[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-434167-105":59,"doc-detail-434167-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","polydatin-protects-against-the-formation-of-ppe-induced-abdominal-aortic-aneurysms-in-mice-by-activating-nuclear-factor-erythroid-2-related-factor-2","Polydatin Protects Against the Formation of PPE-Induced Abdominal Aortic Aneurysms in Mice by Activating Nuclear Factor Erythroid 2-Related Factor 2","","Sudden death after abdominal aortic aneurysm (AAA) rupture is a highly fatal cardiovascular event, and there is an urgent need for therapies that slow AAA progression and rupture. Although polydatin (PLD) is known for strong anti-inflammatory effects, its role in AAA remains unclear. In a porcine pancreatic elastase (PPE)-infusion mouse model, PLD’s impact on NRF2 signaling was assessed using Western blot, immunohistochemistry, immunofluorescence, flow cytometry, and RT-qPCR. PLD activated NRF2, reduced AAA formation by limiting smooth muscle cell phenotypic transformation and apoptosis, and suppressed macrophage-driven inflammatory responses.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/polydatin-protects-against-the-formation-of-ppe-induced-abdominal-aortic-aneurysms-in-mice-by-activating-nuclear-factor-erythroid-2-related-factor-2/434167/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/polydatin-protects-against-the-formation-of-ppe-induced-abdominal-aortic-aneurysms-in-mice-by-activating-nuclear-factor-erythroid-2-related-factor-2/434167.png","ImageObject",300,407,{"name":92,"@type":93},"Rhys","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-07","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What problem does the study address regarding abdominal aortic aneurysm (AAA)?","Question",{"text":112,"@type":113},"AAA rupture can lead to sudden death and has a high mortality rate. The study aims to find therapeutic strategies that can slow AAA progression and reduce rupture risk.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was the AAA model induced and how was PLD evaluated?",{"text":117,"@type":113},"The study used a porcine pancreatic elastase (PPE) infusion–induced AAA mouse model. PLD’s effects on NRF2 signaling were measured using Western blot, immunohistochemistry, immunofluorescence, flow cytometry, and RT-qPCR.",{"name":119,"@type":110,"acceptedAnswer":120},"What key protective mechanism did PLD show in the findings?",{"text":121,"@type":113},"PLD robustly activated the NRF2 pathway, attenuating AAA formation by reducing smooth muscle cell phenotypic transformation and apoptosis, and suppressing macrophage-mediated inflammatory responses.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},434167,1790763212,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":41,"preview_page_count":4},687207024643,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","Drug Development Research   \nRESEARCH ARTICLE   \nPolydatin Protects Against the Formation of PPE‐Induced Abdominal Aortic Aneurysms in Mice by Activating Nuclear Factor Erythroid 2‐Related Factor 2  \nZikang Liu1 | Jinyi Zhu2 | Qian Zhou1 | Haiyun Qian1 | Fenghe Cui1   \n1Department of Cardiothoracic Surgery, Jingzhou Central Hospital Affiliated to Yangtze University, Jingzhou City, Hubei Province, China | 2Department of Radiology, Jingzhou Central Hospital Affiliated to Yangtze University, Jingzhou City, Hubei Province, China  \nCorrespondence: Fenghe Cui ([15727210074@163.com](15727210074@163.com))  \nReceived: 23 July 2025 | Revised: 24 October 2025 | Accepted: 27 December 2025  \nFunding: Jingzhou Hospital Affiliated to Yangtze University  \nKeywords: abdominal aortic aneurysm | apoptosis | nuclear factor erythroid 2‐related factor 2 | polydatin  \nABSTRACT  \nSudden death resulting from abdominal aortic aneurysm (AAA) rupture is a highly fatal cardiovascular condition. There remains an urgent need to identify innovative therapeutic strategies capable of decelerating AAA progression and rupture. Although polydatin (PLD) has been consistently shown to exert potent anti‐inflammatory effects in diverse pathological contexts, its regulatory role in AAA has not yet been investigated. Using a porcine pancreatic elastase (PPE) infusion–induced AAA mouse model, we comprehensively evaluated PLD's influence on NRF2 signaling by Western blot (WB) analysis, immunohistochemistry (IHC), immunofluorescence (IF), flow cytometry (FC), and real‐time quantitative PCR (RT‐qPCR) tests. Our findings demonstrate that PLD robustly activates NRF2 pathway, thereby attenuating AAA formation by curbing smooth muscle cell (SMCs) phenotypic transformation and apoptosis, as well as suppressing macrophage‐mediated inflammatory responses.  \n1 | Introduction  \nAn aortic aneurysm is defined as a localized dilatation of the aorta exceeding 50% of its normal diameter (Sakalihasan et al. 2005) . The pathological features of abdominal aortic aneurysm (AAA) include the depletion of smooth muscle cells (SMCs), degradation of extracellular matrix (ECM), and infiltration of inflammatory cells (Sakalihasan et al. 2005) . These factors lead to progressive elevations of arterial wall instability, loss of integrity, rupture, and death. The mortality rate can be as high as 60% once an AAA rupture occurs (Arnaoutakis and Upchurch 2019) . Unfortunately, there is no effective treatment other than surgery. Identifying effective therapeutic agents is a critical step toward preventing AAA progression.  \nActivated macrophages, as a crucial component among the infiltrating inflammatory cells, play an essential role in the initiation and progression of AAAs (Raffort et al. 2017) . These  \ninfiltrated macrophages within AAAs, through their production of matrix metalloproteinases (MMPs), contribute to the degradation of the ECM. Simultaneously, the substantial release of proinflammatory cytokines elevates the levels of reactive oxygen species (ROS) in SMCs, exacerbating the phenotypic transformation and apoptosis of SMCs (Lim et al. 2010) . Thus, alleviating proinflammatory macrophage activation and reducing oxidative stress levels in SMCs represents a promising approach for the treatment of AAAs.  \nNRF2 serves as a pivotal guardian of redox homeostasis, playing a crucial role in defending against oxidative stress (Yamamoto et al. 2018) . The activation of NRF2 leads to the upregulation of genes associated with anti‐oxidative stress mechanisms, including NAD(P)H quinone oxidoreductase (NQO1) and Glutathione Peroxidase 4 (GPX4) (Zhang et al. 2021) . Notably, acrosstalk between NRF2 and P65 has been identified in previous  \nThis is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptation","cbCaifBBjQbLQezq","https://ap.wps.com/l/cbCaifBBjQbLQezq","pdf",7096844,12,"English","# Introduction\n# Methods and Materials\n## Animals\n## Cells and Treatment","[{\"question\":\"What problem does the study address regarding abdominal aortic aneurysm (AAA)?\",\"answer\":\"AAA rupture can lead to sudden death and has a high mortality rate. The study aims to find therapeutic strategies that can slow AAA progression and reduce rupture risk.\"},{\"question\":\"How was the AAA model induced and how was PLD evaluated?\",\"answer\":\"The study used a porcine pancreatic elastase (PPE) infusion–induced AAA mouse model. PLD’s effects on NRF2 signaling were measured using Western blot, immunohistochemistry, immunofluorescence, flow cytometry, and RT-qPCR.\"},{\"question\":\"What key protective mechanism did PLD show in the findings?\",\"answer\":\"PLD robustly activated the NRF2 pathway, attenuating AAA formation by reducing smooth muscle cell phenotypic transformation and apoptosis, and suppressing macrophage-mediated inflammatory responses.\"}]","Polydatin Protects Against the Formation of PPE-Induced Abdominal Aortic Aneurysms in Mice by Activating Nuclear Factor Erythroid 2-Related Factor 2 | PDF",1790667361]