[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-365480-105":59,"doc-detail-365480-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","porphyromonas-gingivalis-outer-membrane-vesicles-augments-proliferation-and-metastasis-of-oral-squamous-cell-carcinoma-cells","Porphyromonas gingivalis outer membrane vesicles augments proliferation and metastasis of oral squamous cell carcinoma cells","","Porphyromonas gingivalis (P. gingivalis) is linked to oral squamous cell carcinoma (OSCC), and its outer membrane vesicles (OMVs) are proposed as a key pathogenic factor, yet their relationship with OSCC remains insufficiently studied. This open-access research establishes clinical associations using patient samples and evaluates OMV effects on OSCC cell models in vitro. It further analyzes differential gene expression with RNA-seq and RT-qPCR to clarify mechanisms of OSCC progression.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/porphyromonas-gingivalis-outer-membrane-vesicles-augments-proliferation-and-metastasis-of-oral-squamous-cell-carcinoma-cells/365480/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/porphyromonas-gingivalis-outer-membrane-vesicles-augments-proliferation-and-metastasis-of-oral-squamous-cell-carcinoma-cells/365480.png","ImageObject",300,407,{"name":92,"@type":93},"\tCallum ","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-23",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",15,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the central aim of the study?","Question",{"text":113,"@type":114},"To define the clinical relationship between P. gingivalis and OSCC and to determine how P. gingivalis OMVs affect OSCC cell behavior in vitro, including potential molecular mechanisms.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were P. gingivalis levels assessed in OSCC samples?",{"text":118,"@type":114},"Immunohistochemistry was used to detect P. gingivalis abundance in OSCC and paired paracancer tissues, followed by correlation analyses with patient clinicopathological parameters.",{"name":120,"@type":111,"acceptedAnswer":121},"What effects did P. gingivalis OMVs have on OSCC cells?",{"text":122,"@type":114},"OMVs promoted proliferation and migration of HN6 cells and promoted CAL27 proliferation, with no significant effect on CAL27 migration.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},365480,1790182089,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146},137451211410,"https://ap-avatar.wpscdn.com/avatar/2000bb0a9246f588df?x-image-process=image/resize,m_fixed,w_180,h_180&k=1786362646172706240","Zeng et al. BMCOral Health (2025) 25:701 BMC Oral Health  \n[https://doi.org/10.1186/s12903-025-05937-z](https://doi.org/10.1186/s12903-025-05937-z)  \nRESEARCH Open Access  \nPorphyromonas gingivalis outer membrane vesicles augments proliferation and metastasis of oral squamous cell carcinoma cells  \nYanru Zeng 1,3†, Yiyang Wang 1,3†, Xiaona Shi3, Yuanhao Zhao3, Yue Tang3, Shanshan Liu 1,2 and Xiaofeng Zhu 1,2*  \nAbstract  \nBackground Porphyromonas gingivalis (P. gingivalis) is closely related to Oral squamous cell carcinoma (OSCC), and P. gingivalis outer membrane vesicles (OMVs) is the main pathogenic factor, which is associated with periodontitis, atherosclerosis and other diseases. However, few studies have reported an association between P. gingivalis OMVsand OSCC. The purpose of this study was to establish the clinical relationship between P. gingivalis and OSCC based on clinical samples. Further, the effect of P. gingivalis OMVs on OSCC was observed with cell model in vitro, and the possible molecular mechanism was discussed.  \nMethods Immunohistochemistry was used to detect the abundance of P. gingivalis in OSCC and its paired paracancer tissues, and to analyze the correlation between P. gingivalis and clinicopathological parameters of patients.  \nP. gingivalis OMVs were isolated to observe its effects on the proliferation and migration of OSCC cell lines. RNA-seq was performed and the expression of differential ly expressed genes (DEGs) was detected by real-time quantitative PCR (RT-qPCR) to explore the potential mechnism of P. gingivalis OMVs on OSCC progression.  \nResults The abundance of P. gingivalis in OSCC was higher than that in para-cancerous tissues, and was positively correlated with the degree of tissue differentiation (P = 0 . 028), T stage (P \u003C 0. 001), and clinical stage (P = 0 . 011) . P. gingivalis OMVs promoted the proliferation and migration of HN6 cells, and promoted the proliferation of CAL27 cells, but had no significant effect on its migration. P. gingivalis OMVs treatment attenuated the expressions ofTNFSF15, ZNF292, ATRX, ASPM and KIF20B in CAL27 and HN6 cells.  \nConclusion This study suggests that P. gingivalis may be an indicator of poor prognosis for OSCC. P. gingivalis OMVs may down-regulate the expression of TNFSF15, ZNF292, ATRX, ASPM, KIF20B and participate in the occurrence and development of OSCC.  \nKeywords Squamous cell carcinoma of head and neck, Porphyromonas gingivalis, Extracelular vesicles  \n†Yanru Zeng andYiyang Wang contributed equally to this work.  \n*Correspondence: Xiaofeng Zhu [dentzxf@163.com](dentzxf@163.com)  \n1Department of Oral Maxillo-Facial Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China  \n2Department of Oral Maxillo-Facial Surgery, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, China  \n3Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China  \n© The Author(s) 2025. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation ","cbCaikwxmEBNtB5R","https://ap.wps.com/l/cbCaikwxmEBNtB5R","pdf",5222698,18,"English","# Abstract\n# Background\n# Methods\n# Results\n# Conclusion","[{\"question\":\"What is the central aim of the study?\",\"answer\":\"To define the clinical relationship between P. gingivalis and OSCC and to determine how P. gingivalis OMVs affect OSCC cell behavior in vitro, including potential molecular mechanisms.\"},{\"question\":\"How were P. gingivalis levels assessed in OSCC samples?\",\"answer\":\"Immunohistochemistry was used to detect P. gingivalis abundance in OSCC and paired paracancer tissues, followed by correlation analyses with patient clinicopathological parameters.\"},{\"question\":\"What effects did P. gingivalis OMVs have on OSCC cells?\",\"answer\":\"OMVs promoted proliferation and migration of HN6 cells and promoted CAL27 proliferation, with no significant effect on CAL27 migration.\"}]","Porphyromonas gingivalis outer membrane vesicles augments proliferation and metastasis of oral squamous cell carcinoma cells | PDF",1790161026,45]