[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-426428-105":3,"detail-sidebar-cat-0-en-105":80,"doc-detail-426428-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":73,"head_meta":75,"extra_data":77,"updated_unix":79},105,"en","performance-fatigability-is-associated-with-differential-gene-expression-patterns-in-human-skeletal-muscle","PERFORMANCE FATIGABILITY IS ASSOCIATED WITH DIFFERENTIAL GENE EXPRESSION PATTERNS IN HUMAN SKELETAL MUSCLE","","Performance fatigability—the degree a person is limited by fatigue during physical tasks—is common among older adults and relates to physical and cognitive function. Skeletal muscle transcriptome analyses were used to uncover biological mechanisms, leveraging cross-sectional RNA sequencing data from 724 participants. Differential expression identified 1,935 genes between those with versus without performance fatigability, with pathway enrichment highlighting four muscle-related processes, suggesting links to aging-associated strength and function decline and potential targets to improve healthspan.",{"@graph":14,"@context":72},[15,34,55],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & 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was performance fatigability measured in the study?","Question",{"text":62,"@type":63},"Performance fatigability was quantified using the Pittsburgh Performance Fatigability Index (PPFI), based on the percentage of cadence decline during usual-paced activity.","Answer",{"name":65,"@type":60,"acceptedAnswer":66},"What did the RNA-seq analysis find about differential gene expression?",{"text":67,"@type":63},"A total of 1,935 differentially expressed genes were identified between participants with performance fatigability and those without, with 594 down-regulated and 1,341 up-regulated in the fatigability group.",{"name":69,"@type":60,"acceptedAnswer":70},"Which biological pathways were enriched in participants with performance fatigability?",{"text":71,"@type":63},"Gene set enrichment analysis showed up-regulation in four muscle-related pathways: collagen organization/processing, immune system activation/response, extracellular organization, and muscle cell development/function pathways.","https://schema.org",{"og:url":32,"og:type":74,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":76,"canonical":32},"index,follow",{"doc_id":78,"site_id":7},426428,1790792498,{"code":4,"msg":81,"data":82},"success",[83,87,91,95,100,105,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":84,"show_sort_weight":85,"slug":86},"Story & 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We used available cross-sectional skeletal muscle RNAseq data from the Study of Muscle, Mobility and Aging (n = 724, 55.5% Women, 86.9% White) to identify differentially expressed genes in participants with performance fatigability (PPFI>0%, n = 455, 62.8%) and those without performance fatigability (PPFI=0%, n = 269, 37.2%) . A total of  \n1,935 differentially expressed genes were identified (DESeq2; adjusted for age, sex, and batch, false discovery rate adjusted p-value \u003C 0.05) . Of these genes, 594 were down-regulated and  \n1,341 were up-regulated in participants with performance fatigability vs. those without. Gene set enrichment analysis identified up-regulation in those with performance fatigability in four key muscle-related pathways: collagen organization/processing, immune system activation/response, extracellular organization and muscle cell development/function pathways; no pathways were significantly enriched in the under-expressed genes. Upregulation of these four pathways is known to be associated with phenotypic changes in aging muscle that leads to loss of strength and function, all important for maintaining mobility in older age. Pharmaceutical or therapeutic interventions that improve muscle quality may also concomitantly reduce performance fatigability, its sequelae, and extend healthspan.  \nAbstract citation ID: igaf122.3637  \nPERFORMANCE FATIGABILITY IS ASSOCIATED WITH  \nDIFFERENTIAL GENE EXPRESSION PATTERNS IN  \nHUMAN SKELETAL MUSCLE  \nEmma Gay1, Paul Coen2, Rebecca Deek1, Brenda Diergaarde1, Samaneh Farsijani1, Michael Jurczak1, Yujia Qiao1, and Nancy Glynn1, 1. University of Pittsburgh, Pittsburgh, Pennsylvania, United States, 2. AdventHealth, Orlando, Florida, United States  \nPerformance fatigability, the degree one is limited by fatigue during a physical task, is a common trait among older adults and is associated with physical and cognitive function. Knowledge of the biological mechanisms underlying performance fatigability is limited; the skeletal muscle transcriptome may reveal important pathways to prevent or mitigate performance fatigability and promote longer healthspan. The accelerometry-based Pittsburgh Performance Fatigability Index (PPFI) quantified percentage of cadence decline (i.e., slowing down) during the usual-paced","cbCaiowBJ0aZylsw","https://ap.wps.com/l/cbCaiowBJ0aZylsw","pdf",956900,"English","# Performance fatigability and study rationale\n## Differential gene expression results\n## Enriched muscle-related pathways and implications","[{\"question\":\"How was performance fatigability measured in the study?\",\"answer\":\"Performance fatigability was quantified using the Pittsburgh Performance Fatigability Index (PPFI), based on the percentage of cadence decline during usual-paced activity.\"},{\"question\":\"What did the RNA-seq analysis find about differential gene expression?\",\"answer\":\"A total of 1,935 differentially expressed genes were identified between participants with performance fatigability and those without, with 594 down-regulated and 1,341 up-regulated in the fatigability group.\"},{\"question\":\"Which biological pathways were enriched in participants with performance fatigability?\",\"answer\":\"Gene set enrichment analysis showed up-regulation in four muscle-related pathways: collagen organization/processing, immune system activation/response, extracellular organization, and muscle cell development/function pathways.\"}]","PERFORMANCE FATIGABILITY IS ASSOCIATED WITH DIFFERENTIAL GENE EXPRESSION PATTERNS IN HUMAN SKELETAL MUSCLE | PDF",1790640555]