[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-340591-105":59,"doc-detail-340591-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","pfkp-silencing-suppresses-tumor-growth-via-the-axl-met-axis","PFKP silencing suppresses tumor growth via the AXL-MET axis","","PFKP silencing is investigated as a non-metabolic driver of lung cancer progression through the AXL-MET signaling axis. PFKP is highly expressed in NSCLC and correlates with poor overall survival. Functional assays show that PFKP knockdown suppresses proliferation, colony formation, invasion, and migration, while nanoparticle-mediated PFKP silencing inhibits tumor growth in vivo. Mechanistically, PFKP binds AXL, increases AXL phosphorylation at Y779, promotes MET phosphorylation, and reduces glycolysis- and TCA-cycle–related proteins.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/pfkp-silencing-suppresses-tumor-growth-via-the-axl-met-axis/340591/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/pfkp-silencing-suppresses-tumor-growth-via-the-axl-met-axis/340591.png","ImageObject",300,407,{"name":92,"@type":93},"Clementine","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main finding about PFKP in NSCLC?","Question",{"text":112,"@type":113},"PFKP is highly expressed in NSCLC and is associated with poor patient survival. PFKP silencing suppresses multiple malignant behaviors of NSCLC cells and inhibits tumor growth in vivo.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does PFKP silence affect AXL and MET signaling?",{"text":117,"@type":113},"PFKP binds to AXL and promotes phosphorylation of AXL at Y779, activating AXL signaling. This activation in turn promotes phosphorylation of MET.",{"name":119,"@type":110,"acceptedAnswer":120},"What cellular processes change after PFKP silencing?",{"text":121,"@type":113},"Following PFKP silencing, several proteins involved in glycolysis, glutaminolysis, and the TCA cycle are downregulated, supporting a role for PFKP beyond glycolysis regulation.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},340591,1790440997,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},1374391974564,"https://ap-avatar.wpscdn.com/avatar/14000253aa45c000a9e?x-image-process=image/resize,m_fixed,w_180,h_180&k=1779874745381141002","Ivyspring  \nInternational Publisher  \nInternationalJournal of Biological Sciences  \n2024; 20(15): 6056-6072. doi: 10.7150/ijbs.100525  \nResearch Paper  \nPFKP silencing suppresses tumor growth via the AXL-MET axis  \nHuijie Zhao1,2*, Yuze Sun2,3*, Huijing Feng4, Jing Cai1, Yue Liu2, Yu Li2, Sijie Chen2, Zhiqing Zhou2, Yuhui Du2, Xiaofei Zeng2,5, Huan Ren6, Wenmei Su7􀀍, Qi Mei4,8􀀍, Guoan Chen2,9􀀍  \n1. Department of Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.  \n2. Department of Human Cell Biology and Genetics, Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China.  \n3. Mechanobiology Institute, National University of Singapore, Singapore.  \n4. Cancer Center, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.  \n5. National Key Laboratory for Tropical Crop Breeding, Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong 518120, China.  \n6. School of Medicine, Southern University of Science and Technology, Shenzhen, China.  \n7. Department of Oncology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.  \n8. Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, Wuhan, China.  \n9. The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen, China.  \n*These authors contributed equally to this work.  \n􀀍 Corresponding authors: Guoan Chen, Department of Human Cell Biology and Genetics, Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China, E-mail: [cheng@sustech.edu.cn](cheng@sustech.edu.cn); Qi Mei, Cancer Center, Shanxi Bethune Hospital, Shanxi Academy of Medical Science, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, China, E-mail: [borismq@163.com](borismq@163.com); Wenmei Su, Department of Oncology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China, E-mail: [suwenmei123@hotmail.com](suwenmei123@hotmail.com).  \n© The author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License ([https://creativecommons.org/licenses/by/4.0/](https://creativecommons.org/licenses/by/4.0/)). See [https://ivyspring.com/terms](https://ivyspring.com/terms) for full terms and conditions.  \nReceived: 2024.07.05; Accepted: 2024.10.25; Published: 2024.11.11  \nAbstract  \nPFKP (Phosphofructokinase, Platelet Type isoform), as an essential metabolic enzyme, contributes to the high glycolysis rates seen in cancers while its role in oncogenic pathways, especially from a non-metabolic aspect, is not fully understood. We found that PFKP was highly expressed in NSCLC and was related to poor patient survival. Knockdown of PFKP significantly inhibited cell proliferation, colony formation, invasion, and migration of NSCLC cells. Nanoparticles-mediated PFKP silencing can inhibit tumor growth in vivo. Mechanistically, we found that PFKP can bind with AXL and promote its phosphorylation at Y779, thus activating the AXL signaling pathway and promoting MET phosphorylation. In addition, several glycolysis, glutaminolysis, and TCA cycle proteins were downregulated following PFKP silencing. PFKPhas an oncogenic role in cancer progression in vitro and in vivo. Beyond its known role in glycolysis, PFKPalso has a non-metabolic function in affecting lung cancer progression by interacting with the AXL-MET axis, thus indicating a potential therapeutic target for lung cancer.  \nKeywords:","cbCaimVoLMF7fO6e","https://ap.wps.com/l/cbCaimVoLMF7fO6e","pdf",9718098,17,"English","# Abstract\n## Introduction\n## Methods\n## Results\n## Mechanistic insights\n## Discussion\n## Conclusion","[{\"question\":\"What is the main finding about PFKP in NSCLC?\",\"answer\":\"PFKP is highly expressed in NSCLC and is associated with poor patient survival. PFKP silencing suppresses multiple malignant behaviors of NSCLC cells and inhibits tumor growth in vivo.\"},{\"question\":\"How does PFKP silence affect AXL and MET signaling?\",\"answer\":\"PFKP binds to AXL and promotes phosphorylation of AXL at Y779, activating AXL signaling. This activation in turn promotes phosphorylation of MET.\"},{\"question\":\"What cellular processes change after PFKP silencing?\",\"answer\":\"Following PFKP silencing, several proteins involved in glycolysis, glutaminolysis, and the TCA cycle are downregulated, supporting a role for PFKP beyond glycolysis regulation.\"}]","PFKP silencing suppresses tumor growth via the AXL-MET axis | PDF",1790037559,43]