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C6® perfusion cultivation - Thesis submitted for PhD at Wageningen University - 2014","","Doctoral thesis titled \"PAT for PER. C6® perfusion cultivation\" from Wageningen University, submitted for the degree of doctor in 2014. The work develops multivariate PAT solutions and multivariate data analysis methods to support early bioprocess development for biopharmaceutical cultivation. It characterizes apoptosis in PER. C6® batch and perfusion cultures, evaluates robustness of the PER. C6® cell line for adenovirus propagation, and establishes PAT tools for perfusion cultivation using dielectric and near-infrared spectroscopy. The thesis includes summaries in Dutch, English, and French and contains references.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/pat-for-per-c6-perfusion-cultivation-thesis-submitted-for-phd-at-wageningen-university-2014/289849/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/pat-for-per-c6-perfusion-cultivation-thesis-submitted-for-phd-at-wageningen-university-2014/289849.png","ImageObject",300,407,{"name":92,"@type":93},"Logic","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-18","2026-09-17",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main focus of this thesis?","Question",{"text":112,"@type":113},"The thesis focuses on PAT for PER. C6® perfusion cultivation, including methods for monitoring and analyzing bioprocesses during biopharmaceutical cultivation.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which data analysis approaches are covered?",{"text":117,"@type":113},"It presents multivariate PAT solutions and multivariate data analysis as tools for early bioprocess development data.",{"name":119,"@type":110,"acceptedAnswer":120},"How does the thesis support perfusion cultivation monitoring?",{"text":121,"@type":113},"It describes PAT tools based on dielectric and near-infrared spectroscopy for a perfusion PER. C6® cultivation process.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},289849,1789637875,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":129,"read_time":144,"preview_page_count":4},1099513958762,"https://ap-avatar.wpscdn.com/avatar/1000023916a998db790?x-image-process=image/resize,m_fixed,w_180,h_180&k=1784791008015729253","PAT for PER.C6® perfusion cultivation  \nSarah M. Mercier  \nPAT for PER. C6 ® perfusion cultivation  \nSarah M. Mercier  \nThesis committee  \nPromotor  \nProf. Dr R. H. Wijffels  \nProfessor of Bioprocess Engineering Wageningen University  \nCo-promotor Dr M. Streefland  \nAssistant professor, Bioprocess Engineering Wageningen University  \nOther members  \nProf. Dr M.M. van Oers, Wageningen University  \nProf. Dr A.S. Rathore, Indian Institute of Technology, New Delhi, India Prof. Dr H. F.J. Savelkoul, Wageningen University  \nDr N. Vriezen, Synthon, Nijmegen, The Netherlands  \nThis research was conducted under the auspices of the Graduate School VLAG  \n(Advanced studies in Food Technology, Agrobiotechnology, Nutrition and Health Sciences) .  \nPAT for PER. C6 ® perfusion cultivation  \nSarah M. Mercier  \nThesis  \nsubmitted in fulfillment of the requirements for the degree of doctor at Wageningen University by the authority of the Rector Magnificus Prof. Dr M.J. Kropff,  \nin the presence of the  \nThesis Committee appointed by the Academic Board to be defended in public  \non Friday 14 November 2014  \nat 1:30 [p. m. in](p. m. in) the Aula.  \nSarah M. Mercier  \nPAT for PER. C6® perfusion cultivation  \n156 pages.  \nPhD thesis, Wageningen University, Wageningen, NL (2014) With references, with summaries in Dutch, English and French  \nISBN 978-94-6257-139-6  \nIl faut bien que je supporte deux ou trois chenilles si je veux connaître les papillons.  \nFrom Le Petit Prince, Antoine de Saint-Exupery  \nTable of contents  \nChapter 1 Introduction and thesis outline 9  \nChapter 2 Multivariate PAT solutions for biopharmaceutical cultivation: 19  \ncurrent progress and limitations  \nChapter 3 Multivariate data analysis as a PAT tool for early bioprocess 39  \ndevelopment data  \nChapter 4 Characterization of apoptosis in PER. C6® batch and 61  \nperfusion cultures  \nChapter 5 Robustness of PER. C6® cell line for Adenovirus propagation 83  \nChapter 6 PAT tools for a perfusion PER. C6® cultivation process: 103  \ndielectric and near-infrared spectroscopy  \nChapter 7 General discussion 123  \nSummary 137  \nSamenvatting 141  \nRésumé 145  \nAcknowledgements 149  \nCurriculum vitae 153  \nList of publications 154  \nTraining activities 155  \nChapter 1  \nIntroduction and thesis outline","cbCaikhWFjBbmq94","https://ap.wps.com/l/cbCaikhWFjBbmq94","pdf",1737251,161,"English","# Chapter 1 Introduction and thesis outline\n# Chapter 2 Multivariate PAT solutions for biopharmaceutical cultivation: current progress and limitations\n# Chapter 3 Multivariate data analysis as a PAT tool for early bioprocess development data\n# Chapter 4 Characterization of apoptosis in PER. C6® batch and perfusion cultures\n# Chapter 5 Robustness of PER. C6® cell line for Adenovirus propagation\n# Chapter 6 PAT tools for a perfusion PER. C6® cultivation process: dielectric and near-infrared spectroscopy\n# Chapter 7 General discussion\n# Summary\n# Samenvatting\n# Résumé\n# Acknowledgements\n# Curriculum vitae\n# List of publications\n# Training activities","[{\"question\":\"What is the main focus of this thesis?\",\"answer\":\"The thesis focuses on PAT for PER. C6® perfusion cultivation, including methods for monitoring and analyzing bioprocesses during biopharmaceutical cultivation.\"},{\"question\":\"Which data analysis approaches are covered?\",\"answer\":\"It presents multivariate PAT solutions and multivariate data analysis as tools for early bioprocess development data.\"},{\"question\":\"How does the thesis support perfusion cultivation monitoring?\",\"answer\":\"It describes PAT tools based on dielectric and near-infrared spectroscopy for a perfusion PER. C6® cultivation process.\"}]","PAT for PER. C6® perfusion cultivation - Thesis submitted for PhD at Wageningen University - 2014 | PDF",406]