4T1 cells, and human cell lines, MDA-MB-231, MDA-MB-468, implanted into the mammary fat pads of BALB/c mice, were imaged by our PSMA targeted theranostic agent, PSMA-1-Pc413, and tumor to background ratios (TBR) were calculated to validate selective uptake. Immunohistochemistry was used to correlate PSMA expression in relation to CD31, an endothelial cell biomarker highlighting neovasculature. PSMA expression was also quantified by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) .  \nResults Accumulation of PSMA-1-Pc413 was observed in 4T1 primary tumors and associated metastases. Average TBR of 4T1 tumors were calculated to be greater than 1.5—ratio at which tumor tissues can be distinguished from normal structures—at peak accumulation with the signal intensity in 4T1 tumors comparable to that in high PSMA expressing PC3-pip tumors. Extraction of 4T1 tumors and lung metastases followed by RT-PCR analysis and PSMA-CD31 co-staining shows that PSMA is consistently localized on tumor neovasculature with no expression in tumor cells and surrounding normal tissues. Conclusion The selective uptake of PSMA-1-Pc413 in these cancer tissues as well as the characterization and validation of PSMA expression on neovasculature in this syngeneic 4T1 model emphasizes their potential for advancements in targeted therapies and imaging techniques for BCa. PSMA holds great promise as an oncogenic target for BCa and its associated metastases.  \nKeywords Animal model supporting targeted imaging · Breast cancer · Cancer biomarker · Molecular imaging · Molecular imaging contrast agents · Prostate cancer · Prostate specific membrane antigen expression · Triple negative breast cancer  \n* Aditi A. Shirke[aas151@case.edu](aas151@case.edu)  \n* Xinning Wang [xxw171@case.edu](xxw171@case.edu)  \n* James P. Basilion[jxb206@case.edu](jxb206@case.edu)  \n1 Department of Biomedical Engineering, Case Western Reserve University, 11100 Euclid Ave, Wearn Building B-49, Cleveland, OH 44106, USA  \n2 Department of Radiology, Case Western Reserve University, 11100 Euclid Ave, Wearn Building B-49, Cleveland, OH 44106, USA  \nAbbreviations  \nATP Adenosine triphosphate  \nBCa Breast cancer  \nBCS Breast conserving surgery  \nBLI Bioluminescence imaging  \ncDNA Complementary DNA  \nDMEM Dulbecco’s modified eagle medium ER Estrogen receptor  \nFBS Fetal bovine serum  \nFDA United States Food and Drug Administration  \nFIGS Fluorescence image guided surgery  \nFOLH1 Folate hydrolase – 1  \nGTEx Genotype-tissue expression  \nH&E Hematoxylin and eosin staining  \nHER2 Human endothelial growth factor 2  \nHUVEC  \nIC  \nIHC  \nIV  \nLNCaP  \nMC  \nNu  \nOCT  \nPBS  \nPCa  \nPCC  \nPDT  \nPR  \nPS  \nPSMARNA-SEQ RT-PCR  \nROI  \nTCGA  \nTIME  \nTNBC  \nUCSC  \nVEGF  \nHuman vascular endothelial cell Immunocompetent mice Immunohistochemistry  \nIntravenous  \nLymph node carcinoma of the prostate cells Manders coefficient Immunocompromised /athymic/ nude mice Optimal cutting temperature  \nPhosphate-buffe","cbCaioTXNbN7zDbH","https://ap.wps.com/l/cbCaioTXNbN7zDbH","pdf",4963347,15,"English","# Abstract\n## Purpose\n## Methods\n## Results\n## Conclusion\n# Introduction","[{\"question\":\"What is the primary goal of the study?\",\"answer\":\"To validate and characterize PSMA expression in murine breast cancer models, enabling development of PSMA-targeted therapies and imaging approaches.\"},{\"question\":\"How were the mouse tumors imaged and quantified?\",\"answer\":\"Murine 4T1 and human breast cancer cell lines were implanted into BALB/c mice and imaged with the PSMA targeted theranostic agent PSMA-1-Pc413; tumor-to-background ratios were calculated to confirm selective uptake.\"},{\"question\":\"What does the study show about where PSMA is expressed?\",\"answer\":\"PSMA was consistently localized on tumor neovasculature, with no expression in tumor cells and surrounding normal tissues, supported by RT-PCR and PSMA-CD31 co-staining.\"}]","Prostate Specific Membrane Antigen Expression in a Syngeneic Breast Cancer Mouse Model | PDF",1789997632,38]