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Dried plasma spots (DPS) enable self-sampling and simpler storage/transport, but recover only small plasma volumes. This study compares DPS and traditional venous plasma using the Nucleic Acid-Linked Immuno-Sandwich Assay (NULISA) to assess DPS capability for Alzheimer’s biomarker quantification. Results show high NULISA panel reproducibility and measurable concordance between DPS and plasma.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/potential-of-dried-plasma-spot-a-comparative-study-of-plasma-biomarker-quantification-using-nulisa/459379/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/potential-of-dried-plasma-spot-a-comparative-study-of-plasma-biomarker-quantification-using-nulisa/459379.png","ImageObject",300,407,{"name":92,"@type":93},"Miles","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-08","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":19},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why are dried plasma spots (DPS) considered for Alzheimer’s biomarker testing?","Question",{"text":112,"@type":113},"DPS allow self-sampling and easier storage/transport than venipuncture, enabling remote or home-based testing. The tradeoff is that DPS collect only small plasma volumes.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does the study quantify biomarkers from DPS and traditional plasma?",{"text":117,"@type":113},"DPS are prepared by applying blood to Telimmune Duo Plasma Prep Cards, while traditional plasma is collected by centrifugation. Proteins are extracted from DPS using Alamar sample diluent and quantified with NULISAseq CNS Panel on an Argo HT.",{"name":119,"@type":110,"acceptedAnswer":120},"What were the main findings on detectability and agreement between DPS and plasma?",{"text":121,"@type":113},"The NULISAseq CNS panel quantified 127 targets with high reproducibility. In DPS samples, many targets were detected above the limit of detection, and moderate correlations were observed between DPS and traditional plasma for key biomarkers.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},459379,1790755811,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":19,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":14,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":129,"read_time":24,"preview_page_count":4},13056703019404,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","DOI: 10. 1002/alz70856_106865  \nBIOMARKERS  \nPOSTER PRESENTATION  \nBIOMARKERS (NON-NEUROIMAGING)  \nPotential of dried plasma spot: a comparative study of plasma biomarker quantification using NULISA  \nYou-Rim Lee1  Xuemei Zeng 1  Jeremy M. Gu 1  Marissa F Farinas 1   \nJulia K. Kofler 1  Dana L Tudorascu2  C. Elizabeth Shaaban3  Jennifer H Lingler3 Tharick A Pascoal4  William E Klunk3  Victor L. Villemagne5  Sarah B Berman 1 Robert Sweet 1  Beth E. Snitz 1  Milos D. Ikonomovic6  M. Ilyas Kamboh3  Oscar L Lopez 1  Ann D Cohen3  Thomas K Karikari7  \n1 University of Pittsburgh School of Medicine, Pittsburgh, PA, USA  \n2 Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA, USA  \n3 University of Pittsburgh Alzheimer’s Disease Research Center (ADRC), Pittsburgh, PA, USA  \n4 Departments of Psychiatry and Neurology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA  \n5The University of Pittsburgh, Pittsburgh, PA, USA  \n6 UPMC, Pittsburgh, PA, USA  \n7 University of Pittsburgh, Pittsburgh, PA, USA  \nCorrespondence  \nYou-Rim Lee, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. [Email:](Email: you-rim.lee@pitt.edu)[ you-rim.lee@pitt.edu](Email: you-rim.lee@pitt.edu)  \nAbstract  \nBackground: Efforts to diagnose Alzheimer’s disease (AD) through blood tests have identified many promising biomarkers. However, traditional plasma collection via venipuncture requires a medical professional and cold-chain transport, making it unsuitable for remote or home-based testing. Dried plasma spots (DPS) allow selfsampling and easier storage/transport but only collect a small amount of plasma. The Nucleic Acid-Linked Immuno-Sandwich Assay (NULISA) is an innovative platform that quantifies biomarkers from small plasma. This study compares DPS and traditional plasma using NULISA to evaluate DPS’s potential for AD biomarkers.  \nMethod: Venous blood from 85 paricipants atthe University of Pittsburgh Alzheimer’s Disease Research Center, Connectomics in Brain Aging (CoBrA), and INflammation ROles inAging andAlzheimer’s disease Study(INROAADS). DPS samples were created by applying blood to Telimmune Duo Plasma Prep Cards. Traditional plasma samples were collected through centrifugation. Alamar sample diluent was used to extract proteins from DPS. NULISAseq CNS Panel was performed on an Argo HT following the manufacturer’s instructions. The reproducibility of assays was evaluated using a Sample Control run in triplicate across runs. Spearman correlation assessed the concordance between DPS and traditional plasma samples.  \nResult: The NULISAseq CNS panel quantified 127 targets with high reproducibility, showing median intra-and inter-plate coefficients of variation \u003C 10%. In DPS samples, 104 targets (82%) were detected above the limit of detection (LOD) in >50% of samples, compared to 123 (97%) in plasma. Median sample detectability was 82.7% for DPS and 96. 1% for plasma. Among the classical AD biomarkers, NFL, GFAP,  \nThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.  \n© 2025 The Alzheimer’s Association. Alzheimer’s & Dementia published by Wiley Periodicals LLC on behalf of Alzheimer’s Association.  \nAlzheimer’s Dement. 2025;21(Suppl. 2):e106865.  \n[https://doi.org/10.1002/alz70856_106865](https://doi.org/10.1002/alz70856_106865)  \nwi[leyonlinelibrary.com/journal/alz](leyonlinelibrary.com/journal/alz)  \n1of2  \nBIOMARKERS  \nMAPT, p-tau181, p-tau217, and p-tau231 showed high detectability (71% to 99%), while Aβ38, Aβ40, and Aβ42 had low detectability (29% to 35%). A moderate correlation was observed between DPS and plasma samples for these biomarkers, with correlation coefficients ranging from 0.197 to 0.616. Across the whole panel, the median correlation coefficient was 0.369, with NEFH (0.831), CHIT1 (0.829), and IL5 (0.783) showing the highest correlation.  \nConclus","cbCaibSgBVzqljq8","https://ap.wps.com/l/cbCaibSgBVzqljq8","pdf",95396,"English","# Abstract\n# Background\n# Method\n# Result\n# Conclusion","[{\"question\":\"Why are dried plasma spots (DPS) considered for Alzheimer’s biomarker testing?\",\"answer\":\"DPS allow self-sampling and easier storage/transport than venipuncture, enabling remote or home-based testing. The tradeoff is that DPS collect only small plasma volumes.\"},{\"question\":\"How does the study quantify biomarkers from DPS and traditional plasma?\",\"answer\":\"DPS are prepared by applying blood to Telimmune Duo Plasma Prep Cards, while traditional plasma is collected by centrifugation. Proteins are extracted from DPS using Alamar sample diluent and quantified with NULISAseq CNS Panel on an Argo HT.\"},{\"question\":\"What were the main findings on detectability and agreement between DPS and plasma?\",\"answer\":\"The NULISAseq CNS panel quantified 127 targets with high reproducibility. In DPS samples, many targets were detected above the limit of detection, and moderate correlations were observed between DPS and traditional plasma for key biomarkers.\"}]","Potential of dried plasma spot - a comparative study of plasma biomarker quantification using NULISA | PDF"]