[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-333236-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-333236-en":131},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","precursor-exhausted-cd8t-cells-in-colorectal-cancer-tissues-associated-with-patients-survival-and-immunotherapy-responsiveness","Precursor exhausted CD8+T cells in colorectal cancer tissues associated with patient’s survival and immunotherapy responsiveness","","Precursor exhausted CD8+T cells (Tpex) arise from persistent antigen exposure and are defined by TCF-1+ PD-1+ CD8+ T-cell features. The study uses multi-color immunohistochemistry to localize Tpex within the colorectal cancer tumor microenvironment and evaluate clinical implications, while integrating multi-omics analyses with single-cell RNA-seq from both MC38 mouse tumors and human colorectal cancer tissues. Spatial transcriptomics further examines Tpex interactions with other immune subsets. Results support mIHC-based Tpex detection and link Tpex assessment in the colorectal cancer microenvironment to patient survival and immunotherapy responsiveness, highlighting transcriptional and functional traits relevant to anti-cancer efficacy.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/precursor-exhausted-cd8t-cells-in-colorectal-cancer-tissues-associated-with-patients-survival-and-immunotherapy-responsiveness/333236/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/precursor-exhausted-cd8t-cells-in-colorectal-cancer-tissues-associated-with-patients-survival-and-immunotherapy-responsiveness/333236.png","ImageObject",300,407,{"name":42,"@type":43},"Finn","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-03","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",13,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What are precursor exhausted (Tpex) CD8+ T cells and how are they defined?","Question",{"text":63,"@type":64},"Tpex cells are a CD8+ T-cell lineage shaped by persistent antigen exposure. They are characterized by TCF-1+ PD-1+ CD8+ marker expression.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How does the study detect and study Tpex cells in colorectal cancer tissues?",{"text":68,"@type":64},"The work uses multi-color immunohistochemistry to determine Tpex localization in the tumor microenvironment. It also applies multi-omics integration with single-cell RNA-seq and uses spatial transcriptome sequencing to analyze interactions with other immune subsets.",{"name":70,"@type":61,"acceptedAnswer":71},"What clinical association does the study report for Tpex cells?",{"text":72,"@type":64},"Assessing Tpex cells within the colorectal cancer tumor microenvironment is associated with patients’ survival. 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Immunol. 15:1362140 .  \ndoi: 10.3389/fimmu.2024.1362140  \nCOPYRIGHT  \n© 2024 Huang, Ge, Fang, Wu, Jiang, Lang, Chen, Xiao, Xu, Liu, Chen, Zheng and Jiang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.  \nPrecursor exhausted  \nCD8 +T cells in colorectal cancer tissues associated with patient’s survival and immunotherapy responsiveness  \nHao Huang 1,2,3, Junwei Ge 1,2,3, Zhang Fang 1,2,3, Shaoxian Wu 1,2,3, Hongwei Jiang 1,2,3, Yanyan Lang 1,2,3, Junjun Chen 1,2,3,  \nWenlu Xiao 1,2,3, Bin Xu 1,2,3, Yingting Liu 1,2,3, Lujun Chen 1,2,3*, Xiao Zheng 1,2,3* and Jingting Jiang 1,2,3*  \n1 Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University, Jiangsu, Changzhou, China, 2Jiangsu Engineering Research Center for Tumor Immunotherapy, The Third Affiliated Hospital of Soochow University, Jiangsu, Changzhou, China, 3 Institute of Cell Therapy, The Third Affiliated Hospital of Soochow University, Jiangsu, Changzhou, China  \nExhausted CD8+T cells represent a distinct cellular lineage that emerges during both chronic infections and cancers. Recent studies have shown that persistent antigen exposure can drive the differentiation of precursor exhausted CD8+T cells, termed Tpex cells, which are characterized as TCF-1+ PD-1+CD8+T cells. Elevated Tpex cell frequencies in the tumor microenvironment (TME) are associated with improved overall survival (OS) in cancer patients and heightened responsiveness to anti-PD-1 therapy. In our present study, we utilized multi-color immuno histochemistry (mIHC) to determine the localization and clinical implications of tumor-infiltrating Tpex cells within the TME of human colorectal cancer (CRC) tissues. We also conducted a multi-omics integrative analysis using single-cell RNA sequencing (scRNA-seq) data derived from both the murine MC38 tumor model and human CRC tissues. This analysis helped delineate the transcriptional and functional attributes of Tpex cells within the CRC TME. Furthermore, we employed spatial transcriptome sequencing data from CRC patients to investigate the interactions between Tpex cells and other immune cell subsets within the TME. In conclusion, our study not only established a method for Tpex cell detection using mIHC technology but also confirmed that assessing Tpex cells within the CRC TME could be indicative of patients ’ survival. We further uncovered the transcriptional and functional characteristics of Tpex cells in the TME and ascertained their pivotal role in the efficacy of immunotherapy against CRC.  \nKEYWORDS  \nprecursor exhausted CD8+T cells, colorectal cancer, multi-color immunohistochemistry, multi-omics, immunotherapy  \nFrontiers in Immunology 01","cbCaiawx8eYTlzYM","https://ap.wps.com/l/cbCaiawx8eYTlzYM","pdf",7758535,"English","# Introduction\n## Immune checkpoint blockade and clinical challenges\n## CD8+T cell function and exhausted states\n## Antigen stimulation, differentiation, and precursor exhaustion\n# Methods and analyses\n## Multi-color immunohistochemistry (mIHC)\n## Multi-omics integration with single-cell RNA sequencing\n## Spatial transcriptome sequencing\n# Results and implications\n## Localization and detection of Tpex in colorectal cancer\n## Association with patient survival\n## Transcriptional and functional characteristics\n## Relevance to immunotherapy efficacy","[{\"question\":\"What are precursor exhausted (Tpex) CD8+ T cells and how are they defined?\",\"answer\":\"Tpex cells are a CD8+ T-cell lineage shaped by persistent antigen exposure. They are characterized by TCF-1+ PD-1+ CD8+ marker expression.\"},{\"question\":\"How does the study detect and study Tpex cells in colorectal cancer tissues?\",\"answer\":\"The work uses multi-color immunohistochemistry to determine Tpex localization in the tumor microenvironment. It also applies multi-omics integration with single-cell RNA-seq and uses spatial transcriptome sequencing to analyze interactions with other immune subsets.\"},{\"question\":\"What clinical association does the study report for Tpex cells?\",\"answer\":\"Assessing Tpex cells within the colorectal cancer tumor microenvironment is associated with patients’ survival. The findings also support a relationship between Tpex biology and immunotherapy responsiveness.\"}]","Precursor exhausted CD8+T cells in colorectal cancer tissues associated with patient’s survival and immunotherapy responsiveness | PDF",1790001597,33]