[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-detail-150951-en":3,"doc-seo-150951-105":29,"detail-sidebar-cat-0-en-105":90},{"code":4,"msg":5,"data":6},0,"success",{"doc_id":7,"user_id":8,"nickname":9,"user_avatar":10,"doc_module":4,"category_id":11,"category_name":12,"doc_title":13,"doc_description":14,"doc_content":15,"file_id":16,"file_url":17,"file_type":18,"file_size":19,"view_count":4,"is_deleted":4,"is_public":20,"is_downloadable":20,"audit_status":20,"page_count":11,"language":21,"language_code":22,"site_id":23,"html_lang":22,"table_of_contents":24,"faqs":25,"seo_title":26,"seo_description":14,"update_tm":27,"read_time":28},150951,8796096645457,"Arica Lee","https://ap-avatar.wpscdn.com/avatar/800003749518d68ffe3?x-image-process=image/resize,m_fixed,w_180,h_180&k=1779345340919836971",7,"Healthcare","Peanut Ara h6 Detection ELISA Kit - Catalog 6042 - Research Use Only","Peanut Ara h6 Detection ELISA kit enables quantification of Ara h6, a major peanut allergen associated with IgE-mediated food allergy. The assay uses a sandwich ELISA format on a precoated 96-well plate with removable strips, with a 4-hour workflow and a standard range from 2000 to 31 pg/ml. Protocol guidance includes required sample extraction, recommended dilutions, incubation and washing steps, TMB chromogen reading at 450 nm, and storage at -20°C. Validation reports intra- and inter-assay precision plus spiking recovery.","Peanut Ara h6 Detection ELISA Kit  \nCatalog \\# 6042  \nFor Research Use Only-Not Human or Therapeutic Use  \nPRODUCT SPECIFICATIONS  \nDESCRIPTION: ELISA kit to quantify Ara h6  \nFORMAT: Precoated 96-well ELISA Plate with removeable strips  \nASSAY TYPE: Sandwich ELISA  \nASSAY TIME: 4 hours  \nSTANDARD RANGE: 2000-31 pg/ml  \nNUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate  \nSAMPLE TYPES: Liquid samples and biological fluids (pre-treatment acceptable)  \nRECOMMENDED SAMPLE DILUTIONS: 1:1 (at least)  \nCHROMOGEN: TMB (read at 450 nm)  \nSTORAGE: -20°C for 12 months  \nVALIDATION DATA: Intra-Assay (4.5-9.0%)/Inter-Assay (3.0-3.5%)/Spiking Test (101-104%)  \nNOTES: Ara h6 in samples must be extracted before starting assays  \nPeanut Ara h6 Detection ELISA Kit  \nCatalog \\# 6042  \nFor Research Use Only-Not Human or Therapeutic Use  \nINTRODUCTION  \nImmediate hypersensitivity reactions to peanuts, an IgE-mediated food allergy, have been a major public health concern for many years, particularly in westernized countries where peanut allergies can persist into adulthood. For allergic patients, avoidance currently remains the only viable option (1) .  \nEleven potentially important peanut allergens have been identified. Ara h1, Ara h2, Ara h3, and Ara h6 have been designated the major peanut allergens. Ara h2 and Ara h6, two highly related 2S albumins belonging to seed storage proteins, especially contribute to the development of allergic reactions (2) . Ara h2 contains ten independent IgE-binding linear epitopes. Two isoforms, Ara h 2.01 and Ara h 2 .02, were identified respectively as a 17 and 19-kDa doublet on SDS-PAGE. The larger isoform containing twelve extra amino acids including a duplication of a strong IgE-binding sequence, DPYSPS, can bind more IgE antibodies (3, 4) . Ara h6 has a molecular weight of 14.5 kDa and is 59 % homologous to Ara h2 . It also possesses heat and digestion stability, a protease-stable core, and allergenic potency similar to that of Ara h2 (5-6) .  \nThe methods used to analyze allergens in foods are often divided into either DNA-based methods or protein-based methods. Immunoassays are one example of protein-based methods, and they are used in many industries for detecting allergens in foods. Enzyme-linked immunosorbent assays (ELISA) are commonly used for validating contamination of allergens in food products because of their high-precision, easy handling, and good potential for standardization (7) .  \nAs a peanut allergen, Ara h6 can be a useful analytical target for validating peanut-free foods. This application is not limited to only food testing either, as interestingly, serum Ara h6 levels correlate with peanut allergy tolerance in patients and can be a useful marker of tolerance levels in therapeutic studies (8) .  \nChondrex, Inc provides an Ara h6 detection ELISA kit for analyzing food quality as well as for studying the distribution and dynamics of Ara h6 . Please visit [www.chondrex.com](www.chondrex.com) or contact [support@chondrex.com](support@chondrex.com) for more information.  \nKIT COMPONENTS  \n\n| Item | Quantity | Amount | Storage |\n| --- | --- | --- | --- |\n| Standard Ara h6 (60421) | 1 vial | 2000 pg, lyophilized | -20°C |\n| Anti-Ara h6 Detection Antibody (60423) | 1 vial | 100 µl | -20°C |\n| Solution B-Sample/Standard/Detection Antibody Dilution Buffer (67015) | 1 bottle | 50 ml | -20°C |\n| Solution D-Streptavidin Peroxidase Dilution Buffer (9055) | 2 bottles | 20 ml | -20°C |\n| Streptavidin Peroxidase (9029) | 2 vials | 50 µl | -20°C |\n| TMB Solution (90023) | 2 vials | 0.2 ml | -20°C |\n| Chromogen Dilution Buffer (90022) | 1 bottle | 20 ml | -20°C |\n| Stop Solution-2N Sulfuric Acid (9016) | 1 bottle | 10 ml | -20°C |\n| Wash Buffer, 20X (9005) | 1 bottle | 50 ml | -20°C |\n| Anti-Ara h6 Antibody Coated ELISA Plate (Red) | 1 each | 96-well (8-well strips x 12) | -20°C |\n\nASSAY OUTLINE  \nAdd 100 ml of diluted standards or samples in wells  \nIncubate at room temperature for 2 hours. Wash plates","cbCaierGfpS8DwpA","https://ap.wps.com/l/cbCaierGfpS8DwpA","pdf",344229,1,"English","en",105,"# Product Specifications\n## Description and Assay Format\n## Performance and Storage\n# Kit Components\n# Assay Outline and Procedure\n## Assay Outline\n## Notes Before Using Assay\n# Plate Mapping\n## Standards and Samples","[{\"question\":\"What is the purpose of the Peanut Ara h6 Detection ELISA kit?\",\"answer\":\"It quantifies Ara h6 for research applications such as validating peanut-free food status and studying Ara h6 distribution and dynamics.\"},{\"question\":\"What assay type and typical assay time does the kit use?\",\"answer\":\"The kit uses a sandwich ELISA format and requires about 4 hours to complete.\"},{\"question\":\"How should samples be handled before starting the assay?\",\"answer\":\"Ara h6 in samples must be extracted before beginning assays, and standards/samples are recommended to be run in duplicate.\"}]","Peanut Ara h6 Detection ELISA Kit - Catalog 6042 - Research Use Only | PDF",1787830072,18,{"code":4,"msg":30,"data":31},"ok",{"site_id":23,"language":22,"slug":32,"title":13,"keywords":33,"description":14,"schema_data":34,"social_meta":85,"head_meta":87,"extra_data":89,"updated_unix":27},"peanut-ara-h6-detection-elisa-kit-catalog-6042-research-use-only","",{"@graph":35,"@context":84},[36,53,67],{"@type":37,"itemListElement":38},"BreadcrumbList",[39,43,47,50],{"item":40,"name":41,"@type":42,"position":20},"https://docshare.wps.com","Home","ListItem",{"item":44,"name":45,"@type":42,"position":46},"https://docshare.wps.com/document/","Document",2,{"item":48,"name":12,"@type":42,"position":49},"https://docshare.wps.com/document/healthcare/",3,{"item":51,"name":13,"@type":42,"position":52},"https://docshare.wps.com/document/peanut-ara-h6-detection-elisa-kit-catalog-6042-research-use-only/150951/",4,{"url":51,"name":13,"@type":54,"author":55,"headline":13,"publisher":57,"fileFormat":60,"inLanguage":22,"description":14,"dateModified":61,"datePublished":61,"encodingFormat":60,"isAccessibleForFree":62,"interactionStatistic":63},"DigitalDocument",{"name":9,"@type":56},"Person",{"url":40,"name":58,"@type":59},"DocShare","Organization","application/pdf","2026-08-27",true,{"@type":64,"interactionType":65,"userInteractionCount":4},"InteractionCounter",{"@type":66},"ViewAction",{"@type":68,"mainEntity":69},"FAQPage",[70,76,80],{"name":71,"@type":72,"acceptedAnswer":73},"What is the purpose of the Peanut Ara h6 Detection ELISA kit?","Question",{"text":74,"@type":75},"It quantifies Ara h6 for research applications such as validating peanut-free food status and studying Ara h6 distribution and dynamics.","Answer",{"name":77,"@type":72,"acceptedAnswer":78},"What assay type and typical assay time does the kit use?",{"text":79,"@type":75},"The kit uses a sandwich ELISA format and requires about 4 hours to complete.",{"name":81,"@type":72,"acceptedAnswer":82},"How should samples be handled before starting the assay?",{"text":83,"@type":75},"Ara h6 in samples must be extracted before beginning assays, and standards/samples are recommended to be run in duplicate.","https://schema.org",{"og:url":51,"og:type":86,"og:title":13,"og:site_name":58,"og:description":14},"article",{"robots":88,"canonical":51},"index,follow",{"doc_id":7,"site_id":23},{"code":4,"msg":5,"data":91},[92,96,100,104,109,114,117,122,127,130,134],{"id":20,"doc_module":4,"doc_module_name":45,"category_name":93,"show_sort_weight":94,"slug":95},"Story & Novel",90,"story-novel",{"id":46,"doc_module":4,"doc_module_name":45,"category_name":97,"show_sort_weight":98,"slug":99},"Literature",80,"literature",{"id":52,"doc_module":4,"doc_module_name":45,"category_name":101,"show_sort_weight":102,"slug":103},"Exam",70,"exam",{"id":105,"doc_module":4,"doc_module_name":45,"category_name":106,"show_sort_weight":107,"slug":108},5,"Comic",60,"comic",{"id":110,"doc_module":4,"doc_module_name":45,"category_name":111,"show_sort_weight":112,"slug":113},6,"Technology",50,"technology",{"id":11,"doc_module":4,"doc_module_name":45,"category_name":12,"show_sort_weight":115,"slug":116},40,"healthcare",{"id":118,"doc_module":4,"doc_module_name":45,"category_name":119,"show_sort_weight":120,"slug":121},8,"Research & Report",30,"research-report",{"id":123,"doc_module":4,"doc_module_name":45,"category_name":124,"show_sort_weight":125,"slug":126},9,"Religion & Spirituality",20,"religion-spirituality",{"id":125,"doc_module":4,"doc_module_name":45,"category_name":128,"show_sort_weight":125,"slug":129},"World Cup","world-cup",{"id":131,"doc_module":4,"doc_module_name":45,"category_name":132,"show_sort_weight":131,"slug":133},10,"Lifestyle","lifestyle",{"id":135,"doc_module":4,"doc_module_name":45,"category_name":136,"show_sort_weight":105,"slug":137},19,"General","general"]