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Six Lactiplantibacillus plantarum strains isolated from Mediterranean fruits were selected under roseoflavin pressure to obtain spontaneous riboflavin-overproducing phenotypes. The top strain (Lp 187_B2, 6 mg/L) showed antibacterial activity, organic acid production, antibiotic resistance, and resilience under digestive stress using an in vitro gut model. As a postbiotic, it increased TEER in Caco-2 monolayers and reduced TNF-α release in a Caco-2/THP-1 inflamed co-culture system. Preliminary findings support both probiotic and postbiotic applications, pending in vivo validation.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/probiotics-and-antimicrobial-proteins-postbiotic-potential-of-newly-isolated-riboflavin-overproducing-lactiplantibacillus-plantarum-strains/514638/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/probiotics-and-antimicrobial-proteins-postbiotic-potential-of-newly-isolated-riboflavin-overproducing-lactiplantibacillus-plantarum-strains/514638.png","ImageObject",300,407,{"name":92,"@type":93},"miaandmiso","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-10-06",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"How were riboflavin-overproducing Lactiplantibacillus plantarum strains obtained?","Question",{"text":112,"@type":113},"Six L. plantarum strains from Mediterranean fruits were exposed to roseoflavin to select spontaneous riboflavin-overproducing phenotypes.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What main properties were evaluated for the best-performing strain?",{"text":117,"@type":113},"The top strain was characterized for antibacterial activity, organic acid production, antibiotic resistance, and survival under digestive stresses using an in vitro gut model.",{"name":119,"@type":110,"acceptedAnswer":120},"What effects did the postbiotic Lp 187_B2 show on the intestinal barrier?",{"text":121,"@type":113},"Lp 187_B2 postbiotics significantly increased transepithelial electrical resistance (TEER) in Caco-2 monolayers and inhibited TNF-α release in a Caco-2/THP-1 co-culture model mimicking inflamed bowel.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},514638,1791396541,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},7971478544854,"https://ap-avatar.wpscdn.com/davatar_994ba38a5ba835b3df7d355c54d3ed8d","Probiotics and Antimicrobial Proteins  \n[https://doi.org/10.1007/s12602-025-10538-x](https://doi.org/10.1007/s12602-025-10538-x)  \nPostbiotic Potential of Newly Isolated Riboflavin‑Overproducing Lactiplantibacillus plantarum Strains  \nAngela Scauro1 · Maria Teresa Rocchetti2 · Mario Soccio1 · Barbara la Gatta1 · Maria Teresa Liberatore1 · Nicola De Simone3 · Giuseppe Spano1 · Daniela Fiocco2 · Pasquale Russo4  \nAccepted: 11 April 2025 © The Author(s) 2025  \nAbstract  \nLactic acid bacteria (LAB) are food-grade microorganisms able to produce and release bioactive compounds of interest to human health. Some LAB strains can synthesize vitamin B2, i.e., riboflavin, a micronutrient essential for cellular metabolism. In this work, six Lactiplantibacillus plantarum isolated from fruits of the Mediterranean area were exposed to the selective pressure of roseoflavin in order to select spontaneous riboflavin-overproducing phenotypes. The best strains, as determined by the level of riboflavin produced, were characterized for some basic probiotic features, including antibacterial activity, production of organic acids, antibiotic resistance, and survival under digestive stresses using an in vitro gut model. The strain L. plantarum Lp 187_B2, which produced the highest riboflavin level (6 mg/L), exhibited good resistance togastro-intestinal stress and a relevant capacity to antagonize undesired bacteria, was selected for additional investigations to assess its capacity to protect intestinal homeostasis. When used as a postbiotic, Lp 187_B2 significantly increased transepithelial electrical resistance (TEER) in Caco-2 cell monolayers, an in vitro model of the intestinal barrier. Moreover, in a Caco-2/THP-1 co-culture system, mimicking the inflamed bowel, Lp 187_B2 postbiotics significantly inhibited the release of TNF-α by macrophages, thus pointing to gut-barrier strengthening and potential anti-inflammatory properties. Though a validation in vivo is required, our preliminary results indicate that L. plantarum Lp 187_B2 could be successfully applied as both probiotic and postbiotic formulations for improving human health.  \nKeywords Gut barrier integrity · TEER · Inflammatory cytokine · Vitamin-producing probiotics · Immunomodulation · Intestinal inflammation  \n* Angela Scauro [angela.scauro@unifg.it](angela.scauro@unifg.it)  \n* Daniela Fiocco [daniela.fiocco@unifg.it](daniela.fiocco@unifg.it)  \n1 Department of Sciences of Agriculture, Food, Natural Resources and Engineering (DAFNE), University of Foggia, Foggia, Italy  \n2 Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy  \n3 Institute of Sciences of Food Production, National Research Council (CNR) of Italy, C/O CS-DAT, Foggia, Italy  \n4 Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Milan, Italy  \nIntroduction  \nThe large microbial group of lactic acid bacteria (LAB) includes many species that have been traditionally used for food fermentation [1] . Besides, LAB are an interesting source of probiotics, i.e. , live microorganisms that, when ingested, exert a beneficial effect on the host [2] . Thanks to their metabolic versatility, LAB can produce several bioactive compounds, such as bacteriocins, organic acids, bioactive peptides, and B-group vitamins [3 , 4] . Given their beneficial potential, LAB-secreted compounds can be considered postbiotics, i.e. , a term that indicates microbial metabolites and/or inanimate microorganisms with health-promoting properties [5] . The administration of postbiotics could be considered safer compared to probiotics, especially in vulnerable populations such as immunosuppressed, avoiding some potential adverse effects of live microbes [6 , 7] .  \nRiboflavin, also known as vitamin B2, is a B-group, water-soluble vitamin that is essential for the cellular homeostasis. Such molecule is the precursor of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), two cofactors w","cbCail8FMOPD8neA","https://ap.wps.com/l/cbCail8FMOPD8neA","pdf",1292647,17,"English","# Abstract\n## Introduction","[{\"question\":\"How were riboflavin-overproducing Lactiplantibacillus plantarum strains obtained?\",\"answer\":\"Six L. plantarum strains from Mediterranean fruits were exposed to roseoflavin to select spontaneous riboflavin-overproducing phenotypes.\"},{\"question\":\"What main properties were evaluated for the best-performing strain?\",\"answer\":\"The top strain was characterized for antibacterial activity, organic acid production, antibiotic resistance, and survival under digestive stresses using an in vitro gut model.\"},{\"question\":\"What effects did the postbiotic Lp 187_B2 show on the intestinal barrier?\",\"answer\":\"Lp 187_B2 postbiotics significantly increased transepithelial electrical resistance (TEER) in Caco-2 monolayers and inhibited TNF-α release in a Caco-2/THP-1 co-culture model mimicking inflamed bowel.\"}]","Probiotics and Antimicrobial Proteins - Postbiotic Potential of Newly Isolated Riboflavin-Overproducing Lactiplantibacillus plantarum Strains | PDF",1791300953,43]