[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-375620-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-375620-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","prognostic-significance-and-immune-characteristics-of-gpr27-in-gastric-cancer","Prognostic significance and immune characteristics of GPR27 in gastric cancer","","Gastric cancer is a common digestive-system malignancy in which immunotherapy remains a limited option for advanced patients to extend survival. This study identifies G-protein-coupled receptor 27 (GPR27) as a molecular target by integrating bioinformatic analyses of GPR27 transcription and methylation profiles. Survival associations were evaluated alongside immune cell infiltration, immune marker expression, and single-cell expression patterns. Results show reduced GPR27 mRNA in gastric cancer tissues, improved survival with GPR27 down-regulation, strong prognostic value of specific CpG methylation, and predominant expression in macrophages, supporting GPR27 as a prognostic biomarker with immunomodulatory relevance.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/prognostic-significance-and-immune-characteristics-of-gpr27-in-gastric-cancer/375620/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/prognostic-significance-and-immune-characteristics-of-gpr27-in-gastric-cancer/375620.png","ImageObject",300,407,{"name":42,"@type":43},"Aria","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-24","2026-09-24",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",7,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What was the main goal of this research on GPR27 in gastric cancer?","Question",{"text":63,"@type":64},"To determine whether GPR27 transcription and DNA methylation are associated with gastric cancer prognosis and to clarify how GPR27 relates to the immune microenvironment and immune cell infiltration.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How do GPR27 transcription and methylation relate to patient survival?",{"text":68,"@type":64},"GPR27 mRNA expression is lower in gastric cancer tissues, and down-regulation of GPR27 transcription is associated with better survival. Specific methylation sites, including cg03024619, show the strongest prognostic signal.",{"name":70,"@type":61,"acceptedAnswer":71},"Which immune cells are GPR27 mainly associated with?",{"text":72,"@type":64},"Single-cell analysis indicates that GPR27 is mainly expressed in macrophages, and bulk expression levels correlate with immune cell infiltration and immune markers.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},375620,1790269646,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,109,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":107,"slug":108},40,"healthcare",{"id":110,"doc_module":4,"doc_module_name":25,"category_name":111,"show_sort_weight":112,"slug":113},8,"Research & Report",30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":55,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145},2336464648322,"https://ap-avatar.wpscdn.com/avatar/2200025388227c56fec?_k=1778556882303663488","[www.aging-us.com](www.aging-us.com) AGING 2023, Vol. 15, No. 17  \nResearch Paper  \nPrognostic significance and immune characteristics of GPR27 in gastric cancer  \nJun Pan1, Yuanjun Gao1  \n1 Department of Gastroenterology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei, China  \nCorrespondence to: Yuanjun Gao; email: [gastrogaoyuanjun@163.com](gastrogaoyuanjun@163.com), [https://orcid.org/0009-0002-9990-7974](https://orcid.org/0009-0002-9990-7974)[ ](https://orcid.org/0009-0002-9990-7974)[Keywords:](Keywords: gastric cancer)[ gastric cancer](Keywords: gastric cancer), [G-protein-coupled](G-protein-coupled) receptor 27, immunotherapy, survival  \nReceived: May 19, 2023 Accepted: August 21, 2023 Published: September 12, 2023  \nCopyright: © 2023 Pan and Gao. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.  \nABSTRACT  \nGastric cancer (GC) is one of the most typical cancerous neoplasms occurring in the digestive system. For advanced GC, immunotherapy is the final option for them to prolong survival time. Hence, we aimed to identify new molecular targets to enhance the immunotherapy response in GC individuals. Then we applied bioinformatic analysis to explore the expression profiles of G-protein-coupled receptor 27 (GPR27) transcription and GPR27 methylation. The associations between survival of GC patients and GPR27 transcription and methylation were then analyzed. We also studied the link between GPR27 expression and levels of immune cell infiltration. Finally, we gained insights into the prognostic role of GPR27 protein in 97 cases of GC individuals. According to datasets gained from TCGA, GPR27 mRNA is expressed lower in GC tissues. Down-regulation of GPR27 transcription was related with better survival in GC individuals, and GPR27 cg03024619 had the most significant prognostic value (HR=0.553, P\u003C0.0001) . In addition, the expression level of GPR27 has a clear interaction with immune cells' infiltration and their markers. Single-cell analysis displayed that GPR27 is mainly expressed in macrophages. Finally, down-regulation of GPR27 protein was observed in GC tissues and correlated with better survival outcomes. GPR27 can serve as an important prognostic biomarker and exert an immunomodulatory role in GC. Our findings highlight the significance of GPR27 in a variety of cancers, including GC, and provide clues for a better understanding of GPR27 from bioinformatics and clinically validated perspective.  \nINTRODUCTION  \nGastric cancer (GC) is one of the most typical cancerous neoplasms occurring in the digestive system, and it represents the second most well-known reason for cancer death worldwide [1] . The common risk factors for GC include the presence of Helicobacter pylori, genetic susceptibility and diets high in nitrates and nitrites [2] . Surgical resection, systemic chemotherapy, targeted therapy, and radiotherapy are all proven to be efficient in GC treatments [3], but the long-term survival outcomes of GC sufferers are far from satisfactory, especially in patients with advanced GC. For advanced GC patients, immunotherapy is the final option for them to prolong survival time [4, 5], but fewer individuals could benefit from this novel therapy.  \nHence, identification of new molecular targets to enhance the immunotherapy response in GC individuals is an urgent need.  \nG-protein-coupled receptors (GPCRs) are a superfamily with more than 800 members, and some of them are not well studied [6] . G-protein-coupled receptor 27 (GPR27) is a newly identified component of GPCRs super-family. A previous study reported that GPR27 is implicated in key physiological functions, such as energy metabolism, and insulin secretion and regulation, and neuronal plasticity [7] . Recent studies have linked GPR27 to the development an","cbCaioXaBqvh7WKt","https://ap.wps.com/l/cbCaioXaBqvh7WKt","pdf",3703301,23,"English","# Abstract\n# Introduction\n# Materials and Methods\n## Xena Shiny\n## GEPIA 2\n## MethSurv\n## cBioPortal","[{\"question\":\"What was the main goal of this research on GPR27 in gastric cancer?\",\"answer\":\"To determine whether GPR27 transcription and DNA methylation are associated with gastric cancer prognosis and to clarify how GPR27 relates to the immune microenvironment and immune cell infiltration.\"},{\"question\":\"How do GPR27 transcription and methylation relate to patient survival?\",\"answer\":\"GPR27 mRNA expression is lower in gastric cancer tissues, and down-regulation of GPR27 transcription is associated with better survival. Specific methylation sites, including cg03024619, show the strongest prognostic signal.\"},{\"question\":\"Which immune cells are GPR27 mainly associated with?\",\"answer\":\"Single-cell analysis indicates that GPR27 is mainly expressed in macrophages, and bulk expression levels correlate with immune cell infiltration and immune markers.\"}]","Prognostic significance and immune characteristics of GPR27 in gastric cancer | PDF",1790215538,58]