[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-433245-105":59,"doc-detail-433245-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","peptidoglycan-from-bifidobacterium-bifidum-g9-1-induces-iga-secretion-in-peyers-patch-derived-cells","Peptidoglycan from Bifidobacterium bifidum G9-1 induces IgA secretion in Peyer's patch-derived cells","","Peptidoglycan (PG) from the probiotic Bifidobacterium bifidum G9-1 (BBG9-1) was evaluated for its ability to induce IgA secretion in Peyer’s patch (PP)-derived cells. Heat-killed BBG9-1, but not its culture supernatant, significantly enhanced IgA secretion and promoted cell aggregation. Compared with other Gram-positive bacteria, BBG9-1 showed stronger IgA-inducing activity, and component analysis identified PG as the key driver, while lipoteichoic acid was not stimulatory. These results support PG as a candidate for modulating mucosal immune responses.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/peptidoglycan-from-bifidobacterium-bifidum-g9-1-induces-iga-secretion-in-peyers-patch-derived-cells/433245/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/peptidoglycan-from-bifidobacterium-bifidum-g9-1-induces-iga-secretion-in-peyers-patch-derived-cells/433245.png","ImageObject",300,407,{"name":92,"@type":93},"RuangKosong","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-01","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main purpose of the study?","Question",{"text":112,"@type":113},"To determine whether peptidoglycan from Bifidobacterium bifidum G9-1 can induce IgA secretion in Peyer’s patch-derived cells.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How did heat-killed BBG9-1 compare with its culture supernatant?",{"text":117,"@type":113},"Heat-killed BBG9-1 significantly enhanced IgA secretion and cell aggregation, whereas the culture supernatant did not.",{"name":119,"@type":110,"acceptedAnswer":120},"Which bacterial component was identified as responsible for IgA induction?",{"text":121,"@type":113},"Peptidoglycan (PG) isolated from BBG9-1 stimulated IgA secretion, while lipoteichoic acid (LTA) did not.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},433245,1791123844,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":24,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},962090883219,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","Bioscience of Microbiota, Food and Health Vol. 45 (1), 79–83, 2026  \nNote  \nPeptidoglycan from Bifidobacterium bifidum G9-1 induces IgA secretion in Peyer’s patch-derived cells  \nHiroaki KONISHI1, Koji YAMAMOTO2, Aki SAKATANI3, Tatsuya DOKOSHI3, Yutaka MAKIZAKI4, Yoshiki TANAKA4, Hiroshi OHNO4, Kentaro MORIICHI3 and Mikihiro FUJIYA1, 3*  \n1Department of Gastroenterology and Advanced Medical Sciences, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510, Japan  \n2Department of Physiology, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510, Japan  \n3Division of Gastroenterology, Department of Internal Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510, Japan  \n4Biofermin Pharmaceutical Co., Ltd., 7-3-4 Higashi-machi, Ibukidai, Nishi-ku, Kobe, Hyogo 651-2242, Japan  \nReceived June 11, 2025; Accepted September 11, 2025; Published online in J-STAGE December 18, 2025  \nImmunoglobulin A (IgA) plays a key role in mucosal immunity. We investigated the IgA-inducing capacity of heat-killed Bifidobacterium bifidum G9-1 (BBG9-1) using Peyer’s patch (PP)-derived cells. Heat-killed BBG9- 1, but not its culture supernatant, significantly enhanced IgA secretion and cell aggregation. Compared with other Gram-positive bacteria, BBG9-1 showed a stronger IgA-inducing effect. Component analysis revealed that peptidoglycan (PG), but not lipoteichoic acid (LTA), isolated from BBG9-1 significantly stimulated IgA secretion. These findings suggest that PG from BBG9-1 is a key factor in IgA induction, supporting its potential application in modulating mucosal immune responses.  \nKey words: immunoglobulin A, Bifidobacterium bifidum G9-1, peptidoglycan  \nImmunoglobulin A (IgA) plays a crucial role in maintaining homeostasis by regulating gut microbiota and strengthening the intestinal barrier within the mucosal immune system [1]. Secretory IgA, the predominant form in mucosal secretions, facilitates immune exclusion by preventing pathogen adhesion to epithelial surfaces and neutralizing toxins [2] . IgA modulates the microbiota composition by selectively coating specific bacteria, fostering the expansion of beneficial commensals while suppressing potential pathogens [3] . Elevated IgA levels also enhance mucosal barrier integrity by reinforcing tight junctions, thereby reducing gut permeability and limiting systemic inflammation [4] .  \nBeneficial bacteria, such as Lactobacillus and Bifidobacterium species, can stimulate IgA production via interactions with intestinal epithelial and antigen-presenting cells [5]. These bacteria activate dendritic cells and other immune cells to differentiate B cells into IgA-secreting plasma cells, thereby enhancing mucosal immunity [6]. Additionally, metabolites derived from beneficial bacteria, such as short-chain fatty acids, modulate IgA responsesand promote gut immune homeostasis [7] . However, the extent to which individual strains vary in their IgA-inducing capacity and the specific bacterial components—such as surface proteins, polysaccharides, and secreted metabolites—responsible for this activity remain unclear.  \nBifidobacterium bifidum G9-1 (BBG9-1) is a probiotic strain known to maintain the intestinal microbiota balance and exert various immunomodulatory effects. Previous investigations have demonstrated that BBG9-1 reduces total and antigen-specific IgE levels in ovalbumin-sensitized mice, suggesting potential antiallergic effects [8] . It also enhances the expression of antiinflammatory cytokines such as interleukin-10 and transforming growth factor-beta (TGF-β), as well as the regulatory T cell marker forkhead box P3 [9] . Furthermore, BBG9-1 has shown efficacy in alleviating symptoms of low-fiber diet-induced constipation and drug-induced diarrhea [10, 11] . However, its influence on intestinal IgA secretion remains to be elucidated.  \nIn this study, we evaluated the ability of heat-killed","cbCaiikBIynL38s2","https://ap.wps.com/l/cbCaiikBIynL38s2","pdf",2153805,"English","# Background\n## IgA functions in mucosal immunity\n## Prior evidence from beneficial bacteria and BBG9-1","[{\"question\":\"What is the main purpose of the study?\",\"answer\":\"To determine whether peptidoglycan from Bifidobacterium bifidum G9-1 can induce IgA secretion in Peyer’s patch-derived cells.\"},{\"question\":\"How did heat-killed BBG9-1 compare with its culture supernatant?\",\"answer\":\"Heat-killed BBG9-1 significantly enhanced IgA secretion and cell aggregation, whereas the culture supernatant did not.\"},{\"question\":\"Which bacterial component was identified as responsible for IgA induction?\",\"answer\":\"Peptidoglycan (PG) isolated from BBG9-1 stimulated IgA secretion, while lipoteichoic acid (LTA) did not.\"}]","Peptidoglycan from Bifidobacterium bifidum G9-1 induces IgA secretion in Peyer's patch-derived cells | PDF",1790663292,13]