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This retrospective study evaluates whether plasma methylation of SHOX2, SEPTIN9, EPO, and RNF180 can support clinical diagnosis and disease monitoring. Plasma from 210 patients was analyzed using TaqMan PCR, and diagnostic performance was assessed by receiver operating characteristics. Combined gene methylation showed relevant sensitivity and specificity for detecting adenocarcinoma, squamous cell carcinoma, and overall esophageal cancer.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/plasma-dna-methylation-detection-for-early-screening-diagnosis-and-monitoring-of-esophageal-adenocarcinoma-and-squamous-cell-carcinoma/337037/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/plasma-dna-methylation-detection-for-early-screening-diagnosis-and-monitoring-of-esophageal-adenocarcinoma-and-squamous-cell-carcinoma/337037.png","ImageObject",300,407,{"name":92,"@type":93},"Seraphina","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the goal of this study on plasma DNA methylation?","Question",{"text":112,"@type":113},"To investigate the clinical utility of plasma SHOX2, SEPTIN9, EPO, and RNF180 methylation for early screening, diagnosis, and monitoring of esophageal cancer.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were plasma methylation markers measured?",{"text":117,"@type":113},"Plasma samples from 210 patients were tested for SHOX2, SEPTIN9, RNF180, and EPO methylation using TaqMan polymerase chain reaction.",{"name":119,"@type":110,"acceptedAnswer":120},"What diagnostic performance did the combined methylation assessment show?",{"text":121,"@type":113},"Combined assessment demonstrated sensitivity and specificity for detecting adenocarcinoma, squamous cell carcinoma, and esophageal cancer, with area under the curve values of 0.737, 0.824, and 0.864, respectively.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},337037,1790057821,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":41},962075114101,"https://ap-avatar.wpscdn.com/avatar/e000253a75eb197efd?x-image-process=image/resize,m_fixed,w_180,h_180&k=1780044092746381165","| W | J | G | World Journal of\u003Cbr>Gastroenterology |\n| --- | --- | --- | --- |\n\nSubmit a Manuscript: [https://www.f6publishing.com](https://www.f6publishing.com) World J Gastroenterol 2024 November 21; 30(43): 4609-4619  \nDOI: 10.3748/wjg.v30.i43.4609 ISSN 1007-9327 (print) ISSN 2219-2840 (online)  \nORIGINAL ARTICLE  \nRetrospective Study  \nPlasma DNA methylation detection for early screening, diagnosis, and monitoring of esophageal adenocarcinoma and squamous cell carcinoma  \nXu-Ji Liu, Guo-Liang Pi, Sheng Wang, Jin-Dan Kai, Hui-Fang Yu, Hong-Wei Shi, Jing Yu, Hui Zeng  \nSpecialty type: Gastroenterology and hepatology  \nProvenance and peer review:  \nUnsolicited article; Externally peer reviewed.  \nPeer-review model: Single blind  \nPeer-review report’s classification Scientific Quality: Grade C, Grade C  \nNovelty: Grade B, Grade C  \nCreativity or Innovation: Grade B, Grade B  \nScientific Significance: Grade B, Grade B  \nP-Reviewer: Brown J; Chen Q  \nReceived: June 10, 2024  \nRevised: September 6, 2024  \nAccepted: September 13, 2024  \nPublished online: November 21, 2024  \nProcessing time: 142 Days and 17.9 Hours  \nXu-Ji Liu, Hui Zeng, Department of Radiotherapy and Oncology, Wuhan Sixth Hospital and Affiliated Hospital of Jianghan University, Wuhan 430015, Hubei Province, China  \nXu-Ji Liu, Department of Radiotherapy and Oncology, Jianghan University, School of Medicine, Wuhan 430015, Hubei Province, China  \nGuo-Liang Pi, Hong-Wei Shi, Department of Radiation Oncology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430079, Hubei Province, China  \nSheng Wang, Jin-Dan Kai, Hui-Fang Yu, Department of Thoracic Surgery, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430079, Hubei Province, China  \nJing Yu, Department of Laboratory Medicine, Wuhan Hospital of Traditional Chinese and Western Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China  \nCo-first authors: Xu-Ji Liu and Guo-Liang Pi.  \nCo-corresponding authors: Jing Yu and Hui Zeng.  \nCorresponding author: Jing Yu, PhD, Doctor, Department of Laboratory Medicine, Wuhan Hospital of Traditional Chinese and Western Medicine, Tongji Medical College, Huazhong University of Science and Technology, No. 215 Zhongshan Road, Wuhan 430022, Hubei Province, [China.](China. yujings9774@sina.com.cn)[ yujings9774@sina.com.cn](China. yujings9774@sina.com.cn)  \nAbstract  \nBACKGROUND  \nThe early diagnosis rate of esophageal cancer (EC), one of the most prevalent digestive tract cancers worldwide, remains low.  \nAIM  \nTo investigate the utility of plasma SHOX2, SEPTIN9, EPO, and RNF180 methylation in the clinical diagnosis and monitoring of EC.  \n WJG  [https://www.wjgnet.com](https://www.wjgnet.com) 4609 November 21, 2024  Volume 30  Issue 43   \nLiu XJ et al. Plasma DNA methylation for EC diagnosis  \nMETHODS  \nPlasma samples were collected from 210 patients at Hubei Cancer Hospital, and TaqMan polymerase chain reaction was employed to detect plasma SHOX2, SEPTIN9, RNF180, and EPO methylation. The area under the curve was used to estimate their diagnostic value for EC. Cox and logistic regression analyses were used to estimate the independent screening risk factors for patients with EC.  \nRESULTS  \nThe sensitivity and specificity of combined assessment of plasma SHOX2, SEPTIN9, RNF180, and EPO methylation for adenocarcinoma, squamous cell carcinoma (SCC), and EC detection were 66.67% and 86.27%, 77.40% and 85.29%, and 76.19% and 86.27%, respectively; the area under the curve values for diagnosing adenocarcinoma, SCC, and EC were 0.737 [95% confidence interval (CI): 0.584–0.89], 0.824 (95%CI: 0.775–0.891), and 0.864 (95%CI: 0.809–0.92), respectively.  \nCONCLUSION  \nAccording to our findings, plasma SHOX2, SEPTIN9, RNF180, and EPO methylation exhibits appreciated sensitivity for diagnosing EC. The precise measurement of plasma SHOX2, ","cbCaigcMfGb6yTM9","https://ap.wps.com/l/cbCaigcMfGb6yTM9","pdf",3144596,12,"English","# Abstract\n## Background\n## Aim\n## Methods\n## Results\n## Conclusion\n# Core Tip\n# Citation","[{\"question\":\"What is the goal of this study on plasma DNA methylation?\",\"answer\":\"To investigate the clinical utility of plasma SHOX2, SEPTIN9, EPO, and RNF180 methylation for early screening, diagnosis, and monitoring of esophageal cancer.\"},{\"question\":\"How were plasma methylation markers measured?\",\"answer\":\"Plasma samples from 210 patients were tested for SHOX2, SEPTIN9, RNF180, and EPO methylation using TaqMan polymerase chain reaction.\"},{\"question\":\"What diagnostic performance did the combined methylation assessment show?\",\"answer\":\"Combined assessment demonstrated sensitivity and specificity for detecting adenocarcinoma, squamous cell carcinoma, and esophageal cancer, with area under the curve values of 0.737, 0.824, and 0.864, respectively.\"}]","Plasma DNA methylation detection for early screening, diagnosis, and monitoring of esophageal adenocarcinoma and squamous cell carcinoma | PDF",1790017475]