[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-380800-105":59,"doc-detail-380800-en":134},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":127,"head_meta":129,"extra_data":131,"updated_unix":133},105,"en","polymorphisms-of-antioxidant-enzymes-sod2-rs4880-and-gpx1-rs1050450-are-associated-with-bladder-cancer-risk-or-its-aggressiveness","Polymorphisms of Antioxidant Enzymes SOD2 (rs4880) and GPX1 (rs1050450) Are Associated with Bladder Cancer Risk or Its Aggressiveness","","Oxidative stress driven by reactive oxygen species (ROS) is central to carcinogenesis, and the urinary tract is continuously exposed to mutagenic agents that generate ROS. This study evaluated whether GPX1 (rs1050450) and SOD2 (rs4880) genetic variants influence urothelial bladder cancer (UBC) risk independently and with smoking, and whether they affect UBC stage and pathological grade. Among 330 UBC patients and 227 controls, SOD2 Val allele carriers showed higher UBC risk than SOD2 Ala16Ala homozygotes (OR=1.55, p=0.03), with a stronger effect in smokers (OR=7.5, p\u003C0.001). GPX1 polymorphism was not associated with UBC risk, but its genotype distribution differed by tumor stage (p\u003C0.049) and pathological grade (p\u003C0.018).",{"@graph":69,"@context":126},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/polymorphisms-of-antioxidant-enzymes-sod2-rs4880-and-gpx1-rs1050450-are-associated-with-bladder-cancer-risk-or-its-aggressiveness/380800/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/polymorphisms-of-antioxidant-enzymes-sod2-rs4880-and-gpx1-rs1050450-are-associated-with-bladder-cancer-risk-or-its-aggressiveness/380800.png","ImageObject",300,407,{"name":92,"@type":93},"RuangKosong","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-31","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118,122],{"name":109,"@type":110,"acceptedAnswer":111},"What was the main goal of this study?","Question",{"text":112,"@type":113},"To assess whether GPX1 (rs1050450) and SOD2 (rs4880) variants are associated with urothelial bladder cancer risk, including interactions with smoking, and whether they relate to disease stage and pathological grade.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were SOD2 and GPX1 polymorphisms analyzed?",{"text":117,"@type":113},"GPX1 (rs1050450) was analyzed using PCR-RFLP, while SOD2 (rs4880) was analyzed using the q-PCR method.",{"name":119,"@type":110,"acceptedAnswer":120},"What relationship did the study find between SOD2 rs4880 and UBC risk?",{"text":121,"@type":113},"Carriers of at least one SOD2 Val allele had significantly increased UBC risk compared with SOD2 Ala16Ala homozygotes, and the risk was even more pronounced among smokers.",{"name":123,"@type":110,"acceptedAnswer":124},"Did GPX1 rs1050450 show an association with UBC risk and aggressiveness?",{"text":125,"@type":113},"No association was found with overall UBC risk, but GPX1 genotype distribution differed significantly by tumor stage and pathological grade, indicating a link to disease aggressiveness.","https://schema.org",{"og:url":83,"og:type":128,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":130,"canonical":83},"index,follow",{"doc_id":132,"site_id":62},380800,1790691086,{"code":4,"msg":5,"data":135},{"doc_id":132,"user_id":136,"nickname":92,"user_avatar":137,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":138,"file_id":139,"file_url":140,"file_type":141,"file_size":142,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":143,"language":144,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":145,"faqs":146,"seo_title":147,"seo_description":67,"update_tm":148,"read_time":41},962090883219,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","medicina  \nArticle  \nPolymorphisms of Antioxidant Enzymes SOD2 (rs4880) and GPX1 (rs1050450) Are Associated with Bladder Cancer Risk or Its Aggressiveness  \nPredrag Nikic 1,2, Dejan Dragicevic 1,2, Djurdja Jerotic 2,3, Slaviša Savic 2,4, Tatjana Djukic 2,3, Branko Stankovic 1, Luka Kovacevic 1, Tatjana Simic 2,3,5 and Marija Matic 2,3, *  \nCitation: Nikic, P.; Dragicevic, D.; Jerotic, D.; Savic, S.; Djukic, T.;  \nStankovic, B.; Kovacevic, L.; Simic, T.; Matic, M. Polymorphisms of Antioxidant Enzymes SOD2 (rs4880) and GPX1 (rs1050450) Are Associated with Bladder Cancer Risk or Its Aggressiveness. Medicina 2023, 59, 131. [https://doi.org/10.3390/](https://doi.org/10.3390/)[ ](https://doi.org/10.3390/)medicina59010131  \nAcademic Editor: Joo Yong Lee  \nReceived: 5 November 2022  \nRevised: 19 December 2022  \nAccepted: 29 December 2022  \nPublished: 9 January 2023  \nCopyright: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Clinic of Urology, Clinical Center of Serbia, 11000 Belgrade, Serbia  \n2 Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia  \n3 Institute of Medical and Clinical Biochemistry, 11000 Belgrade, Serbia  \n4 Department of Urology, KBC Dr. Dragiša Mišovic, 11000 Belgrade, Serbia  \n5 Serbian Academy of Sciences and Arts, 11000 Belgrade, Serbia  \n* [Correspondence: marija.matic@med.bg.ac.rs](Correspondence: marija.matic@med.bg.ac.rs); Tel.: +381-63-319-634  \nAbstract: Background and Objectives: Oxidative stress induced by increased reactive oxygen species (ROS) production plays an important role in carcinogenesis. The entire urinary tract is continuously exposed to numerous potentially mutagenic environmental agents which generate ROS during their biotransformation. In first line defense against free radicals, antioxidant enzymes superoxide dismutase (SOD2) and glutathione peroxidase (GPX1) both have essential roles. Altered enzyme activity and decreased ability of neutralizing free oxygen radicals as a consequence of genetic polymorphisms in genes encoding these two enzymes are well described so far. This study aimed to investigate the association of GPX1 (rs1050450) and SOD2 (rs4880) genetic variants with theurothelial bladder cancer (UBC) risk independently and in combination with smoking. Furthermore, we aimed to determine whether the UBC stage and pathological grade were influenced by GPX1 and SOD2 polymorphisms. Material and Methods: The study population included 330 patients with UBC (mean age 65 􀀆 10.3 years) and 227 respective controls (mean age 63.4 􀀆 7.9 years) . Single nucleotide polymorphism (SNP) of GPX1 (rs1050450) was analyzed using the PCR-RFLP, while SOD2 (rs4880) SNP was analyzed using the q-PCR method. Results: Our results showed that UBC risk was significantly increased among carriers of at least one variant SOD2 Val allele compared to the SOD2 Ala16Ala homozygotes (OR = 1.55, p = 0.03) . Moreover, this risk was even more pronounced in smokers with at least one variant SOD2 Val allele, since they have even 7.5 fold higher UBC risk (OR = 7.5, p \u003C 0.001) . Considering GPX1 polymorphism, we have not found an association with UBC risk. However, GPX1 genotypes distribution differed significantly according to the tumor stage (p \u003C 0.049) and pathohistological grade (p \u003C 0.018) . Conclusion: We found that SOD2 genetic polymorphism is associated with the risk of UBC development independently and in combination with cigarette smoking. Furthermore, we showed that GPX1 genetic polymorphism is associated with the aggressiveness of the disease.  \nKeywords: urothelial bladder cancer; risk; genetic polymorphisms; SNP; GPX1; SOD2  \n1. Introduction  \nBladder cancer is the second most common genitourinary mali","cbCaicjz8D3sEw2Q","https://ap.wps.com/l/cbCaicjz8D3sEw2Q","pdf",364706,12,"English","# Introduction\n## Oxidative stress and ROS in carcinogenesis\n## Bladder cancer background and risk factors\n# Materials and Methods\n## Study population and SNP genotyping\n# Results\n## Association with UBC risk and smoking\n## Association with tumor stage and pathological grade\n# Conclusion","[{\"question\":\"What was the main goal of this study?\",\"answer\":\"To assess whether GPX1 (rs1050450) and SOD2 (rs4880) variants are associated with urothelial bladder cancer risk, including interactions with smoking, and whether they relate to disease stage and pathological grade.\"},{\"question\":\"How were SOD2 and GPX1 polymorphisms analyzed?\",\"answer\":\"GPX1 (rs1050450) was analyzed using PCR-RFLP, while SOD2 (rs4880) was analyzed using the q-PCR method.\"},{\"question\":\"What relationship did the study find between SOD2 rs4880 and UBC risk?\",\"answer\":\"Carriers of at least one SOD2 Val allele had significantly increased UBC risk compared with SOD2 Ala16Ala homozygotes, and the risk was even more pronounced among smokers.\"},{\"question\":\"Did GPX1 rs1050450 show an association with UBC risk and aggressiveness?\",\"answer\":\"No association was found with overall UBC risk, but GPX1 genotype distribution differed significantly by tumor stage and pathological grade, indicating a link to disease aggressiveness.\"}]","Polymorphisms of Antioxidant Enzymes SOD2 (rs4880) and GPX1 (rs1050450) Are Associated with Bladder Cancer Risk or Its Aggressiveness | PDF",1790242180]