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It explains why the recommended order of draw prevents additive carryover and tube-to-tube specimen contamination, reducing erroneous results that can affect diagnosis and patient management. Learning covers blood components and specimen types, tube additives, special collection needs such as discard tubes and blood cultures, and how tube selection, fill volume, and mixing influence integrity and downstream coagulation and chemistry values.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/phlebotomy-order-of-draw-course-description/433318/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/phlebotomy-order-of-draw-course-description/433318.png","ImageObject",300,407,{"name":92,"@type":93},"Aditya","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why is the order of draw important in phlebotomy?","Question",{"text":112,"@type":113},"Collecting tubes in the wrong order can allow additives to carry over into subsequent tubes, causing specimen contamination and erroneous laboratory values. This can lead to misinterpretation, delayed diagnosis, or inappropriate patient management.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How do whole blood, plasma, and serum differ for laboratory testing?",{"text":117,"@type":113},"Whole blood contains plasma and formed elements and is collected in anticoagulant tubes to prevent clotting. Plasma is separated after clotting is prevented and is used for coagulation and many chemistry tests, while serum is collected by allowing blood to clot in an anticoagulant-free tube before centrifugation.",{"name":119,"@type":110,"acceptedAnswer":120},"What factors can alter specimen integrity and test results?",{"text":121,"@type":113},"Improper tube selection, incorrect fill volume, and inadequate mixing can affect specimen integrity. Incorrect order of draw can further impact laboratory results, including coagulation and chemistry values.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},433318,1791019207,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":41,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},962085564549,"https://ap-avatar.wpscdn.com/davatar_085a072bc5b1113ac321206ff7593b45","Phlebotomy Order of Draw  \nCOURSE DESCRIPTION  \nAccurate laboratory results begin with proper specimen collection. Following the correct order of draw is essential to prevent additive carryover and specimen contamination, which can lead to erroneous test results and impact patient care.  \nThis course explains the rationale behind the recommended order of draw and reviews the relationship between blood components, specimen types, and tube additives. Participants will also examine key collection practices that affect specimen integrity and learn how improper technique can alter laboratory results. Practical examples and case studies are included to reinforce correct collection procedures and support safe, accurate phlebotomy practice.  \n*Valid for P.A.C.E.® credit through  07/31/2028  *  \n* ASCLS P.A. C. E.® is an approved continuing education agency by the California Department of Health Laboratory Field Services, Accrediting Agency \\#0001 .  \n*NCCT is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A. C. E. ® Program, provider \\#122 .  \nRev 15  \nApril 2026  \nCOPYRIGHT  \n© 2026 National Center for Competency Testing  \nReproduction or translation of any part of this work beyond that permitted by Sections 107 or 108 of the 1976 United States Copyright Act without the permission of the copyright owner is unlawful. No part of this work may be reproduced or used in any form or by any means-graphic, electronic, or mechanical, including photocopying, recording, taping, or information storage and retrieval systems without written permission of the publisher.  \nCOURSE TITLE: Phlebotomy Order of Draw  \nAuthor: Lucia Johnson, M.A., EdD, MT(ASCP)SBB Revised by: Tami Maffitt, MBA, MLS(ASCP)CM  \nNational Center for Competency Testing  \nNumber of Clock Hours Credit: 1.0  \nCourse \\# 1221026  \nLevel of Instruction: Intermediate  \nP.A.C.E.® Approved:  X  Yes   No  \nConflict of Interest Statement: The authors and editors have no financial or personal conflicts of interest to disclose related to this course’s content.  \nIntended Audience: Phlebotomy technicians, medical assistants, and patient care techs who perform phlebotomy. This course is also appropriate for any healthcare worker in possession of a valid NCCT credential.  \nObjectives: Upon completion of this continuing education course, the professional should be able to:  \n1. Identify the recommended order of draw for venipuncture and capillary blood collection.  \n2. Identify common evacuated tube types, their additives, and associated specimen types (e.g., serum, plasma, whole blood) .  \n3. Recognize situations requiring special collection procedures, including the use of discard tubes and blood culture collection.  \n4. Identify the organization responsible for establishing order of draw guidelines.  \n5. Explain the rationale for the order of draw in preventing additive carryover and specimen contamination.  \n6. Differentiate between plasma, serum, and whole blood in relation to laboratory testing requirements.  \n7. Explain the impact of improper tube selection, fill volume, and mixing on specimen integrity and test results.  \n8. Interpret how incorrect order of draw can affect laboratory test results, including coagulation and chemistry values.  \n9. Select the appropriate tube type and sequence of collection based on ordered laboratory tests and specimen requirements.  \nDisclaimer  \nThe writers for NCCT continuing education courses attempt to provide factual information based on literature review and current professional practice. However, NCCT does not guarantee that the information contained in the continuing education courses is f ree from all errors and omissions.  \n\n|  | INTRODUCTION\u003Cbr>Blood collection is often viewed as a routine task, but improper technique-particularly collecting tubes in the wrong order-can significantly alter laboratory test results. Additives from one tube may carry over into subsequent tubes, causing e","cbCaieIoUKGKgnS8","https://ap.wps.com/l/cbCaieIoUKGKgnS8","pdf",2111405,"English","# Introduction\n## Blood components and specimen types\n## Whole blood and plasma\n## Serum\n## Course objectives and key practices","[{\"question\":\"Why is the order of draw important in phlebotomy?\",\"answer\":\"Collecting tubes in the wrong order can allow additives to carry over into subsequent tubes, causing specimen contamination and erroneous laboratory values. This can lead to misinterpretation, delayed diagnosis, or inappropriate patient management.\"},{\"question\":\"How do whole blood, plasma, and serum differ for laboratory testing?\",\"answer\":\"Whole blood contains plasma and formed elements and is collected in anticoagulant tubes to prevent clotting. Plasma is separated after clotting is prevented and is used for coagulation and many chemistry tests, while serum is collected by allowing blood to clot in an anticoagulant-free tube before centrifugation.\"},{\"question\":\"What factors can alter specimen integrity and test results?\",\"answer\":\"Improper tube selection, incorrect fill volume, and inadequate mixing can affect specimen integrity. Incorrect order of draw can further impact laboratory results, including coagulation and chemistry values.\"}]","Phlebotomy Order of Draw - Course Description | PDF",1790663574,76]