[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-364939-105":59,"doc-detail-364939-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","proof-of-concept-study-on-differentiating-metastatic-brain-samples-by-their-originating-organ-using-multimodal-autofluorescence-spectroscopy","Proof of concept study on differentiating metastatic brain samples by their originating organ using multimodal autofluorescence spectroscopy","","Brain metastases are the most frequent central nervous system tumors and can be diagnosed before the primary cancer site is identified. Accurate determination of the originating organ is important because it guides treatment choices and improves survival estimates. Existing work shows endogenous fluorescence can distinguish metastasis from controls and reveal optical signatures. This proof-of-concept study uses deep ultraviolet and near-infrared excitation to assess whether endogenous fluorescence characterizes brain metastasis origin and highlights heterogeneity across metastatic sub-types.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/proof-of-concept-study-on-differentiating-metastatic-brain-samples-by-their-originating-organ-using-multimodal-autofluorescence-spectroscopy/364939/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/proof-of-concept-study-on-differentiating-metastatic-brain-samples-by-their-originating-organ-using-multimodal-autofluorescence-spectroscopy/364939.png","ImageObject",300,407,{"name":92,"@type":93},"Genevieve","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-03","2026-09-23",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why is identifying the originating organ of brain metastasis clinically important?","Question",{"text":112,"@type":113},"Accurately determining the primary cancer origin helps guide therapeutic options, which depend on lesion number, location, size, and severity, and it influences survival estimates.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What role does endogenous fluorescence play in differentiating brain metastases?",{"text":117,"@type":113},"Endogenous fluorescence of brain tissue has shown the capability to differentiate metastatic tissue from controls and to identify specific optical signatures.",{"name":119,"@type":110,"acceptedAnswer":120},"What imaging/excitation strategy does this proof-of-concept study evaluate?",{"text":121,"@type":113},"The study investigates endogenous fluorescence analysis using deep ultraviolet and near-infrared excitation to characterize metastasis origin and highlight heterogeneity among metastasis sub-types.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},364939,1790419290,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":41},1374391974585,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nProof of concept study on differentiating metastatic brain samples by their originating organ using multimodal autofluorescence spectroscopy  \nCécile Rimbault1, Bertrand Devaux2,3,4,6, Hussein Mehidine1, Mira Sibai1, Frédéric Jamme5, Matthieu Réfrégiers5,7 & DarineAbi Haidar1,8􀀍  \nBrain metastasis are the most frequent central nervous system tumors that usually occur during systemic malignancy. However, they can be diagnosed before the primary cancer site. The number of brain and systemic metastases and the identification of the primary cancer can favor specific treatments and impacts survival estimates. During surgery, intraoperative identification of metastatic tissue may help the surgical strategy and postoperative adjuvant treatment. The study of endogenous fluorescence of the brain tissue has already demonstrated its capability to differentiate metastasis tissue from control and identify specific optical signatures. In this study, based on deep ultraviolet and near-infrared excitation, we investigated for the first time the potentiality of the endogenous fluorescence analysis to characterize brain metastasis origin and to highlight the heterogeneity of molecular components and properties according to the metastasis sub-types, This study gives thus a glimpse of the possibility to find the primary cancer site from the secondary tumor.  \nKeywords Autofluorescence, SHG, Brain metastasis, Spectral analysis, Phasor FLIM  \nBrain tumors are usually classified according to their cellular types. Two main groups can be distinguished: primary tumors and secondary ones, namely metastasis. While primary tumors such as glioma and meningioma, develop within the central nervous system (CNS), metastasis derive from cancer cells that originate from a malignancy outside the CNS and migrate to the brain, principally via the bloodstream1,2. Contrary to a primary brain tumor, metastasis present either as a single tumor mass or as multiple tumors in different locations in the CNS3. The most common cancers that metastasize to the brain include lung cancer (50%), breast cancer (15–19%), and skin melanoma (10%), making up 70–80% of all brain metastases1. CNS metastatic tumors are 1.0 to 1.5 times more frequently diagnosed than primary brain tumors4, but the latter have a poorer prognosis with lower survival rates5. Some metastases can appear many years after discovering the originating primary cancer while others can appear even before detecting the originating primary cancer3. Usually, the therapeutic options for brain metastases involve a combination of surgery, radiosurgery, chemotherapy, or immunotherapy, targeting both the metastatic brain tumor and primary cancer. Therefore, the therapeutic options depend on the number, location, size, and severity of brain lesions and the primary cancer as well6. Hence, accurately identifying the primary cancer from which a brain metastasis develops is necessary information to guide the therapeutic options7.  \nDetermining the origin of brain metastasis is not a new challenge. Solving this problem could be crucial in future translational research identifying diagnostic or therapeutic targets that could disrupt or revert the growth of brain metastasis7,8. Several studies tried to correlate the spatial distribution of metastases with the primary cancer location7–9. Schroeder et al. reported, based on a large panel of brain metastastatic tissue types (lung,  \n1Université Paris-Saclay, CNRS/IN2P3, IJCLab, Orsay F-91405, France. 2Université Paris Cité – Faculté de Médecine Paris Descartes, Paris 75006, France. 3Service de Neurochirurgie, Hôpital Lariboisière, Paris 75010, France. 4Service de Neurochirurgie, Pôle Neurosciences, GHU-Paris, Paris 75014, France. 5Synchrotron SOLEIL, DISCO Beamline, Gif-sur-Yvette 91192, France. 6Department, GHU Paris-Psychiatrie et Neurosciences, Sainte-Anne Hospital, Paris, France. 7Centre de Biophysique ","cbCaiswjxJLWDejl","https://ap.wps.com/l/cbCaiswjxJLWDejl","pdf",2372503,12,"English","# Background\n## Brain metastases and clinical relevance\n## Need to identify primary origin\n# Prior research and rationale\n## Spatial distribution and predictive modeling\n## Autofluorescence-based tissue discrimination\n# Study aim and approach\n## Multimodal autofluorescence spectroscopy with UV and NIR excitation","[{\"question\":\"Why is identifying the originating organ of brain metastasis clinically important?\",\"answer\":\"Accurately determining the primary cancer origin helps guide therapeutic options, which depend on lesion number, location, size, and severity, and it influences survival estimates.\"},{\"question\":\"What role does endogenous fluorescence play in differentiating brain metastases?\",\"answer\":\"Endogenous fluorescence of brain tissue has shown the capability to differentiate metastatic tissue from controls and to identify specific optical signatures.\"},{\"question\":\"What imaging/excitation strategy does this proof-of-concept study evaluate?\",\"answer\":\"The study investigates endogenous fluorescence analysis using deep ultraviolet and near-infrared excitation to characterize metastasis origin and highlight heterogeneity among metastasis sub-types.\"}]","Proof of concept study on differentiating metastatic brain samples by their originating organ using multimodal autofluorescence spectroscopy | PDF",1790159483]