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Functional assays with lentiviral knockdown in FaDu and CNE-2Z cells assessed proliferation, cell-cycle arrest at G2/M, and apoptosis, supported by immunohistochemistry, qRT-PCR, and intracellular signaling protein profiling.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & 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\nPan-cancer analysis of NUDT21 and its effect on the proliferation of human head and neck squamous cell carcinoma  \nWenjing Liu1, Yingna Pang2, Xiaolu Yu2, Doudou Lu4, Yating Yang5, Fandi Meng5, Chengbi Xu2,*, Ling Yuan3,*, Yi Nan1,*  \n1 Key Laboratory of Ningxia Minority Medicine Modernization Ministry of Education, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China  \n2 Department of Otolaryngology Head and Neck Surgery, The Second Hospital of Jilin University, Changchun 130000, China  \n3College of Pharmacy, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China 4Clinical Medical College, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China 5Traditional Chinese Medicine College, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China  \n*Equal contribution  \nCorrespondence to: Chengbi Xu, Ling Yuan, Yi Nan; [email:](email: xucb@jlu.edu.cn)[ ](email: xucb@jlu.edu.cn)[xucb@jlu.edu.cn](email: xucb@jlu.edu.cn), [20080017@nxmu.edu.cn](20080017@nxmu.edu.cn), [20080011@nxmu.edu.cn](20080011@nxmu.edu.cn)[ ](20080011@nxmu.edu.cn)[Keywords:](Keywords: NUDT21)[ NUDT21](Keywords: NUDT21), pan-cancer research, HHNSCC, immune microenvironment, apoptosis  \nReceived: November 1, 2023 Accepted: January 8, 2024 Published: February 12, 2024  \nCopyright: © 2024 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.  \nABSTRACT  \nBackground: Based on bioinformatics research of NUDT21 in pan-cancer, we aimed to clarify the mechanism of NUDT21 in HHNC by experiment.  \nMethods: The correlation between differential expression of NUDT21 in pan-cancer and survival prognosis, genomic instability, tumor stemness, DNA repair, RNA methylation and with immune microenvironment were analyzed by the application of different pan-cancer analysis web databases. In addition, immunohistochemistry staining and genetic detection of NUDT21 in HHNCC tumor tissues by immunohistochemistry and qRT-PCR. Then, through in vitro cell experiments, NUDT21 was knocked down by lentivirus to detect the proliferation, cycle, apoptosis of FaDu and CNE-2Z cells, and finally by PathScan intracellular signaling array reagent to detect the apoptotic protein content.  \nResults: Based on the pan-cancer analysis, we found that elevated expression of NUDT21 in most cancers was significantly correlated with TMB, MSI, neoantigens and chromosomal ploidy, and in epigenetics, elevated NUDT21 expression was strongly associated with genomic stability, mismatch repair genes, tumor stemness, and RNA methylation. Based on immunosuppressive score, we found that NUDT21 plays an essential role in the immunosuppressive environment by suppressing immune checkpointing effect in most cancers. In addition, using HHNSCC as a study target, PCR and pathological detection of NUDT21 in tumor tissues was significantly increased than that in paracancerous normal tissues. In vitro cellular assays, silencing NUDT21 inhibited proliferation and promoted apoptosis in FaDu and CNE-2Z cells, and blocked the cell cycle in the G2/M phase. Therefore, the experiments confirmed that NUDT21 promotes the proliferation of FaDu by suppressing the expression of apoptotic.  \nINTRODUCTION  \nCancer, as the main cause of global morbidity and mortality, has brought huge health and economic burdens to society. Currently, cancer treatment strategies encompass surgical resection, radiotherapy, adjuvant chemotherapy, targeted therapy, and immunotherapy. While these approaches have yielded some results, patients still face unsatisfactory prognoses and quality of life due to toxic side effects and drug resistance [1] . Hence, it is crucial to urgently i","cbCaiuTyxQvIL9ri","https://ap.wps.com/l/cbCaiuTyxQvIL9ri","pdf",5985264,23,"English","# Abstract\n## Background\n## Methods\n## Results\n# Introduction","[{\"question\":\"What was the study aim regarding NUDT21 in HHNSCC?\",\"answer\":\"To clarify the mechanism of NUDT21 in HHNSCC by combining pan-cancer bioinformatics with experimental validation.\"},{\"question\":\"How was NUDT21 function assessed in HHNSCC models?\",\"answer\":\"NUDT21 was knocked down using lentivirus in FaDu and CNE-2Z cells, followed by assays for proliferation, cell-cycle progression, and apoptosis, supported by immunohistochemistry and qRT-PCR.\"},{\"question\":\"What did the pan-cancer analysis show about NUDT21 expression?\",\"answer\":\"Elevated NUDT21 expression correlated with TMB, MSI, neoantigens, chromosomal ploidy, and—at the epigenetic level—with genomic stability, mismatch repair genes, tumor stemness, and RNA methylation.\"}]","Pan-cancer analysis of NUDT21 and its effect on the proliferation of human head and neck squamous cell carcinoma - Research Paper | PDF",1789987698,58]