[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-496831-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-496831-en":131},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","phytofuse-renew-il-6-elisa-analysis-version-6","Phytofuse Renew IL-6 ELISA Analysis - Version 6","","Interleukin-6 (IL-6) ELISA assessed how Phytofuse Renew® affects IL-6 production in vitro in human dermal fibroblasts. The assay used IL-6–specific monoclonal antibodies with HRP-labeled detection and TMB-based colorimetric readout at 450 nm. Fibroblasts were stimulated with LPS (1 µg/mL) and treated with Phytofuse Renew® at 1%, 0.1%, and 0.01% for 24 hours. Results showed valid controls and a dose-dependent reduction in IL-6 levels versus the positive control, with percent decrease calculated accordingly.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/phytofuse-renew-il-6-elisa-analysis-version-6/496831/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/phytofuse-renew-il-6-elisa-analysis-version-6/496831.png","ImageObject",300,407,{"name":42,"@type":43},"Riley West","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-29","2026-10-02",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",14,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What biological role of IL-6 is discussed in the study’s introduction?","Question",{"text":63,"@type":64},"IL-6 is described as a proinflammatory cytokine involved in inflammation, immunology, bone metabolism, reproduction, arthritis, neoplasia, and aging. It signals via the NF-κB pathway to drive inflammatory mediator transcription, which relates to skin extracellular matrix breakdown.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How was the IL-6 ELISA performed in this analysis?",{"text":68,"@type":64},"A colorimetric ELISA was used with IL-6–specific monoclonal antibodies coated on a microtiter plate. Bound IL-6 was detected with an HRP-labeled IL-6 antibody, followed by TMB chromagen development and optical density reading at 450 nm using a microplate reader.",{"name":70,"@type":61,"acceptedAnswer":71},"What treatment conditions and concentrations of Phytofuse Renew® were tested?",{"text":72,"@type":64},"Normal human dermal fibroblasts were stimulated with LPS (1 µg/mL) and treated with Phytofuse Renew® at 1%, 0.1%, and 0.01% in complete DMEM for 24 hours. Positive controls contained LPS in complete media, and a negative control used complete DMEM.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},496831,1791359982,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,120,123,127],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},8,"Research & Report",30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":124,"doc_module":4,"doc_module_name":25,"category_name":125,"show_sort_weight":124,"slug":126},10,"Lifestyle","lifestyle",{"id":128,"doc_module":4,"doc_module_name":25,"category_name":129,"show_sort_weight":97,"slug":130},19,"General","general",{"code":4,"msg":82,"data":132},{"doc_id":79,"user_id":133,"nickname":42,"user_avatar":134,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":33,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":124,"preview_page_count":4},1099523885074,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","Tradename: Phytofuse Renew®  \nCode: 16586  \nCAS \\#: 90106-73-3  \nTest Request Form \\#: 3979  \nLot\\#: NC180213-C  \nSponsor: Active Concepts, LLC; 107 Technology Drive Lincolnton, NC 28092  \nStudy Director: Erica Segura  \nPrinciple Investigator: Maureen Danaher  \nTest Performed:  \nInterleukin (IL)-6 Enzyme-Linked Immunosorbent Assay (ELISA)  \nIntroduction  \nInterleukin-6 is a proinflammatory cytokine known to play an active role in inflammation, immunology, bone metabolism, reproduction, arthritis, neoplasia, and aging. IL-6 signals through the nuclear factor-kappa B (NF-􀁎B) pathway that results in the transcription of inflammatory mediators, including matrix metalloproteinase-1 (MMP-1) . MMP’s are responsible for breaking down the extracellular matrix and collagen in the skin leading to wrinkles, fine lines , and loss of skin elasticity. Reducing the level of IL-6 and other inflammatory mediators is believed to slow down degradation of the skin matrix and, possibly, stimulate its replenishment.  \nInterleukin-6 ELISA was conducted to assess the changes in IL-6 levels in Phytofuse Renew®-treated in vitro cultured human dermal fibroblasts.  \nAssay Principle  \nThis ELISA utilizes a colorimetric reaction employing antibodies with antigen specificity to human IL-6 . Monoclonal antibodies specific for IL-6 epitopes are coated on a microtiter plate. In positive samples, IL-6 will bind to these antibodies and are tagged a second time with another IL-6-specific antibody labeled with horseradish peroxidase (HRP) . The addition of the chromagen solution, containing 3,3’,5,5’-tetramethylbenzidine, provides the colorimetric reaction with HRP that is quantitated through optical density (OD) readings on a microplate spectrometer. The standard curve provides a reference from the OD readings for the amount of collagen in each sample.  \nMaterials  \nA. Kit: IL-6 ELISA Kit (Biosource; KAC1261)  \nB. Incubation Conditions: 37°C at 5% CO2 and 95% relative humidity (RH)  \nC. Equipment: Forma humidified incubator; ESCO biosafety laminar flow hood; Microplate Reader; Pipettes  \nD. Cell Line: Normal Human Dermal Fibroblasts (NHDF) (Lonza; CC-2511)  \nE. Media/Buffers: Dulbecco's Modified Eagle Medium (DMEM); PenicillinStreptomycin (50U-50mg/mL); Fetal Bovine Serum (FBS); Phosphate Buffered Saline (PBS); Amphotericin (45pg/mL)  \nF. Culture Plate: Falcon flat bottom 12-well tissue culture treated plates  \nG. Reagents: Lipopolysaccharide (LPS) (1µg/mL)  \nH. Other: Sterile disposable pipette tips; wash bottles  \nMethods  \nHuman dermal fibroblasts were seeded into 12-well tissue culture plates and allowed to grow to confluency in complete DMEM. 1%, 0. 1%, 0.01% concentrations of Phytofuse Renew® were added to complete DMEM containing 1µg/mL LPS and incubated with fibroblasts for 24 hours. Complete media containing 1µg/mL LPS was used as the positive controls and complete DMEM was used a negative control.  \nStandards were prepared in concentrations ranging from 2476pg/mL to 0pg/mL. 50µL of Solution B was added to wells for standards and assay controls and 50µL of Solution A was added to experiment wells. 100µL of standards, controls, and samples were added to appropriate wells. After a one hour incubation at room temperature and washing , 50µL Solution A and 100µL anti-IL-6 conjugate was added to all wells. Following a one hour incubation and washing , 100 µL chromagen solution was added for the colorimetric reaction. One-hundred µL stop solution was added to stop the reaction after 15 minutes. The optical density was read at 450nm on the Synergy HT Microplate Reader.  \nA standard curve was created by reducing the data and generating a linear curve fit. The IL-6 concentration of Phytofuse Renew® treated-fibroblasts was determined by extrapolation from the standard curve and expressed in pg/mL.  \nThis information is presented in good faith but is not warranted as to accuracy of results. Also, freedom from patent infringement is not implied.  \nThis information is ","cbCainjHrtqwPxdl","https://ap.wps.com/l/cbCainjHrtqwPxdl","pdf",417383,"English","# Introduction\n## Assay Principle\n## Materials\n## Methods\n## Results\n## Discussion","[{\"question\":\"What biological role of IL-6 is discussed in the study’s introduction?\",\"answer\":\"IL-6 is described as a proinflammatory cytokine involved in inflammation, immunology, bone metabolism, reproduction, arthritis, neoplasia, and aging. It signals via the NF-κB pathway to drive inflammatory mediator transcription, which relates to skin extracellular matrix breakdown.\"},{\"question\":\"How was the IL-6 ELISA performed in this analysis?\",\"answer\":\"A colorimetric ELISA was used with IL-6–specific monoclonal antibodies coated on a microtiter plate. Bound IL-6 was detected with an HRP-labeled IL-6 antibody, followed by TMB chromagen development and optical density reading at 450 nm using a microplate reader.\"},{\"question\":\"What treatment conditions and concentrations of Phytofuse Renew® were tested?\",\"answer\":\"Normal human dermal fibroblasts were stimulated with LPS (1 µg/mL) and treated with Phytofuse Renew® at 1%, 0.1%, and 0.01% in complete DMEM for 24 hours. Positive controls contained LPS in complete media, and a negative control used complete DMEM.\"}]","Phytofuse Renew IL-6 ELISA Analysis - Version 6 | PDF",1790941429]