[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-657772-105":59,"doc-detail-657772-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","phase-diagram-of-ternary-cholesterolpalmitoylsphingomyelin-palmitoyloleoyl-phosphatidylcholine-mixtures-spin-label-epr-study-of-lipid-raft-formation","Phase Diagram of Ternary Cholesterol/Palmitoylsphingomyelin/ Palmitoyloleoyl-Phosphatidylcholine Mixtures - Spin-Label EPR Study of Lipid-Raft Formation","","Electron paramagnetic resonance (EPR) of spin-labeled phospholipids is used to determine the ternary phase diagram for canonical lipid-raft mixtures of cholesterol (chol), N-palmitoylsphingomyelin (PSM), and 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) at 23°C. No phase boundaries are detected for binary POPC/chol mixtures or for ternary mixtures with PSM below 24 mol%. Continuous La-to-Lo conversion is indicated. Gel–fluid coexistence appears at low cholesterol or low POPC, and La–Lb coexistence occurs for PSM/POPC. Fluid–fluid La–Lo separations are mapped and linked to raft-like Lo compositions.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/phase-diagram-of-ternary-cholesterolpalmitoylsphingomyelin-palmitoyloleoyl-phosphatidylcholine-mixtures-spin-label-epr-study-of-lipid-raft-formation/657772/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/phase-diagram-of-ternary-cholesterolpalmitoylsphingomyelin-palmitoyloleoyl-phosphatidylcholine-mixtures-spin-label-epr-study-of-lipid-raft-formation/657772.png","ImageObject",300,407,{"name":92,"@type":93},"noah","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-10-26",true,{"@type":102,"interactionType":103,"userInteractionCount":14},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What technique is used to map the ternary lipid phase diagram?","Question",{"text":112,"@type":113},"Spin-label electron paramagnetic resonance (EPR) spectroscopy is used to determine the ternary phase diagram for cholesterol, PSM, and POPC mixtures at 23°C.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Is phase separation observed in POPC/chol binary mixtures?",{"text":117,"@type":113},"No phase boundaries are found for binary POPC/chol mixtures, indicating no detectable phase separation under the reported conditions.",{"name":119,"@type":110,"acceptedAnswer":120},"What does the EPR analysis suggest about the La-to-Lo transition?",{"text":121,"@type":113},"EPR lineshapes indicate that conversion from the liquid-disordered (La) to liquid-ordered (Lo) phase occurs continuously in the studied ternary region.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},657772,1793072553,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":52,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144},1099529839121,"https://ap-avatar.wpscdn.com/davatar_994ba38a5ba835b3df7d355c54d3ed8d","Phase Diagram of Ternary Cholesterol/Palmitoylsphingomyelin/ Palmitoyloleoyl-Phosphatidylcholine Mixtures: Spin-Label EPR Study of Lipid-Raft Formation  \nIrina V. Ionova, Vsevolod A. Livshits, and Derek Marsh*  \nCentre of Photochemistry, Russian Academy of Sciences, Moscow, Russia; and Max-Planck-Institut f¨ur biophysikalische Chemie, G¨ottingen, Germany  \nABSTRACT For canonical lipid raft mixtures of cholesterol (chol), N-palmitoylsphingomyelin (PSM), and 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), electron paramagnetic resonance (EPR) of spin-labeled phospholipids—which is insensitive to domain size—is used to determine the ternary phase diagram at 23􀀁 C. No phase boundaries are found for binary POPC/chol mixtures, nor for ternary mixtures with PSM content \u003C24 mol % . EPR lineshapes indicate that conversion from the liquid-disordered (La) to liquid-ordered (Lo) phase occurs continuously in this region. Two-component EPR spectra and several tie lines attributable to coexistence of gel (Lb) and fluid phases are found for ternary mixtures with low cholesterol or low POPC content. For PSM/POPC alone, coexistence of La and Lb phases occurs over the range 50􀀃95.5 mol % PSM. A further tie line is found at 3 mol % chol with endpoints at 50 and R77 mol % PSM. For PSM/chol, Lb 􀀃Lo coexistence occurs over the range 10􀀃38 mol % chol and further tie lines are found at 4 .5 and 7 mol % POPC. Two-component EPR spectra indicative of fluid-fluid (La􀀃Lo) phase separation are found for lipid compositions: 25% \u003CPSM\u003C65%, 5%\u003Cchol\u003C30–35%, 65%>POPC>10%, and confirmed by nonlinear EPR. Tie lines are identified in the La􀀃Lo coexistence region, indicating that the fluid domains are of sufficient size to obey the phase rule. The three-phase triangle is bounded approximately by the compositions 40 and 75 mol % PSM with 10 mol % chol, and 60 mol % PSM with 25 mol % chol. These studies define the compositions of raft-like Lo phases for a minimal realistic biological lipid mixture.  \nINTRODUCTION  \nThere is now accumulating evidence that the in-plane organization of plasma membranes is heterogeneous and contains lateral nanodomains enriched in cholesterol and sphingolipids (lipid rafts) that supposedly function as platforms for self-assembly and transport of specific protein complexes involved in signal transduction, and in membrane traffic within the cell (1–3) . To gain insight into the formation and properties of such domains it is useful to study well-defined bilayer membranes that have simplified lipid compositions similar to those of cell membranes. Mixtures of a lipid with high chain-melting temperature (of which sphingolipids are representative) and cholesterol are of central interest because these are known to form the so-called liquid-ordered phase, Lo (4,5) . The latter is distinguished from the usual liquid-disordered (or La) phase by having high local orientational order, but still retaining the long-range translational disorder that is characteristic of fluid lipid membranes.  \nA recent critical survey of phospholipid/cholesterol binary phase diagrams has revealed that direct experimental evidence for the existence of Lo-phases is, however, not so extensive as is generally assumed (6) . This is especially the case for coexistence with the fluid La-phase, which is presumed to correspond with the formation of lipid raftsin biological membranes. It has long been recognized that  \nSubmitted January 13, 2012, and accepted for publication March 20, 2012.  \n*Correspondence: [dmarsh@gwdg.de](dmarsh@gwdg.de)[ ](dmarsh@gwdg.de)Editor: Paulo Almeida.  \n􀀁 2012 by the Biophysical Society 0006-3495/12/04/1856/10 $2.00  \ncoexistence of immiscible fluid phases (as opposed to gelfluid coexistence) is most likely to be of biological significance (7) . Direct evidence for coexistence of fluid La-and Lo-phase domains is somewhat more abundant in ternary systems, as found in a compendium of phase diagrams for mixtures of a high-melting lipid and a low-melting lipid with choles","cbCaijrR9rvxXmx0","https://ap.wps.com/l/cbCaijrR9rvxXmx0","pdf",593585,"English","# Abstract\n# Introduction\n## Lipid raft background and phase behavior\n## Experimental rationale for domain detection methods\n# Materials and Methods\n## Spin-label EPR approach for ternary mixtures\n# Results\n## Ternary phase diagram at 23°C\n## Binary and ternary coexistence regions\n## Gel–fluid and fluid–fluid separations\n# Discussion\n## Tie lines and phase-rule consistency\n## Raft-like Lo compositions for biological relevance","[{\"question\":\"What technique is used to map the ternary lipid phase diagram?\",\"answer\":\"Spin-label electron paramagnetic resonance (EPR) spectroscopy is used to determine the ternary phase diagram for cholesterol, PSM, and POPC mixtures at 23°C.\"},{\"question\":\"Is phase separation observed in POPC/chol binary mixtures?\",\"answer\":\"No phase boundaries are found for binary POPC/chol mixtures, indicating no detectable phase separation under the reported conditions.\"},{\"question\":\"What does the EPR analysis suggest about the La-to-Lo transition?\",\"answer\":\"EPR lineshapes indicate that conversion from the liquid-disordered (La) to liquid-ordered (Lo) phase occurs continuously in the studied ternary region.\"}]","Phase Diagram of Ternary Cholesterol/Palmitoylsphingomyelin/ Palmitoyloleoyl-Phosphatidylcholine Mixtures - Spin-Label EPR Study of Lipid-Raft Formation | PDF",1792988689,25]