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Sequencing results indicated shifts in Ascomycota/Basidiomycota ratios and increased relative abundance of genera such as Malassezia, Candida, Saccharomyces, and Aspergillus, suggesting diagnostic potential and the need for validated biomarkers for GI cancer screening.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/predicting-cancer-related-mycobiome-aspects-in-gastrointestinal-cancers-a-systematic-review/329585/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/predicting-cancer-related-mycobiome-aspects-in-gastrointestinal-cancers-a-systematic-review/329585.png","ImageObject",300,407,{"name":92,"@type":93},"Evangeline","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-26","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What was the purpose of this systematic review?","Question",{"text":113,"@type":114},"To evaluate the role of fungal colonization in gastrointestinal tumors and its potential to improve diagnostic efficiency.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"Which sequencing and sample types were included?",{"text":118,"@type":114},"Studies used metagenomic or internal transcribed spacer (ITS) sequencing on tumor tissue, adjacent normal tissue, stool, and blood samples.",{"name":120,"@type":111,"acceptedAnswer":121},"What key microbiome differences were reported between healthy and cancer states?",{"text":122,"@type":114},"The Ascomycota/Basidiomycota (A/B) ratio showed variable directional changes across studies, and relative abundances increased for genera such as Malassezia, Candida, Saccharomyces, and Aspergillus.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},329585,1790032135,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":41,"preview_page_count":4},13056703019662,"https://ap-avatar.wpscdn.com/avatar/be000253a8e92610077?_k=1778726343310543188","OPEN ACCESS  \nEDITED BY  \nPuja Khare,  \nCouncil of Scientific and Industrial Research (CSIR), India  \nREVIEWED BY  \nJayanta Mondal,  \nUniversity of Texas MD Anderson Cancer Center, United States  \nAna Isabel Alvarez-Mercado, Granada Biosanitary Research Institute (ibs.GRANADA), Spain  \n*CORRESPONDENCE  \nZoltan Lohinai  \n [lohinai.zoltan@semmelweis.hu](lohinai.zoltan@semmelweis.hu)[ ](lohinai.zoltan@semmelweis.hu)David Dora  \n [dora.david@.semmelweis.hu](dora.david@.semmelweis.hu)[ ](dora.david@.semmelweis.hu)RECEIVED 29 August 2024 ACCEPTED 13 November 2024 PUBLISHED 29 November 2024  \nCITATION  \nSzklenarik G, Kiraly P, Szegvari G, Dora D and Lohinai Z (2024) Predicting cancer-related mycobiome aspects in gastrointestinal cancers: a systematic review.  \nFront. Med. 11:1488377.  \ndoi: 10.3389/fmed.2024.1488377  \nCOPYRIGHT  \n© 2024 Szklenarik, Kiraly, Szegvari, Dora and Lohinai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.  \nTYPE Systematic Review PUBLISHED 29 November 2024 DOI 10.3389/fmed.2024.1488377  \nPredicting cancer-related mycobiome aspects in gastrointestinal cancers: a systematic review  \nGyörgy Szklenarik 1, Peter Kiraly 1, Gabor Szegvari 1, David Dora 2* and Zoltan Lohinai 1*  \n1Translational Medicine Institute, Semmelweis University, Budapest, Hungary, 2 Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary  \nBackground: Colonization of the human gut and tumor tissue by nonpathogenic fungi has emerged as a potential risk factor associated with cancer epidemics. Therefore, we aimed to conduct a systematic review to assess the role of fungal colonization in gastrointestinal (GI) tumors in increasing diagnostic efficiency.  \nMethods: A PubMed citation search was conducted for publications up to and including March 2023, followed by full-text screening. Results were reported according to Preferred Reporting Items for Systematic Reviews and MetaAnalyses (PRISMA) 2020 guidelines. According to the Patient, Intervention, Comparison, Outcome (PICO) framework, patients diagnosed with early-and advanced-stage GI cancers, GI adenoma patients, and healthy subjects were included with metagenomic (MG) or internal transcribed spacer (ITS) sequencing on tumor tissue, adjacent normal tissue, stool, and blood samples.  \nResults: Fourteen studies were eligible based on the inclusion criteria and methodological quality. Studies were conducted in stool (n = 8) or tissue (n = 7) as the most common specimens to be used for molecular analysis. In the collected data, ITS was used in n = 10 cases and metagenomic analyses in n = 3 cases. Observing the interindividual variability, we found that the Ascomycota/ Basidiomycota (A/B) ratio from healthy to cancer state decreased in n = 2, increased in n = 1 cases, and did not change significantly in n = 2 studies. An increase in the relative abundance of Malassezia was identified in n = 4, of Candida in n = 5, of Saccharomyces in n = 2, and of Aspergillus in n = 2 cases. Intraindividual differences in the A/B ratio were identified in cancer and adjacent tissue (n = 4) and cancer vs. stool (n = 1) studies. Intraindividual variability of the A/B ratio showed an increase in n = 2 and no change in n = 3 studies for cancer tissue.  \nConclusion: In conclusion, the advent of highly sensitive sequencing methods may aid in the identification and the differentiation of cancerous from healthy human fungal colonizations with potential future diagnostic applications. Further studies are needed to establish reliable biomarkers for GI cancer screening.  \nKEYWORDS  \nmycobiome, gastr","cbCait0nfHzqkKoX","https://ap.wps.com/l/cbCait0nfHzqkKoX","pdf",1384827,12,"English","# Background\n# Methods\n# Results\n# Conclusion","[{\"question\":\"What was the purpose of this systematic review?\",\"answer\":\"To evaluate the role of fungal colonization in gastrointestinal tumors and its potential to improve diagnostic efficiency.\"},{\"question\":\"Which sequencing and sample types were included?\",\"answer\":\"Studies used metagenomic or internal transcribed spacer (ITS) sequencing on tumor tissue, adjacent normal tissue, stool, and blood samples.\"},{\"question\":\"What key microbiome differences were reported between healthy and cancer states?\",\"answer\":\"The Ascomycota/Basidiomycota (A/B) ratio showed variable directional changes across studies, and relative abundances increased for genera such as Malassezia, Candida, Saccharomyces, and Aspergillus.\"}]","Predicting cancer-related mycobiome aspects in gastrointestinal cancers - a systematic review | PDF",1789984646]