[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-662946-105":59,"doc-detail-662946-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","panel-design-golden-rule","Panel Design - Golden Rule","","Panel design for multicolor flow cytometry explains how to build a reagent antibody panel to identify specific cell populations in a sample. It covers defining experimental targets, selecting literature or prior knowledge for cell identification, and establishing a consecutive gating strategy. The material describes gating as subset definition on scatter plots or histograms, outlines principles such as minimizing spillover, choosing antigen-fluorochrome combinations, spreading antigens across lasers, and using appropriate controls, with guidance for instrument-dependent detector/laser capabilities.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/panel-design-golden-rule/662946/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/panel-design-golden-rule/662946.png","ImageObject",300,407,{"name":92,"@type":93},"Syukur Setiap Hari","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-10-28",true,{"@type":102,"interactionType":103,"userInteractionCount":8},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What must be defined before starting a panel experiment?","Question",{"text":112,"@type":113},"Define the target cells, how those cells are identified (from literature or prior experience), and a consecutive gating strategy to produce clear, interpretable data.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What does the gating process do in multicolor flow cytometry?",{"text":117,"@type":113},"Gating selects regions on scatter plots or histograms to identify and define subsets of cell populations for further analysis, representing fractions of cells with specified properties.",{"name":119,"@type":110,"acceptedAnswer":120},"What are key principles for designing a panel?",{"text":121,"@type":113},"Know the instrument, determine antigen expression and density, choose antigen-fluorochrome combinations, minimize spillover, spread antigens across lasers, consider tandem dye limitations, and use appropriate controls.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},662946,1793223643,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":8,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":31,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":129,"read_time":143},962093541965,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","| \u003Cbr>\u003Cbr>\u003Cbr>\u003Cbr>\u003Cbr>\u003Cbr>\u003Cbr>Introduc)on to Panel Design and Sample\u003Cbr>\u003Cbr>|\n| --- |\n| \u003Cbr>Pre para)on Diana Ordonez April 18, 2016\u003Cbr>\u003Cbr>\u003Cbr>\u003Cbr>\u003Cbr>\u003Cbr>\u003Cbr>\u003Cbr>\u003Cbr>|\n\nPanel is a combina)on of reagents, usually an)bodies, that recognize surface and intracellular molecules allowing the iden)fica)on of cell popula)ons in a given sample/)ssue .  \nSp. leen  \nBefore star)ng your panel, define:  \n-­‐Which cells are the target of the experiment  \n-­‐Find out how these cells are iden)fied in the literature, or by past experience  \n-­‐Define a consecu)ve ga)ng strategy in order to obtain clear and interpretable data.  \n2 April 23, 2016  \nThe process of ga)ng is simply selec)ng an area on the scaOer plots or histograms toiden)fy and define subsets of popula)ons for further analysis .  \nSubsets defined by a gate are exactly like subsets in biology: they represent a frac)on of cells with specified proper)es .  \nB220  \nCD5  \nT cells  \nCD4  \nCD62L CD62L  \nCD4+ cells  \nFoxp3  \nCD44 CD4  \nCD8+ cells  \nCD44  \nIFNy  \nCD8  \n8 an)bodies  \n13 popula)ons  \n3  \n23/04/16  \nHow to prepare an An\\#body Panel?  \n4  \n23/04/16  \nGolden Rule  \n“Be pa\\#ent in developing/op\\#mizing  \nyour panel”  \n5  \n23/04/16  \nPrinciples of Panel Design  \n• Know your instrument  \n• Determine an)gen expression and density  \n• Determine An)gen-­‐Fluorochrome Combos  \n• Minimize spillover  \n• Spread an)gens across lasers  \n• Consider technical limita)ons of tandem dyes  \n• Use appropriate controls  \n• Tips to improve your panel  \n• Mul\\#-­‐Parameter Flow Cytometry Experiment  \n6  \n23/04/16  \n• Laser and detectors: the type and number of laser and detectors dictate whether the op)cal system can excite and detect a given fluorochrome .  \nBD FACScanto II: 405-­‐488-­‐633 Fluorescence detectors: 8  \nBD Accuri C6: 488-­‐633  \nFluorescence detectors: 4 BD LSRFortessa:  \n355-­‐405-­‐488-­‐561-­‐633  \nFluorescence detectors: 16  \n7  \n23/04/16  \n8  \n23/04/16  \nRankings were determined by comparing the staining index of cells stained with several an)body clones run on a variety of flow cytometers .  \n[https://www.bdbiosciences.com/documents/Fluorochrome-Chart-Relative-Brightness.pdf](https://www.bdbiosciences.com/documents/Fluorochrome-Chart-Relative-Brightness.pdf)  \n9  \n23/04/16  \n• Fluorochromes Brightness: Available dyes could be rank according to their brightness on a given instrument.  \nStain Index: “How resolvable the signal is over the unstained cell popula)on”  \nSI=  \nμ Posi)ve-­‐ μ Background  \n2 x SD Background  \nWhen the same an)body is conjugated to various dyes, their stain indexes can be compared to determine the rela)ve brightness of the dyes on a par)cular instrument.  \nMaecker and trotter, Selecting reagents for multicolor flow cytometry. BD Application Note  \n10 April 23, 2016","cbCaisSk9KHmGbFG","https://ap.wps.com/l/cbCaisSk9KHmGbFG","pdf",8013346,"English","# Introduc)on to Panel Design and Sample\n## Prepara)on and target definition\n## Gating process and subset interpretation\n## How to prepare an An)body Panel\n## Golden Rule\n## Principles of Panel Design\n## Laser and detectors considerations\n## Stain Index and fluorochrome ranking","[{\"question\":\"What must be defined before starting a panel experiment?\",\"answer\":\"Define the target cells, how those cells are identified (from literature or prior experience), and a consecutive gating strategy to produce clear, interpretable data.\"},{\"question\":\"What does the gating process do in multicolor flow cytometry?\",\"answer\":\"Gating selects regions on scatter plots or histograms to identify and define subsets of cell populations for further analysis, representing fractions of cells with specified properties.\"},{\"question\":\"What are key principles for designing a panel?\",\"answer\":\"Know the instrument, determine antigen expression and density, choose antigen-fluorochrome combinations, minimize spillover, spread antigens across lasers, consider tandem dye limitations, and use appropriate controls.\"}]","Panel Design - Golden Rule | PDF",126]