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Exosomes from fetal chorion trophoblast cells restore P-glycoprotein efflux function in inflammation-impaired maternal decidual cells at the feto-maternal interface. CTCs preserve P-gp expression under inflammatory stress, while DECs downregulate transporter genes and proteins.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/p-glycoprotein-exofection-between-fetal-and-maternal-cells-as-a-mechanism-of-intercellular-material-transfer-at-the-feto-maternal-interface/451471/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/p-glycoprotein-exofection-between-fetal-and-maternal-cells-as-a-mechanism-of-intercellular-material-transfer-at-the-feto-maternal-interface/451471.png","ImageObject",300,407,{"name":92,"@type":93},"McQueen","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-07","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is exofection, according to the document?","Question",{"text":112,"@type":113},"Exofection is defined as the delivery of functional protein cargo by extracellular vesicles to recipient cells that have lost the ability to express or utilize that protein. It enables a transient, non-genetic restoration of protein function in recipient cells.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How do fetal chorion trophoblast cell exosomes affect maternal decidual cells?",{"text":117,"@type":113},"Fetal chorion trophoblast cell–derived exosomes restore P-glycoprotein efflux transporter function in inflammation-impaired maternal decidual cells. Proteomic analysis indicates the cargo is packaged as stable P-gp and taken up via clathrin- and heparan sulfate–dependent pathways.",{"name":119,"@type":110,"acceptedAnswer":120},"What evidence supports functional delivery of P-glycoprotein?",{"text":121,"@type":113},"Delivery of CTC exosomes reinstates P-gp abundance and efflux capacity in LPS-stimulated or P-gp-deficient decidual cells. Effects are shown using calcein efflux assays and immunofluorescence, and in pregnant P-gp knockout mice exosome treatment restores systemic clearance of tacrolimus.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},451471,1791070370,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},5909890329169,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","bioRxiv preprint doi: [https://doi.org/10.64898/2026.01.04.697556](https://doi.org/10.64898/2026.01.04.697556); this version posted January 4, 2026. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made  \navailable under aCC-BY-NC-ND 4.0 International license.  \n1 P-glycoprotein exofection between fetal and maternal cells as a mechanism of intercellular  \n2 material transfer at the feto maternal interface  \n3 Madhuri Tatiparthy, Amanda Wang, Vineeth Mahajan, Pilar Flores-Espinosa, Emmanuel  \n4 Amabebe, Tilu Jain Thomas, Xiao-Ming Wang, Lauren S Richardson, Ramkumar Menon†, 5 Ananth K Kammala†,  \n6 Affiliations:  \n7 Division of Basic Science and Translational Research, Department of Obstetrics & Gynecology, 8 The University of Texas Medical Branch at Galveston, Galveston, TX, USA.  \n9  \n10 †Corresponding authors 11  \n12 Ananth kumar Kammala, M Pharm, PhD  \n13 Professor, Department of Obstetrics and Gynecology,  \n14 Director, Division of Basic Science and Translational Research  \n15 The University of Texas Medical Branch at Galveston  \n16 MRB 11-138, 301 University Blvd, Galveston, TX 77555  \n17 Ph (Office) -409-772-7596; Ph (Cell)– 346-366-3638  \n18 [E-mail:](E-mail: ankammal@utmb.edu)[ ](E-mail: ankammal@utmb.edu)[ankammal@utmb.edu](E-mail: ankammal@utmb.edu)[ ](E-mail: ankammal@utmb.edu)19  \n20  \n21 Ramkumar Menon, MS, PhD  \n22 Professor, Department of Obstetrics and Gynecology,  \n23 Director, Division of Basic Science and Translational Research  \n24 The University of Texas Medical Branch at Galveston  \n25 MRB 11-138, 301 University Blvd, Galveston, TX 77555  \n26 Ph (Office) -409-772-7596; Ph (Cell) -615-335-5564  \n27 E-mail: [ram.menon@utmb.edu](ram.menon@utmb.edu); [ra2menon@utmb.edu](ra2menon@utmb.edu)[ ](ra2menon@utmb.edu)28  \n29  \n30  \n31  \n32 One Sentence Summary: Exofection is identified as a novel mechanism of transient cellular  \n33 engineering via exosome-mediated functional protein delivery between cells.  \nbioRxiv preprint doi: [https://doi.org/10.64898/2026.01.04.697556](https://doi.org/10.64898/2026.01.04.697556); this version posted January 4, 2026. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made  \navailable under aCC-BY-NC-ND 4.0 International license.  \n34  \n35 Abstract: Cells can recover lost protein functions through a process we term exofection, in  \n36 which extracellular vesicles deliver functional molecular cargo to recipient cells and transiently  \n37 reprogram their activity. Here we show that exosomes derived from fetal chorion trophoblast  \n38 cells (CTCs) restore P-glycoprotein (P-gp) efflux transporter function in inflammation-impaired  \n39 maternal decidual cells (DECs) at the feto-maternal interface. CTCs maintain high P-gp  \n40 expression under inflammatory stress, whereas DECs exhibit marked downregulation of  \n41 transporter genes and proteins. Proteomic analysis revealed that CTC-derived exosomes package 42 P-gp as a stable cargo that enters DECs through clathrin-and heparan sulfate–dependent uptake  \n43 pathways. Delivery of CTC exosomes reinstated P-gp abundance and efflux capacity in LPS- 44 stimulated or P-gp-deficient DECs, as shown by calcein efflux and immunofluorescence assays.  \n45 In pregnant P-gp knockout mice, exosome treatment restored systemic clearance of the P-gp  \n46 substrate tacrolimus and improved pharmacokinetic parameters. These findings establish  \n47 exofection as a naturally occurring mechanism of transporter rescue at the feto-maternal  \n48 interface, where fetal exosomes compensate for inflammation-induced maternal loss of efflux  \n49 capacity. By restoring P–gp–mediated barrier function, exofection provides a protective strategy  \n50 that limits the accumulation of xenobiotics and cytokines in mater","cbCaioKczhLUSEYy","https://ap.wps.com/l/cbCaioKczhLUSEYy","pdf",5548124,44,"English","# Abstract\n## One Sentence Summary\n## Introduction","[{\"question\":\"What is exofection, according to the document?\",\"answer\":\"Exofection is defined as the delivery of functional protein cargo by extracellular vesicles to recipient cells that have lost the ability to express or utilize that protein. It enables a transient, non-genetic restoration of protein function in recipient cells.\"},{\"question\":\"How do fetal chorion trophoblast cell exosomes affect maternal decidual cells?\",\"answer\":\"Fetal chorion trophoblast cell–derived exosomes restore P-glycoprotein efflux transporter function in inflammation-impaired maternal decidual cells. Proteomic analysis indicates the cargo is packaged as stable P-gp and taken up via clathrin- and heparan sulfate–dependent pathways.\"},{\"question\":\"What evidence supports functional delivery of P-glycoprotein?\",\"answer\":\"Delivery of CTC exosomes reinstates P-gp abundance and efflux capacity in LPS-stimulated or P-gp-deficient decidual cells. Effects are shown using calcein efflux assays and immunofluorescence, and in pregnant P-gp knockout mice exosome treatment restores systemic clearance of tacrolimus.\"}]","P-glycoprotein exofection between fetal and maternal cells as a mechanism of intercellular material transfer at the feto-maternal interface | PDF",1790735181,111]